O27126 (RPOA2_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit A''
EC=2.7.7.6

Gene names

Name:	rpoA2
Ordered Locus Names:	MTH_1052

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00411
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00411
Subunit structure	This RNA polymerase is composed of four subunits: A',A'',B' and B'' By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	DNA-directed RNA polymerase subunit A'' HAMAP MF_00411		PRO_0000074017
Regions
	Region	1 – 68	68	Unknown HAMAP MF_00411
	Region	69 – 451	383	DNA-directed RNA polymerase subunit A" HAMAP MF_00411

Sequences

	Sequence		Length	Mass (Da)
	O27126 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 742EC583541A9060	FASTA	451	51,004
	10 20 30 40 50 60 MQDIIGKIED YSSKNGILLP DPVVEYVARI ADEEKLKEPE LQEMVRLFSR IAERNQGLDD 70 80 90 100 110 120 DELLDAVEDD YQRILKVQEL VKRKRARFPP KLIEDIAEVM KKHELSDDEL DELIRRVRRA 130 140 150 160 170 180 YDRARVEAGE AVGTVAAQSV GEPGTQMTMR TFHYAGVAEL NVTLGLPRLI EIVDARKKIS 190 200 210 220 230 240 TPTMSIYFEG DLRYDEEFVR RKANKIGKST LNDVLKNFSI QYADMSVVAE LDEEKIQEKH 250 260 270 280 290 300 LEYDEILAKV EKTFKKVEID NNILRFEPPK PTIRELRLLA DKVRKLQISG VKNIGKVVIR 310 320 330 340 350 360 KEDDEWVIHT EGSNLGAVLK EEGVDKVRTT TNDIHEIETV LGIEAARNAI IHEAKRTMEE 370 380 390 400 410 420 QGLTVDIRHI MLVADMMTAD GSVKSIGRHG ISGEKASVLA RASFEETGKH LLRASIRGEV 430 440 450 DHLTGIIENI IIGQPIPLGT GSVSVVMKER K

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85543.1.
PIR	H69006.
RefSeq	NP_276182.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27126.
SMR	O27126. Positions 126-177.
ModBase	Search...
Protein-protein interaction databases
STRING	O27126.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471460.
GenomeReviews	Gene locus MTH_1052 in contig AE000666_GR.
KEGG	mth:MTH1052.
NMPDR	fig|187420.1.peg.1037.
Phylogenomic databases
eggNOG	arNOG04558.
HOGENOM	HBG297327.
OMA	RPIGRHG.
ProtClustDB	PRK14897.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1052-MONOMER.
Family and domain databases
HAMAP	MF_00411. RNApol_arch_A. Fused. [Tree]
InterPro	IPR012757. DNA-dir_RpoA2. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03042.
Pfam	PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR02389. RNA_pol_rpoA2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA2_METTH

Accession

Primary (citable) accession number: O27126

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program