O27125 (RPOA1_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit A'
EC=2.7.7.6

Gene names

Name:	rpoA1
Ordered Locus Names:	MTH_1051

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	870 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Subunit structure	M.thermoautotrophicum RNA polymerase is composed of four subunits: A',A'',B' and B''.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 870	870	DNA-directed RNA polymerase subunit A'		PRO_0000074004
Regions
	Zinc finger	60 – 103	44	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	O27125 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 05BB2C2C80CFD211	FASTA	870	98,078
	10 20 30 40 50 60 MRGILKKISQ INFGLMSPED IRKMSVTQIV TPDTYDEDGY PIENGLMDPR LGVIDPSLRC 70 80 90 100 110 120 RTCGAKGGEC PGHFGSINLA RPVIHVGFAD TIHKILRSTC RKCGRVLLTE TEIEEYRQRI 130 140 150 160 170 180 LDAMEKEESL TPIIKEIYAE ARRDKCPHCE EEQEEIKLDK PVSIVEGDYK LTPSEVRERL 190 200 210 220 230 240 ERISDDDALI LGVNPEVARP EWMVLTVLPV PPVTVRPSIT LETGERSEDD LTHKLVDILR 250 260 270 280 290 300 INQRLKENME AGAPQLIVED LWELLQYHVT TYFDNEASGV PPARHRSGRP LKTLAQRLKG 310 320 330 340 350 360 KEGRFRSNLS GKRVNFSART VISPDPNISI NEVGVPEIIA REVTVPVYVT EWNIDRMREY 370 380 390 400 410 420 IENGPDVHPG ANYVIRPDGR KIRIYNETKE VVLENLKPGY IVERHLKDGD IVLFNRQPSL 430 440 450 460 470 480 HRMSMMAHQV RVLPYKTFRL NLCVCPPYNA DFDGDEMNMH VFQTEESRAE AKTLMRVQEH 490 500 510 520 530 540 ILSPRFGGPI IGGIHDHISG AYLLTRKSAV FSEEKVFQIL KKAGLPLPDS RGRDWTGKEI 550 560 570 580 590 600 FSMVLPDDLN MVYRAEVCRK CEECLEMECE NDAYVVIENG QLISGVIDEK AYGAFAGKIL 610 620 630 640 650 660 DHIVKEYGTD AAREFLDSAT KLAIAGIMHA GFTTSTNDEE IPEEARERIE AHLRNAEARV 670 680 690 700 710 720 DQLIEAYENG ELEPLPGRSL EETLEMKIMQ VLGEARDKSG EIAESYFDMD ENHAVIMALT 730 740 750 760 770 780 GARGSMLNLT QITACVGQQS VRGGRISRGY DNRTLPHFKK GELGAKSRGF VHSSYKEGLD 790 800 810 820 830 840 PIEFFFHAMG GREGLVDTAI RTAQSGYMQR RLVNALQDLT VDENGRVVDN RGVIIQNRFG 850 860 870 EDGVDPAKSD YGKIVDLDKL VEEIRLKSKG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85542.1.
PIR	G69006.
RefSeq	NP_276181.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27125.
SMR	O27125. Positions 6-864.
ModBase	Search...
Protein-protein interaction databases
STRING	O27125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471459.
GenomeReviews	Gene locus MTH_1051 in contig AE000666_GR.
KEGG	mth:MTH1051.
NMPDR	fig|187420.1.peg.1036.
Phylogenomic databases
eggNOG	arNOG04559.
HOGENOM	HBG499785.
OMA	HKLVDII.
PhylomeDB	O27125.
ProtClustDB	PRK08566.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1051-MONOMER.
Family and domain databases
InterPro	IPR012758. DNA-dir_RpoA1. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03041.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02390. RNA_pol_rpoA1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA1_METTH

Accession

Primary (citable) accession number: O27125

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program