O27124 (RPOB1_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit B'
EC=2.7.7.6

Gene names

Name:	rpoB1
Ordered Locus Names:	MTH_1050

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Subunit structure	M.thermoautotrophicum RNA polymerase is composed of four subunits: A',A'',B' and B''.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	DNA-directed RNA polymerase subunit B'		PRO_0000048103
Regions
	Zinc finger	546 – 567	22	Potential

Sequences

	Sequence		Length	Mass (Da)
	O27124 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 17F2C0FC70BD5BBC	FASTA	603	67,687
	10 20 30 40 50 60 MSKTKIYING KLIGTCENPE EFVEEMRKKR RSGEVSPEMN ITHYPENHEI YIFTDPGRAR 70 80 90 100 110 120 RPLIIVEDGE PLLTEEHLEK LETGEMSWDD LIAAGIIEYL DAEEEENAYI AMSPDEINDE 130 140 150 160 170 180 HTHLEIDPST MLGICAGIIP FANHNSSPRN TMEAGMTKQA LGLYASNYDL RTDTRAHLLH 190 200 210 220 230 240 HPQVPIVKTR IIDVTGYDER PSGQNFVVAV MSYEGYNMED ALILNKASLE RGLARSSFFR 250 260 270 280 290 300 SYEAAERRYP GGQEDRFEIP EKGVRGYRSE SDYRHLDEDG IINPEAEVSS GDVLIGKTSP 310 320 330 340 350 360 PRFLEEIDEF GTVAERRRET SVTVRHGEKG IVDAVLLTET VEGSRLAKIR VREQRQPELG 370 380 390 400 410 420 DKFASRHGQK GVVGLIVSQE DMPFTEDGIV PDLIVNPHAI PSRMSVGQVL EMLAGKAACM 430 440 450 460 470 480 EGRRVDGTPF TGEDEDDIKE ALRANGFETA GVESLYNGIT GERIEAEIFI GVAYYQKLHH 490 500 510 520 530 540 MTTDKVYARS RGPVQVLTRQ PTEGRAREGG LRFGEMERDC LIAHGAALAL KERLLDESDK 550 560 570 580 590 600 YEALVCADCG MIAVYDKIRD KKYCPICEDS ESFPVEVSYA FKLLLDELKS LCIFPKLILE DKA

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85541.1.
PIR	F69006.
RefSeq	NP_276180.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27124.
ModBase	Search...
Protein-protein interaction databases
STRING	O27124.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471458.
GenomeReviews	Gene locus MTH_1050 in contig AE000666_GR.
KEGG	mth:MTH1050.
NMPDR	fig|187420.1.peg.1035.
Phylogenomic databases
eggNOG	arNOG04560.
HOGENOM	HBG496836.
OMA	MSYEGYN.
PhylomeDB	O27124.
ProtClustDB	PRK07225.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1050-MONOMER.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR019969. DNA-dir_RNA_pol_su_B_arc. IPR007121. RNA_pol_bsu_CS. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KO	K03044.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR03670. RpoB_arch. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB1_METTH

Accession

Primary (citable) accession number: O27124

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program