O27123 (RPOB2_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit B''
EC=2.7.7.6

Gene names

Name:	rpoB2
Ordered Locus Names:	MTH_1049

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Subunit structure	M.thermoautotrophicum RNA polymerase is composed of four subunits: A',A'',B' and B''.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	DNA-directed RNA polymerase subunit B''		PRO_0000074032

Sequences

	Sequence		Length	Mass (Da)
	O27123 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F82AA2C85F6FC6D0	FASTA	519	59,191
	10 20 30 40 50 60 MFWRKSMKKS AWGLVDAFFD KYDLVDHHIH SYNDFVSNRI QEIIDTSEPI ELEQGKYRVE 70 80 90 100 110 120 TGKVSIEKPF IKEADGSKSK IYPTEARLRN LTYSAHMSLE MRLLKEGGPE TEFEKVYIGE 130 140 150 160 170 180 LPVMLKSEIC HLHGLSRKEL MEKGEDPADP GGYFIVNGSE RSIVTMEEIA PNKIILERIG 190 200 210 220 230 240 EEDENRARAI VTSIRSGFRA RISLEYRKPR KTGVFLRISF PYVPGEIPLV ILLRALGLAT 250 260 270 280 290 300 DQEIITSISD DFNYQMIAAD DIQVSLDRLN LNKKEMKELD EEDRREYLIR SAIKYIGNRV 310 320 330 340 350 360 AKGMTEDYRI KRAEDVIDRY LLPHIGTEPE KRLEKATYLA EMTEMLLQVI SGERRPHDKD 370 380 390 400 410 420 HYTNKRLRVS GDLMEDLLRV AFTSLSRDMS YQLERSLARG KEPSVKQAVR SDVLTENLKH 430 440 450 460 470 480 AIATGNWVGG RAGVSQLLDR TSYMGTLSHM RRVVSPLSRS QPHFEARDLH PTQFGKICPN 490 500 510 ETPEGPNCGL VKNLALLAKI SEGSDASEVE EVIKMGVVN

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85540.1.
PIR	D69006.
RefSeq	NP_276179.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27123.
ModBase	Search...
Protein-protein interaction databases
STRING	O27123.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471457.
GenomeReviews	Gene locus MTH_1049 in contig AE000666_GR.
KEGG	mth:MTH1049.
NMPDR	fig|187420.1.peg.1034.
Phylogenomic databases
eggNOG	arNOG04791.
HOGENOM	HBG496841.
OMA	LAGDLME.
ProtClustDB	PRK09606.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1049-MONOMER.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. [Graphical view]
KO	K03045.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB2_METTH

Accession

Primary (citable) accession number: O27123

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program