O27122 (RPOH_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit H
EC=2.7.7.6

Alternative name(s):
RPB5

Gene names

Name:	rpoH
Ordered Locus Names:	MTH_1048

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	77 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00025
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00025
Sequence similarities	Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 77

77

DNA-directed RNA polymerase subunit H HAMAP MF_00025

PRO_0000146096

Secondary structure

1

.

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.

.

77

Helix Strand Turn

Details...

Beta strand

7 – 10

4

Beta strand

13 – 17

5

Helix

19 – 28

10

Turn

33 – 35

3

Helix

44 – 47

4

Helix

48 – 50

3

Beta strand

56 – 63

8

Turn

64 – 66

3

Beta strand

67 – 74

8

Sequences

	Sequence		Length	Mass (Da)
	O27122 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 745435E11D2756DC	FASTA	77	8,808
	10 20 30 40 50 60 MKREILKHQL VPEHVILNES EAKRVLKELD AHPEQLPKIK TTDPVAKAIG AKRGDIVKII 70 RKSPTAEEFV TYRLVQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.
[2]	"Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum." Yee A., Booth V., Dharamsi A., Engel A., Edwards A.M., Arrowsmith C.H. Proc. Natl. Acad. Sci. U.S.A. 97:6311-6315(2000) [PubMed: 10841538] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000666 Genomic DNA. Translation: AAB85539.1.

PIR

C69006.

RefSeq

NP_276178.1. NC_000916.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EIK	NMR	-	A	1-77	[»]

ProteinModelPortal

O27122.

SMR

O27122. Positions 1-77.

ModBase

Search...

Protein-protein interaction databases

STRING

O27122.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1471456.

GenomeReviews

Gene locus MTH_1048 in contig AE000666_GR.

KEGG

mth:MTH1048.

NMPDR

fig|187420.1.peg.1033.

Phylogenomic databases

eggNOG

arNOG12569.

HOGENOM

HBG496846.

OMA

EHELVPK.

ProtClustDB

PRK09570.

Enzyme and pathway databases

BioCyc

MTHE187420:MTH1048-MONOMER.

Family and domain databases

HAMAP

MF_00025. RNApol_arch_H.
[Tree]

InterPro

IPR020609. DNA-dir_RNA_pol_subH/Rpb5_arc.
IPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
[Graphical view]

Gene3D

G3DSA:3.90.940.20. RNApol_RPB5. 1 hit.

KO

K03053.

Pfam

PF01191. RNA_pol_Rpb5_C. 1 hit.
[Graphical view]

ProDom

PD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF55287. RNApol_RPB5_like. 1 hit.

PROSITE

PS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPOH_METTH

Accession

Primary (citable) accession number: O27122

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program