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O27122 (RPOH_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit H

EC=2.7.7.6
Alternative name(s):
RPB5
Gene names
Name:rpoH
Ordered Locus Names:MTH_1048
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length77 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_00025

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00025

Sequence similarities

Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7777DNA-directed RNA polymerase subunit H HAMAP-Rule MF_00025
PRO_0000146096

Secondary structure

................ 77
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O27122 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 745435E11D2756DC

FASTA778,808
        10         20         30         40         50         60 
MKREILKHQL VPEHVILNES EAKRVLKELD AHPEQLPKIK TTDPVAKAIG AKRGDIVKII 

        70 
RKSPTAEEFV TYRLVQD 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[2]"Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum."
Yee A., Booth V., Dharamsi A., Engel A., Edwards A.M., Arrowsmith C.H.
Proc. Natl. Acad. Sci. U.S.A. 97:6311-6315(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85539.1.
PIRC69006.
RefSeqNP_276178.1. NC_000916.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EIKNMR-A1-77[»]
ProteinModelPortalO27122.
SMRO27122. Positions 1-77.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH1048.

Protocols and materials databases

DNASU1471456.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85539; AAB85539; MTH_1048.
GeneID1471456.
KEGGmth:MTH1048.

Phylogenomic databases

eggNOGCOG2012.
KOK03053.
OMAEHELVPK.
ProtClustDBPRK09570.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-1050-MONOMER.

Family and domain databases

Gene3D3.90.940.20. 1 hit.
HAMAPMF_00025. RNApol_RpoH_RPB5.
InterProIPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
IPR020609. RpoH/RPB5.
[Graphical view]
PfamPF01191. RNA_pol_Rpb5_C. 1 hit.
[Graphical view]
ProDomPD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55287. SSF55287. 1 hit.
PROSITEPS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO27122.

Entry information

Entry nameRPOH_METTH
AccessionPrimary (citable) accession number: O27122
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references