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Protein

DNA-directed RNA polymerase subunit H

Gene

rpoH

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit HUniRule annotation (EC:2.7.7.6UniRule annotation)
Alternative name(s):
RPB5
Gene namesi
Name:rpoHUniRule annotation
Ordered Locus Names:MTH_1048
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7777DNA-directed RNA polymerase subunit HPRO_0000146096Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1048.

Structurei

Secondary structure

1
77
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104Combined sources
Beta strandi13 – 175Combined sources
Helixi19 – 2810Combined sources
Turni33 – 353Combined sources
Helixi44 – 474Combined sources
Helixi48 – 503Combined sources
Beta strandi56 – 638Combined sources
Turni64 – 663Combined sources
Beta strandi67 – 748Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EIKNMR-A1-77[»]
ProteinModelPortaliO27122.
SMRiO27122. Positions 1-77.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO27122.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2012.
KOiK03053.
OMAiEHELVPK.

Family and domain databases

Gene3Di3.90.940.20. 1 hit.
HAMAPiMF_00025. RNApol_RpoH_RPB5.
InterProiIPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
IPR020609. RpoH/RPB5.
[Graphical view]
PfamiPF01191. RNA_pol_Rpb5_C. 1 hit.
[Graphical view]
ProDomiPD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55287. SSF55287. 1 hit.
PROSITEiPS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27122-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKREILKHQL VPEHVILNES EAKRVLKELD AHPEQLPKIK TTDPVAKAIG
60 70
AKRGDIVKII RKSPTAEEFV TYRLVQD
Length:77
Mass (Da):8,808
Last modified:January 1, 1998 - v1
Checksum:i745435E11D2756DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85539.1.
PIRiC69006.
RefSeqiNP_276178.1. NC_000916.1.
WP_010876674.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85539; AAB85539; MTH_1048.
GeneIDi1471456.
KEGGimth:MTH1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85539.1.
PIRiC69006.
RefSeqiNP_276178.1. NC_000916.1.
WP_010876674.1. NC_000916.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EIKNMR-A1-77[»]
ProteinModelPortaliO27122.
SMRiO27122. Positions 1-77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1048.

Protocols and materials databases

DNASUi1471456.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85539; AAB85539; MTH_1048.
GeneIDi1471456.
KEGGimth:MTH1048.

Phylogenomic databases

eggNOGiCOG2012.
KOiK03053.
OMAiEHELVPK.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1050-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO27122.

Family and domain databases

Gene3Di3.90.940.20. 1 hit.
HAMAPiMF_00025. RNApol_RpoH_RPB5.
InterProiIPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
IPR020609. RpoH/RPB5.
[Graphical view]
PfamiPF01191. RNA_pol_Rpb5_C. 1 hit.
[Graphical view]
ProDomiPD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55287. SSF55287. 1 hit.
PROSITEiPS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum."
    Yee A., Booth V., Dharamsi A., Engel A., Edwards A.M., Arrowsmith C.H.
    Proc. Natl. Acad. Sci. U.S.A. 97:6311-6315(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiRPOH_METTH
AccessioniPrimary (citable) accession number: O27122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 4, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.