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O27093 (HEM1_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-tRNA reductase
Short name=GluTR
EC=1.2.1.70
Gene names
Name:hemA
Ordered Locus Names:MTH_1012
OrganismMethanobacterium thermoautotrophicum (strain Delta H)
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. HAMAP MF_00087

Catalytic activity

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. HAMAP MF_00087

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. HAMAP MF_00087

Subunit structure

Homodimer By similarity. HAMAP MF_00087

Domain

Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization By similarity. HAMAP MF_00087

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. HAMAP MF_00087

Sequence similarities

Belongs to the glutamyl-tRNA reductase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processporphyrin-containing compound biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionNADP binding

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 402402Glutamyl-tRNA reductase HAMAP MF_00087
PRO_0000114105

Regions

Nucleotide binding171 – 1766NADP By similarity
Region48 – 514Substrate binding By similarity
Region96 – 983Substrate binding By similarity

Sites

Active site491Nucleophile By similarity
Binding site911Substrate By similarity
Binding site1021Substrate By similarity
Site811Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O27093 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 40063562D2E24978

FASTA40244,941
        10         20         30         40         50         60 
MLQVILNIRL DHKTSDVKTM ESSHERMEAI VAELESSGTV MEKVPIRTCN RIEYYLSVQE 

        70         80         90        100        110        120 
IPPGFEFDGF TVEGDEDALR HILRLASGLE SMIIGEDQIL GQIKAARVQA LREGTCGPVL 

       130        140        150        160        170        180 
DMVFTKAVHV GQTVRRKTQI NRGSVSIGSA AVDLAESIHG DLKCRKVLAI GAGKMGTLVA 

       190        200        210        220        230        240 
RALAEKHLSA IMVANRTYER AYQLACELGG DAIHFDRLNR ALRDADVVIS ATGSPHYILT 

       250        260        270        280        290        300 
RERVRDAIPP ERRSAVVMVD IANPRDIEES VRELGIRLFT IDDLRGVAEE NRRRREAEAR 

       310        320        330        340        350        360 
EAERIVEGEL KLLLRSLKHM EVEPLLAEVR GNMESLRRRE AERALNKIMN SSDPEGVIEA 

       370        380        390        400 
LSRSIVDKIF HDIAISIRQA AERGDEEFLS MCAKLFNCKD LK

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Delta H.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000666 Genomic DNA. Translation: AAB85508.1.
PIRA69002.
RefSeqNP_276147.1. NC_000916.1.

3D structure databases

ProteinModelPortalO27093.
ModBaseSearch...

Protein-protein interaction databases

STRINGO27093.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1471420.
GenomeReviewsGene locus MTH_1012 in contig AE000666_GR.
KEGGmth:MTH1012.
NMPDRfig|187420.1.peg.1002.

Phylogenomic databases

eggNOGarNOG04783.
HOGENOMHBG732626.
OMAYADIVIS.
PhylomeDBO27093.
ProtClustDBPRK00045.

Enzyme and pathway databases

BioCycMTHE187420:MTH1012-MONOMER.

Family and domain databases

HAMAPMF_00087. Glu-tRNA_reductase.
[Tree]
InterProIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR016040. NAD(P)-bd_dom.
IPR018214. Pyrrol_synth_GluRdtase_CS.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK02492.
PfamPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
PIRSFPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMSSF69075. 4pyrrol_synth_GluRdtase_C. 1 hit.
SSF69742. GlutR. 1 hit.
TIGRFAMsTIGR01035. HemA. 1 hit.
PROSITEPS00747. GLUTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM1_METTH
AccessionPrimary (citable) accession number: O27093
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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