ID   G3P_METTH               Reviewed;         337 AA.
AC   O27090;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 71.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE            Short=GAPDH;
DE            EC=1.2.1.59;
DE   AltName: Full=NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase;
GN   Name=gap; OrderedLocusNames=MTH_1009;
OS   Methanobacterium thermoautotrophicum.
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=98037514; PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; AE000666; AAB85505.1; -; Genomic_DNA.
DR   PIR; E69001; E69001.
DR   RefSeq; NP_276144.1; -.
DR   HSSP; P10618; 1CF2.
DR   SMR; O27090; 1-337.
DR   GeneID; 1471417; -.
DR   GenomeReviews; AE000666_GR; MTH_1009.
DR   KEGG; mth:MTH1009; -.
DR   NMPDR; fig|187420.1.peg.999; -.
DR   HOGENOM; O27090; -.
DR   OMA; O27090; AIFQGGE.
DR   BioCyc; MTHE187420:MTH1009-MON; -.
DR   BRENDA; 1.2.1.59; 270.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043891; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:HAMAP.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00559; -; 1.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006436; Glyceraldehyde-3-P_DH_2_arc.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   ProDom; PD004105; DapB; 1.
DR   ProDom; PD007761; GAPDH_like; 1.
DR   TIGRFAMs; TIGR01546; GAPDH-II_archae; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    337       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000145726.
FT   NP_BIND      11     12       NAD (By similarity).
FT   REGION      139    141       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      194    195       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    140    140       Nucleophile (By similarity).
FT   BINDING     110    110       NAD; via amide nitrogen (By similarity).
FT   BINDING     168    168       NAD (By similarity).
FT   BINDING     301    301       NAD; via carbonyl oxygen (By similarity).
SQ   SEQUENCE   337 AA;  36836 MW;  730FEDB11CF9BAA3 CRC64;
     MISVAINGYG TIGKRVADAV AAQDDMKVAG VSKTKPDFEA RVAIEKGYDL YVSIPEREKL
     FGEAGIPVSG TVEDMLEEAD IVVDATPEGI GAKNLEMYRE KGIKAIFQGG EKHDAIGLSF
     NSFANYDESL GADYTRVVSC NTTGLCRTLK PIDDLCGIKK VRAVMVRRGA DPVQVKKGPI
     NAIVPNPPTV PSHHGPDLKT VMKGVNIHTV ALLVPTTLMH QHNIMVELED PVEADEIKAR
     LDETTRVMLV RASEGLASTA EIMEYAKELG RSRNDLFEIP VWEESINVVD GELFYMQAVH
     QESDAVPESV DAIRALLELE EDNMKSIMKT NRAMGIL
//