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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NAD; via amide nitrogenBy similarity1
Active sitei140NucleophileBy similarity1
Binding sitei168NADBy similarity1
Binding sitei301NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:MTH_1009
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457261 – 337Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST337

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi187420.MTH1009.

Structurei

3D structure databases

ProteinModelPortaliO27090.
SMRiO27090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

O27090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISVAINGYG TIGKRVADAV AAQDDMKVAG VSKTKPDFEA RVAIEKGYDL
60 70 80 90 100
YVSIPEREKL FGEAGIPVSG TVEDMLEEAD IVVDATPEGI GAKNLEMYRE
110 120 130 140 150
KGIKAIFQGG EKHDAIGLSF NSFANYDESL GADYTRVVSC NTTGLCRTLK
160 170 180 190 200
PIDDLCGIKK VRAVMVRRGA DPVQVKKGPI NAIVPNPPTV PSHHGPDLKT
210 220 230 240 250
VMKGVNIHTV ALLVPTTLMH QHNIMVELED PVEADEIKAR LDETTRVMLV
260 270 280 290 300
RASEGLASTA EIMEYAKELG RSRNDLFEIP VWEESINVVD GELFYMQAVH
310 320 330
QESDAVPESV DAIRALLELE EDNMKSIMKT NRAMGIL
Length:337
Mass (Da):36,836
Last modified:January 1, 1998 - v1
Checksum:i730FEDB11CF9BAA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85505.1.
PIRiE69001.
RefSeqiWP_010876640.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85505; AAB85505; MTH_1009.
GeneIDi1471417.
KEGGimth:MTH_1009.
PATRICifig|187420.15.peg.992.

Similar proteinsi

Entry informationi

Entry nameiG3P_METTH
AccessioniPrimary (citable) accession number: O27090
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families