O27089 (TOP6A_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type 2 DNA topoisomerase 6 subunit A
EC=5.99.1.3

Alternative name(s):
Type II DNA topoisomerase VI subunit A

Gene names

Name:	top6A
Ordered Locus Names:	MTH_1008

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Relaxes both positive and negative superturns and exhibits a strong decatenase activity By similarity. HAMAP-Rule MF_00132
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00132
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer. Heterotetramer of two Top6A and two Top6B chains By similarity.
Sequence similarities	Belongs to the TOP6A family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Type 2 DNA topoisomerase 6 subunit A HAMAP-Rule MF_00132		PRO_0000145451
Sites
	Active site	96	1	Nucleophile By similarity
	Metal binding	186	1	Magnesium By similarity
	Metal binding	238	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27089 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7225C957AC36BC0E	FASTA	353	40,029
	10 20 30 40 50 60 MNRREIAINK LKSLGDVILD DVTQGRIPRI KVPSRGTSNI IYDEDKRHYV LGDRYGTRSM 70 80 90 100 110 120 GNVKQIKKIG QMLYTANFCK DLVAREKTAT LRELYYISEG WEVDFADQQE SNIVGEDLEV 130 140 150 160 170 180 TLGMTREELG LMPEEDGASV YGALTVREGD IEIDALRSGK SGYNISPTID EVEFVDHDVE 190 200 210 220 230 240 RVIAVETMGM FHRLVQEKAY KKFDALIVGL KGQAARATRR FIKRVNEELN LPVYICNDGD 250 260 270 280 290 300 PWGFHIAMVI ISGSAKLAHV NHQLATPDAK FLGVTASDII NYDLPTDPLK DVDVVRLKEL 310 320 330 340 350 LQDPRYRGDF WKTEIKKMLT IGKKAEQQSF SKYGLEYVVD TYLPEKLEAV ENQ

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85504.1.
PIR	D69001.
RefSeq	NP_276143.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27089.
ModBase	Search...
Protein-protein interaction databases
STRING	187420.MTH1008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB85504; AAB85504; MTH_1008.
GeneID	1471416.
KEGG	mth:MTH1008.
Phylogenomic databases
eggNOG	COG1697.
KO	K03166.
OMA	CTTRKIK.
ProtClustDB	PRK04342.
Enzyme and pathway databases
BioCyc	MTHE187420:GJNM-1010-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00132. Top6A.
InterPro	IPR002815. Spo11/TopoVI_A. IPR013049. Spo11/TopoVI_A_N. IPR004085. TopoVI_A. IPR022904. TopoVI_suA_arc. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR10848. PTHR10848. 1 hit.
Pfam	PF04406. TP6A_N. 1 hit. [Graphical view]
PRINTS	PR01550. TOP6AFAMILY. PR01552. TPISMRASE6A.
SUPFAM	SSF56726. Spo11/TopoVI_A. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TOP6A_METTH

Accession

Primary (citable) accession number: O27089

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program