O27079 (CARA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	MTH_998

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.
Sequence caution	The sequence AAB85495.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112361
Regions
	Domain	176 – 356	181	Glutamine amidotransferase type-1
	Region	1 – 177	177	CPSase HAMAP MF_01209
Sites
	Active site	251	1	Nucleophile By similarity
	Active site	336	1	By similarity
	Active site	338	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27079 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 2222F16A3429FFF7	FASTA	356	38,958
	10 20 30 40 50 60 MFKEAKLALE DGTIIRGEAF GSETVKTGEV VFATGMTGYV ESLTDPSYRG QILMPTYPLQ 70 80 90 100 110 120 GNYGVTEEWY QSDGIKAEGL IVREMCPAPS HSLSEKTLPE FLEEYGIPGI AGVDTRALTI 130 140 150 160 170 180 KIRDKGTMKG ALATEEIDDD ELLEMAVKQP DITEIDLVDM VCVREPVILN EDASGRVVIV 190 200 210 220 230 240 DCGIKRNSID ALLEKDLGVV IVPYTTSPAE IMDYEPDGLL ISSGPGDPTR VRDAIDTVKA 250 260 270 280 290 300 LSERLPIFGI CLGQQIIALA FGARIYKMKF GHRGVNQPVK DLRSGTVSIT SQNHGFTVDP 310 320 330 340 350 DSVKGTDIEI TQLNLNDGTP EGIEHRELPV FSVQYHPEAG PGPHDTVNIF DRLWMF

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85495.1. Different initiation.
PIR	F69233.
RefSeq	NP_276133.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27079.
ModBase	Search...
Protein-protein interaction databases
STRING	O27079.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471406.
GenomeReviews	Gene locus MTH_998 in contig AE000666_GR.
KEGG	mth:MTH998.
NMPDR	fig|187420.1.peg.988.
Phylogenomic databases
eggNOG	arNOG04623.
HOGENOM	HBG286341.
OMA	YESIAPT.
PhylomeDB	O27079.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH998-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_METTH

Accession

Primary (citable) accession number: O27079

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	July 11, 2002
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program