Reviewed, UniProtKB/Swiss-Prot O27054 (ASPD_METTH)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	MTH_973

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_0000144900
Sites
	Active site	208	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	180	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27054-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D9A387A535611FC7	FASTA	257	27,569
	10 20 30 40 50 60 MILMMVGIVG CGAIANLITG FVRDGRVPVK LGFFYDRDLE RAENLASMVD GRAVLDVADM 70 80 90 100 110 120 LPEVDLVVEA ASPEAVRDLV PEILEAGKDV VVMSVGALMD PELREMLVEL ASLNDATIHV 130 140 150 160 170 180 PSGAIVGLDG LKAASMGNIE SVKLITRKPP RSLGISMDEK KVLYRGRASE AVKKFPLNIN 190 200 210 220 230 240 VAAALSLACD RDVDVEIIAD PAVDRNVHEV TVRGDFGEFK TITENVRCSV NPKTSVMAAY 250 SAIKLLKSLS ENIHIGT

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85469.1.
PIR	C69230.
RefSeq	NP_276108.1.
3D structure databases
HSSP	HSSP built from PDB template 1J5P based on UniProtKB Q9X1X6.
ModBase	Search...
Protein-protein interaction databases
STRING	O27054.
Genome annotation databases
GeneID	1471381.
GenomeReviews	Gene locus MTH_973 in contig AE000666_GR.
KEGG	mth:MTH973.
NMPDR	fig|187420.1.peg.963.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27054.
OMA	FPANLNV.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH973-MON.
BRENDA	1.4.1.21. 270.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_METTH

Accession

Primary (citable) accession number: O27054

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)