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O27054 (ASPD_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP-Rule MF_01265

Catalytic activity

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP-Rule MF_01265

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP-Rule MF_01265

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.

Sequence similarities

Belongs to the L-aspartate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Probable L-aspartate dehydrogenase HAMAP-Rule MF_01265
PRO_0000144900

Sites

Active site2081 By similarity
Binding site1241NAD; via amide nitrogen By similarity
Binding site1801NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O27054 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: D9A387A535611FC7

FASTA25727,569
        10         20         30         40         50         60 
MILMMVGIVG CGAIANLITG FVRDGRVPVK LGFFYDRDLE RAENLASMVD GRAVLDVADM 

        70         80         90        100        110        120 
LPEVDLVVEA ASPEAVRDLV PEILEAGKDV VVMSVGALMD PELREMLVEL ASLNDATIHV 

       130        140        150        160        170        180 
PSGAIVGLDG LKAASMGNIE SVKLITRKPP RSLGISMDEK KVLYRGRASE AVKKFPLNIN 

       190        200        210        220        230        240 
VAAALSLACD RDVDVEIIAD PAVDRNVHEV TVRGDFGEFK TITENVRCSV NPKTSVMAAY 

       250 
SAIKLLKSLS ENIHIGT 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85469.1.
PIRC69230.
RefSeqNP_276108.1. NC_000916.1.

3D structure databases

ProteinModelPortalO27054.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH973.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85469; AAB85469; MTH_973.
GeneID1471381.
KEGGmth:MTH973.

Phylogenomic databases

eggNOGCOG1712.
KOK06989.
OMAECAGHSA.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-975-MONOMER.
UniPathwayUPA00253; UER00456.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01265. NadX.
InterProIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR011182. Asp_DH_NAD_syn.
IPR020626. Asp_DH_NAD_syn_prok.
IPR022487. Asp_DH_NAD_synth_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsTIGR03855. NAD_NadX. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASPD_METTH
AccessionPrimary (citable) accession number: O27054
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways