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O27054

- ASPD_METTH

UniProt

O27054 - ASPD_METTH

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Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

Catalytic activityi

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241NAD; via amide nitrogenUniRule annotation
Binding sitei180 – 1801NADUniRule annotation
Active sitei208 – 2081UniRule annotation

GO - Molecular functioni

  1. aspartate dehydrogenase activity Source: UniProtKB-EC
  2. NAD binding Source: UniProtKB-HAMAP
  3. NADP binding Source: UniProtKB-HAMAP
  4. oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD biosynthetic process Source: UniProtKB-HAMAP
  2. NADP catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-975-MONOMER.
UniPathwayiUPA00253; UER00456.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
Gene namesi
Name:nadXUniRule annotation
Ordered Locus Names:MTH_973
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Probable L-aspartate dehydrogenasePRO_0000144900Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH973.

Structurei

3D structure databases

ProteinModelPortaliO27054.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-aspartate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1712.
KOiK06989.
OMAiECAGHSA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01265. NadX.
InterProiIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.

Sequencei

Sequence statusi: Complete.

O27054-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILMMVGIVG CGAIANLITG FVRDGRVPVK LGFFYDRDLE RAENLASMVD
60 70 80 90 100
GRAVLDVADM LPEVDLVVEA ASPEAVRDLV PEILEAGKDV VVMSVGALMD
110 120 130 140 150
PELREMLVEL ASLNDATIHV PSGAIVGLDG LKAASMGNIE SVKLITRKPP
160 170 180 190 200
RSLGISMDEK KVLYRGRASE AVKKFPLNIN VAAALSLACD RDVDVEIIAD
210 220 230 240 250
PAVDRNVHEV TVRGDFGEFK TITENVRCSV NPKTSVMAAY SAIKLLKSLS

ENIHIGT
Length:257
Mass (Da):27,569
Last modified:January 1, 1998 - v1
Checksum:iD9A387A535611FC7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85469.1.
PIRiC69230.
RefSeqiNP_276108.1. NC_000916.1.
WP_010876604.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85469; AAB85469; MTH_973.
GeneIDi1471381.
KEGGimth:MTH973.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85469.1 .
PIRi C69230.
RefSeqi NP_276108.1. NC_000916.1.
WP_010876604.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27054.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH973.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85469 ; AAB85469 ; MTH_973 .
GeneIDi 1471381.
KEGGi mth:MTH973.

Phylogenomic databases

eggNOGi COG1712.
KOi K06989.
OMAi ECAGHSA.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00456 .
BioCyci MTHE187420:GJNM-975-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_01265. NadX.
InterProi IPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR022487. Asp_DH_arc.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsi TIGR03855. NAD_NadX. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiASPD_METTH
AccessioniPrimary (citable) accession number: O27054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3