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O27054

- ASPD_METTH

UniProt

O27054 - ASPD_METTH

Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

    Catalytic activityi

    L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei124 – 1241NAD; via amide nitrogenUniRule annotation
    Binding sitei180 – 1801NADUniRule annotation
    Active sitei208 – 2081UniRule annotation

    GO - Molecular functioni

    1. aspartate dehydrogenase activity Source: UniProtKB-EC
    2. NAD binding Source: UniProtKB-HAMAP
    3. NADP binding Source: UniProtKB-HAMAP
    4. oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD biosynthetic process Source: UniProtKB-HAMAP
    2. NADP catabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-975-MONOMER.
    UniPathwayiUPA00253; UER00456.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
    Gene namesi
    Name:nadXUniRule annotation
    Ordered Locus Names:MTH_973
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257Probable L-aspartate dehydrogenasePRO_0000144900Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi187420.MTH973.

    Structurei

    3D structure databases

    ProteinModelPortaliO27054.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the L-aspartate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1712.
    KOiK06989.
    OMAiECAGHSA.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_01265. NadX.
    InterProiIPR005106. Asp/hSer_DH_NAD-bd.
    IPR002811. Asp_DH.
    IPR022487. Asp_DH_arc.
    IPR020626. Asp_DH_prok.
    IPR011182. L-Asp_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01958. DUF108. 1 hit.
    PF03447. NAD_binding_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
    TIGRFAMsiTIGR03855. NAD_NadX. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O27054-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILMMVGIVG CGAIANLITG FVRDGRVPVK LGFFYDRDLE RAENLASMVD    50
    GRAVLDVADM LPEVDLVVEA ASPEAVRDLV PEILEAGKDV VVMSVGALMD 100
    PELREMLVEL ASLNDATIHV PSGAIVGLDG LKAASMGNIE SVKLITRKPP 150
    RSLGISMDEK KVLYRGRASE AVKKFPLNIN VAAALSLACD RDVDVEIIAD 200
    PAVDRNVHEV TVRGDFGEFK TITENVRCSV NPKTSVMAAY SAIKLLKSLS 250
    ENIHIGT 257
    Length:257
    Mass (Da):27,569
    Last modified:January 1, 1998 - v1
    Checksum:iD9A387A535611FC7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85469.1.
    PIRiC69230.
    RefSeqiNP_276108.1. NC_000916.1.
    WP_010876604.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85469; AAB85469; MTH_973.
    GeneIDi1471381.
    KEGGimth:MTH973.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85469.1 .
    PIRi C69230.
    RefSeqi NP_276108.1. NC_000916.1.
    WP_010876604.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O27054.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85469 ; AAB85469 ; MTH_973 .
    GeneIDi 1471381.
    KEGGi mth:MTH973.

    Phylogenomic databases

    eggNOGi COG1712.
    KOi K06989.
    OMAi ECAGHSA.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00456 .
    BioCyci MTHE187420:GJNM-975-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_01265. NadX.
    InterProi IPR005106. Asp/hSer_DH_NAD-bd.
    IPR002811. Asp_DH.
    IPR022487. Asp_DH_arc.
    IPR020626. Asp_DH_prok.
    IPR011182. L-Asp_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01958. DUF108. 1 hit.
    PF03447. NAD_binding_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005227. Asp_dh_NAD_syn. 1 hit.
    TIGRFAMsi TIGR03855. NAD_NadX. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiASPD_METTH
    AccessioniPrimary (citable) accession number: O27054
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3