O27051 (SERA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-3-phosphoglycerate dehydrogenase
Short name=PGDH
EC=1.1.1.95

Gene names

Name:	serA
Ordered Locus Names:	MTH_970

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	D-3-phosphoglycerate dehydrogenase		PRO_0000076010
Regions
	Domain	452 – 523	72	ACT
	Nucleotide binding	151 – 152	2	NAD By similarity
	Nucleotide binding	230 – 232	3	NAD By similarity
	Nucleotide binding	279 – 282	4	NAD By similarity
Sites
	Active site	232	1	By similarity
	Active site	261	1	By similarity
	Active site	279	1	Proton donor By similarity
	Binding site	171	1	NAD By similarity
	Binding site	256	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27051 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DDD63FCFF812B1C5	FASTA	525	56,991
	10 20 30 40 50 60 MSRMKVLIAD SINEKGISEL EEVAEVVVNT TITPEELLDA IKDFDAIVVR SRTKVTREVI 70 80 90 100 110 120 EAAPRLKIIA RAGVGVDNVD VKAATDRGIM VINAPESTSI TVAEHSIGLM LALARKIAIA 130 140 150 160 170 180 DRSVKEGKWE KNRFMGIELN GKTLGIIGMG RIGSQVVVRT KAFGMDIMVY DPYISKEAAE 190 200 210 220 230 240 EMGVTVTDLE TLLRESDIVT IHVPLTPETR HLISEDEFKL MKDTAFIVNC ARGGIIDEDA 250 260 270 280 290 300 LYRALKDGEI AGAALDVFEE EPPEGSPLLE LENVVLTPHI GASTSEAQRD AAIIVANEIK 310 320 330 340 350 360 TVFQGGAPRN VLNMPVMDSE TYKSLKPYIE LAEKMGAIIA QALPGNIEKL DVTYCGELAE 370 380 390 400 410 420 MQFDILTRTM LQAILNPILT EPVNLINAPS IAKKRGIMVT EARRSESDGY RSIIIATAES 430 440 450 460 470 480 DRGGFSVEAT HIKEPTIIGI NGYRVDVKPE GTMIIARYRD LPGTIGAIGT KLGQHGINIA 490 500 510 520 TMQVGRKEIG GEAVMVLKVD QSVPAEVIEE VKKLDNVDDA VAIEI

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85466.1.
PIR	H69229.
RefSeq	NP_276105.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27051.
SMR	O27051. Positions 4-305.
ModBase	Search...
Protein-protein interaction databases
STRING	O27051.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471378.
GenomeReviews	Gene locus MTH_970 in contig AE000666_GR.
KEGG	mth:MTH970.
NMPDR	fig|187420.1.peg.960.
Phylogenomic databases
eggNOG	arNOG04496.
HOGENOM	HBG731446.
OMA	AEHTVAM.
PhylomeDB	O27051.
ProtClustDB	PRK13581.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH970-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00058.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_METTH

Accession

Primary (citable) accession number: O27051

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program