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O27026 (CAPPA_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppcA
Ordered Locus Names:MTH_943
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Ref.2

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. Ref.2

Cofactor

Magnesium. Ref.2

Enzyme regulation

Inhibited by NaCl, KCl, ATP, ADP, GTP and aspartate. Unlike E.coli, not regulated by acetyl-CoA. Ref.2

Subunit structure

Homotetramer. Ref.2

Sequence similarities

Belongs to the PEPCase type 2 family.

Biophysicochemical properties

Kinetic parameters:

KM=1.8 mM for KHCO3 Ref.2

KM=0.46 mM for phosphoenolpyruvate

pH dependence:

Optimum pH is 6.8.

Temperature dependence:

Optimum temperature is 62 degrees Celsius.

Sequence caution

The sequence AAB85441.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904
PRO_0000309600

Sequences

Sequence LengthMass (Da)Tools
O27026 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: DA053C2E9C7F565A

FASTA48355,078
        10         20         30         40         50         60 
MKVPRCMSTQ HPDNVNPPFF AEEPELGGED EIREAYYVFS HLGCDEQMWD CEGKEVDNYV 

        70         80         90        100        110        120 
VKKLLTKYQA FFRDHVLGED LRLTLRVPNP TVERAEAKIL LETLESIPRS YDTASLFYGM 

       130        140        150        160        170        180 
DAAPVFEVIL PMTSSSSCLN RIHSYYLDFV KGKERLQLAD GVTVKEWIGE FRPDEINVIP 

       190        200        210        220        230        240 
LFEDHEGMLN AAKITGEYLD GKDIQEQRVF LARSDPAMNY GMISATLLNR IALSDFRDLE 

       250        260        270        280        290        300 
EESGVKLYPI IGMGSAPFRG NLRPDNVEDV TWEYRGAYTF TVQSSFKYDH EPSDVIRGIK 

       310        320        330        340        350        360 
KLRSVKPGRA AEIERESVLE IISAYCREYR RQVMDLVDII NRVARYVPGR RKRKLHIGLF 

       370        380        390        400        410        420 
GYSRSMGNVS LPRAITFTAA LYSLGVPPEL LGFNALSSGD LEFIEEVYPG LGRDLHDAAR 

       430        440        450        460        470        480 
YANPESPFLS PEVKSSFEEY LEPEYDEGHM KTTEEIIRAL RINRTANLQE LILEAASQRK 


FLG 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[2]"The phosphoenolpyruvate carboxylase from Methanothermobacter thermautotrophicus has a novel structure."
Patel H.M., Kraszewski J.L., Mukhopadhyay B.
J. Bacteriol. 186:5129-5137(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85441.1. Different initiation.
PIRD69226.
RefSeqNP_276080.1. NC_000916.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH943.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85441; AAB85441; MTH_943.
GeneID1471351.
KEGGmth:MTH943.

Phylogenomic databases

eggNOGCOG1892.
KOK01595.
OMADEYMPDY.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14528.
MTHE187420:GJNM-945-MONOMER.
SABIO-RKO27026.

Family and domain databases

HAMAPMF_01904. PEPcase_type2.
InterProIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFPIRSF006677. UCP006677. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR02751. PEPCase_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAPPA_METTH
AccessionPrimary (citable) accession number: O27026
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families