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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by NaCl, KCl, ATP, ADP, GTP and aspartate. Unlike E.coli, not regulated by acetyl-CoA.1 Publication

Kineticsi

  1. KM=1.8 mM for KHCO31 Publication
  2. KM=0.46 mM for phosphoenolpyruvate1 Publication

    pH dependencei

    Optimum pH is 6.8.1 Publication

    Temperature dependencei

    Optimum temperature is 62 degrees Celsius.1 Publication

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • phosphoenolpyruvate carboxylase activity Source: UniProtKB

    GO - Biological processi

    • carbon fixation Source: UniProtKB
    • oxaloacetate metabolic process Source: UniProtKB
    • tricarboxylic acid cycle Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14528.
    MTHE187420:GJNM-945-MONOMER.
    SABIO-RKO27026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase (EC:4.1.1.31)
    Short name:
    PEPC
    Short name:
    PEPCase
    Gene namesi
    Name:ppcA
    Ordered Locus Names:MTH_943
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Phosphoenolpyruvate carboxylasePRO_0000309600Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi187420.MTH943.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG1892.
    KOiK01595.
    OMAiQSAFRYD.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O27026-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKVPRCMSTQ HPDNVNPPFF AEEPELGGED EIREAYYVFS HLGCDEQMWD
    60 70 80 90 100
    CEGKEVDNYV VKKLLTKYQA FFRDHVLGED LRLTLRVPNP TVERAEAKIL
    110 120 130 140 150
    LETLESIPRS YDTASLFYGM DAAPVFEVIL PMTSSSSCLN RIHSYYLDFV
    160 170 180 190 200
    KGKERLQLAD GVTVKEWIGE FRPDEINVIP LFEDHEGMLN AAKITGEYLD
    210 220 230 240 250
    GKDIQEQRVF LARSDPAMNY GMISATLLNR IALSDFRDLE EESGVKLYPI
    260 270 280 290 300
    IGMGSAPFRG NLRPDNVEDV TWEYRGAYTF TVQSSFKYDH EPSDVIRGIK
    310 320 330 340 350
    KLRSVKPGRA AEIERESVLE IISAYCREYR RQVMDLVDII NRVARYVPGR
    360 370 380 390 400
    RKRKLHIGLF GYSRSMGNVS LPRAITFTAA LYSLGVPPEL LGFNALSSGD
    410 420 430 440 450
    LEFIEEVYPG LGRDLHDAAR YANPESPFLS PEVKSSFEEY LEPEYDEGHM
    460 470 480
    KTTEEIIRAL RINRTANLQE LILEAASQRK FLG
    Length:483
    Mass (Da):55,078
    Last modified:November 13, 2007 - v2
    Checksum:iDA053C2E9C7F565A
    GO

    Sequence cautioni

    The sequence AAB85441.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85441.1. Different initiation.
    PIRiD69226.
    RefSeqiNP_276080.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85441; AAB85441; MTH_943.
    GeneIDi1471351.
    KEGGimth:MTH943.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85441.1. Different initiation.
    PIRiD69226.
    RefSeqiNP_276080.1. NC_000916.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi187420.MTH943.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAB85441; AAB85441; MTH_943.
    GeneIDi1471351.
    KEGGimth:MTH943.

    Phylogenomic databases

    eggNOGiCOG1892.
    KOiK01595.
    OMAiQSAFRYD.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14528.
    MTHE187420:GJNM-945-MONOMER.
    SABIO-RKO27026.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. "The phosphoenolpyruvate carboxylase from Methanothermobacter thermautotrophicus has a novel structure."
      Patel H.M., Kraszewski J.L., Mukhopadhyay B.
      J. Bacteriol. 186:5129-5137(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiCAPPA_METTH
    AccessioniPrimary (citable) accession number: O27026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: November 13, 2007
    Last modified: July 22, 2015
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.