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O27026

- CAPPA_METTH

UniProt

O27026 - CAPPA_METTH

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Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by NaCl, KCl, ATP, ADP, GTP and aspartate. Unlike E.coli, not regulated by acetyl-CoA.1 Publication

Kineticsi

  1. KM=1.8 mM for KHCO31 Publication
  2. KM=0.46 mM for phosphoenolpyruvate1 Publication

pH dependencei

Optimum pH is 6.8.1 Publication

Temperature dependencei

Optimum temperature is 62 degrees Celsius.1 Publication

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB

GO - Biological processi

  1. carbon fixation Source: UniProtKB
  2. oxaloacetate metabolic process Source: UniProtKB
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14528.
MTHE187420:GJNM-945-MONOMER.
SABIO-RKO27026.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylase (EC:4.1.1.31)
Short name:
PEPC
Short name:
PEPCase
Gene namesi
Name:ppcA
Ordered Locus Names:MTH_943
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Phosphoenolpyruvate carboxylasePRO_0000309600Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi187420.MTH943.

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 2 family.Curated

Phylogenomic databases

eggNOGiCOG1892.
KOiK01595.
OMAiDEYMPDY.

Family and domain databases

HAMAPiMF_01904. PEPcase_type2.
InterProiIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006677. UCP006677. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

O27026-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVPRCMSTQ HPDNVNPPFF AEEPELGGED EIREAYYVFS HLGCDEQMWD
60 70 80 90 100
CEGKEVDNYV VKKLLTKYQA FFRDHVLGED LRLTLRVPNP TVERAEAKIL
110 120 130 140 150
LETLESIPRS YDTASLFYGM DAAPVFEVIL PMTSSSSCLN RIHSYYLDFV
160 170 180 190 200
KGKERLQLAD GVTVKEWIGE FRPDEINVIP LFEDHEGMLN AAKITGEYLD
210 220 230 240 250
GKDIQEQRVF LARSDPAMNY GMISATLLNR IALSDFRDLE EESGVKLYPI
260 270 280 290 300
IGMGSAPFRG NLRPDNVEDV TWEYRGAYTF TVQSSFKYDH EPSDVIRGIK
310 320 330 340 350
KLRSVKPGRA AEIERESVLE IISAYCREYR RQVMDLVDII NRVARYVPGR
360 370 380 390 400
RKRKLHIGLF GYSRSMGNVS LPRAITFTAA LYSLGVPPEL LGFNALSSGD
410 420 430 440 450
LEFIEEVYPG LGRDLHDAAR YANPESPFLS PEVKSSFEEY LEPEYDEGHM
460 470 480
KTTEEIIRAL RINRTANLQE LILEAASQRK FLG
Length:483
Mass (Da):55,078
Last modified:November 13, 2007 - v2
Checksum:iDA053C2E9C7F565A
GO

Sequence cautioni

The sequence AAB85441.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85441.1. Different initiation.
PIRiD69226.
RefSeqiNP_276080.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85441; AAB85441; MTH_943.
GeneIDi1471351.
KEGGimth:MTH943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85441.1 . Different initiation.
PIRi D69226.
RefSeqi NP_276080.1. NC_000916.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH943.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85441 ; AAB85441 ; MTH_943 .
GeneIDi 1471351.
KEGGi mth:MTH943.

Phylogenomic databases

eggNOGi COG1892.
KOi K01595.
OMAi DEYMPDY.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14528.
MTHE187420:GJNM-945-MONOMER.
SABIO-RK O27026.

Family and domain databases

HAMAPi MF_01904. PEPcase_type2.
InterProi IPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
Pfami PF14010. PEPcase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006677. UCP006677. 1 hit.
SUPFAMi SSF51621. SSF51621. 1 hit.
TIGRFAMsi TIGR02751. PEPCase_arch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "The phosphoenolpyruvate carboxylase from Methanothermobacter thermautotrophicus has a novel structure."
    Patel H.M., Kraszewski J.L., Mukhopadhyay B.
    J. Bacteriol. 186:5129-5137(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiCAPPA_METTH
AccessioniPrimary (citable) accession number: O27026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3