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O27026

- CAPPA_METTH

UniProt

O27026 - CAPPA_METTH

Protein

Phosphoenolpyruvate carboxylase

Gene

ppcA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.1 Publication

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.1 Publication

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Inhibited by NaCl, KCl, ATP, ADP, GTP and aspartate. Unlike E.coli, not regulated by acetyl-CoA.1 Publication

    Kineticsi

    1. KM=1.8 mM for KHCO31 Publication
    2. KM=0.46 mM for phosphoenolpyruvate1 Publication

    pH dependencei

    Optimum pH is 6.8.1 Publication

    Temperature dependencei

    Optimum temperature is 62 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB
    2. phosphoenolpyruvate carboxylase activity Source: UniProtKB

    GO - Biological processi

    1. carbon fixation Source: UniProtKB
    2. oxaloacetate metabolic process Source: UniProtKB
    3. tricarboxylic acid cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14528.
    MTHE187420:GJNM-945-MONOMER.
    SABIO-RKO27026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase (EC:4.1.1.31)
    Short name:
    PEPC
    Short name:
    PEPCase
    Gene namesi
    Name:ppcA
    Ordered Locus Names:MTH_943
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Phosphoenolpyruvate carboxylasePRO_0000309600Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi187420.MTH943.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG1892.
    KOiK01595.
    OMAiDEYMPDY.

    Family and domain databases

    HAMAPiMF_01904. PEPcase_type2.
    InterProiIPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF14010. PEPcase_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006677. UCP006677. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR02751. PEPCase_arch. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O27026-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVPRCMSTQ HPDNVNPPFF AEEPELGGED EIREAYYVFS HLGCDEQMWD    50
    CEGKEVDNYV VKKLLTKYQA FFRDHVLGED LRLTLRVPNP TVERAEAKIL 100
    LETLESIPRS YDTASLFYGM DAAPVFEVIL PMTSSSSCLN RIHSYYLDFV 150
    KGKERLQLAD GVTVKEWIGE FRPDEINVIP LFEDHEGMLN AAKITGEYLD 200
    GKDIQEQRVF LARSDPAMNY GMISATLLNR IALSDFRDLE EESGVKLYPI 250
    IGMGSAPFRG NLRPDNVEDV TWEYRGAYTF TVQSSFKYDH EPSDVIRGIK 300
    KLRSVKPGRA AEIERESVLE IISAYCREYR RQVMDLVDII NRVARYVPGR 350
    RKRKLHIGLF GYSRSMGNVS LPRAITFTAA LYSLGVPPEL LGFNALSSGD 400
    LEFIEEVYPG LGRDLHDAAR YANPESPFLS PEVKSSFEEY LEPEYDEGHM 450
    KTTEEIIRAL RINRTANLQE LILEAASQRK FLG 483
    Length:483
    Mass (Da):55,078
    Last modified:November 13, 2007 - v2
    Checksum:iDA053C2E9C7F565A
    GO

    Sequence cautioni

    The sequence AAB85441.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85441.1. Different initiation.
    PIRiD69226.
    RefSeqiNP_276080.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85441; AAB85441; MTH_943.
    GeneIDi1471351.
    KEGGimth:MTH943.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85441.1 . Different initiation.
    PIRi D69226.
    RefSeqi NP_276080.1. NC_000916.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH943.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85441 ; AAB85441 ; MTH_943 .
    GeneIDi 1471351.
    KEGGi mth:MTH943.

    Phylogenomic databases

    eggNOGi COG1892.
    KOi K01595.
    OMAi DEYMPDY.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14528.
    MTHE187420:GJNM-945-MONOMER.
    SABIO-RK O27026.

    Family and domain databases

    HAMAPi MF_01904. PEPcase_type2.
    InterProi IPR007566. PEP_COase_arc-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF14010. PEPcase_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006677. UCP006677. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR02751. PEPCase_arch. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. "The phosphoenolpyruvate carboxylase from Methanothermobacter thermautotrophicus has a novel structure."
      Patel H.M., Kraszewski J.L., Mukhopadhyay B.
      J. Bacteriol. 186:5129-5137(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiCAPPA_METTH
    AccessioniPrimary (citable) accession number: O27026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3