Reviewed, UniProtKB/Swiss-Prot O27002 (FMDC_METTH)

Names and origin

Protein names

Recommended name:
    Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C
    EC=1.2.99.5
Alternative name(s):
    Molybdenum-containing formylmethanofuran dehydrogenase I subunit C

Gene names

Name:	fmdC
Ordered Locus Names:	MTH_918

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize formylmethanofuran. This enzyme is oxygen-labile.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Molybdenum.
Enzyme regulation	Inactivated by cyanide.
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.
Subunit structure	Consists of five subunits; fmdA, fmdB, fmdC, fmdD, and fmdE.
Induction	By growth on molybdenum, under anaerobic conditions.
Sequence similarities	In the N-terminal section; belongs to the fwdC/fmdC family. In the C-terminal section; belongs to the molybdenum dinucleotide binding protein family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C		PRO_0000144192
Regions
	Repeat	76 – 88	13	1
	Repeat	95 – 107	13	2
	Repeat	114 – 126	13	3
	Repeat	140 – 152	13	4
	Repeat	159 – 171	13	5
	Repeat	178 – 190	13	6
	Repeat	197 – 209	13	7
	Region	76 – 209	134	7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-I-X-[IV]-X-G

Sequences

	Sequence		Length	Mass (Da)
	O27002-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DBC32FF73A5DDF05	FASTA	400	43,288
	10 20 30 40 50 60 MGFVLVPKSD FQIPLEADTI RPDLFEGLDL DEIRSLQVYE GNIKRPLGEF FEIAETPHAD 70 80 90 100 110 120 QLIRIDGDVS RVKYIGSGMK SGKIIINGDV GLQLGCEMKG GEIEVNGNVS SWIGMEMHGG 130 140 150 160 170 180 TIKINGNAGD YVGCAYRGEW RGMKGGKIII QGNAGNNIGG GMMAGEIYIG GDAGNFCGIR 190 200 210 220 230 240 MNGGEITVRG DAGRAPGAEM VSGIIKIHGR ISSLLPGFKE ISTFKEDGSL MILFKGDLSE 250 260 270 280 290 300 KNPEGNLYIN YNKNLHILEN ETDEGRVITK KGIKVIYNSG STIREGQIIK GGNKLTDDYI 310 320 330 340 350 360 DECARCCISP EDYKLLGEPE NVVVSSHGNE VVLRAVEDPG IQMGTIFIPR GIWANVLTPP 370 380 390 400 YTESTGSPMY KGVPVYLRKA SQGERILSAE ELVEEYGVGK

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85416.1.
PIR	H69222.
RefSeq	NP_276055.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O27002.
Genome annotation databases
GeneID	1471326.
GenomeReviews	Gene locus MTH_918 in contig AE000666_GR.
KEGG	mth:MTH918.
NMPDR	fig|187420.1.peg.910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27002.
OMA	LECEVIT.
Enzyme and pathway databases
BioCyc	MetaCyc:FMDCMAUTO-MON. MTHE187420:MTH918-MON.
BRENDA	1.2.99.5. 270.
Family and domain databases
InterPro	IPR012048. Formylmethanofuran_DH_csu/dsu. IPR017550. Formylmethanofuran_DH_suC. IPR002489. Glu_synthase_C. IPR006657. MPT_dinuc_bd. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF01493. GXGXG. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
PIRSF	PIRSF036633. FmdC_D. 1 hit.
TIGRFAMs	TIGR03122. one_C_dehyd_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FMDC_METTH

Accession

Primary (citable) accession number: O27002

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)