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Protein

Probable porphobilinogen deaminase

Gene

hemC

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneBy similarityNote: Binds 1 dipyrromethane group covalently.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Probable porphobilinogen deaminase (hemC)
  3. Putative uroporphyrinogen-III synthase (hemD)
  4. no protein annotated in this organism
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPorphyrin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:hemC
Ordered Locus Names:MTH_874
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001430271 – 289Probable porphobilinogen deaminaseAdd BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233S-(dipyrrolylmethanemethyl)cysteineBy similarity1

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH874.

Structurei

3D structure databases

ProteinModelPortaliO26960.
SMRiO26960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiarCOG04299. Archaea.
COG0181. LUCA.
KOiK01749.
OMAiCQVPIGV.
OrthoDBiPOG093Z0BW7.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiView protein in InterPro
IPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiView protein in Pfam
PF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiView protein in PROSITE
PS00533. PORPHOBILINOGEN_DEAM. 1 hit.

Sequencei

Sequence statusi: Complete.

O26960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAGTRGSRL ALVQTNHVIE MLSEVCKEKI EKKIIKTKGD RIRDSQLYSM
60 70 80 90 100
DSRGLFTREL DMAVLNEEVD LAVHSLKDVP SDLDPDLAIA AVPPRESPAE
110 120 130 140 150
VLVSRLDWED LPQGSKLGTS SLRREAFCNH HQKNFKMEPL RGNIDTRIRK
160 170 180 190 200
VMDGEVHATI MAEAGLKRLG LEEHIKRRFP VEYFTPAAGQ GALAVITRAD
210 220 230 240 250
SELISSIGRI THHPSLQEVT AEKTLLRELG AGCQCPLGVI GRATGNQLTL
260 270 280
YAVLLTREGE MLRKVTVRGP LAEAEDIGKK AAKEMEDYI
Length:289
Mass (Da):32,026
Last modified:January 1, 1998 - v1
Checksum:i71F894DCB5EDFEC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85372.1.
PIRiG69216.

Genome annotation databases

EnsemblBacteriaiAAB85372; AAB85372; MTH_874.
KEGGimth:MTH_874.
PATRICifig|187420.15.peg.858.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHEM3_METTH
AccessioniPrimary (citable) accession number: O26960
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families