Aspartate-semialdehyde dehydrogenase - Methanobacterium thermoautotrophicum (strain Delta H)

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O26890 (DHAS_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aspartate-semialdehyde dehydrogenase
Short name=ASA dehydrogenase
Short name=ASADH
EC=1.2.1.11

Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase

Gene names

Name:	asd
Ordered Locus Names:	MTH_799

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate By similarity. HAMAP MF_02121
Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP⁺ = L-4-aspartyl phosphate + NADPH. HAMAP MF_02121
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4. HAMAP MF_02121 Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. HAMAP MF_02121 Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5. HAMAP MF_02121
Subunit structure	Homodimer By similarity. HAMAP MF_02121
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoleucine biosynthetic process Inferred from electronic annotation. Source: InterPro methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 347

347

Aspartate-semialdehyde dehydrogenase HAMAP MF_02121

PRO_0000141398

Regions

Nucleotide binding

10 – 13

NADP By similarity

Nucleotide binding

37 – 38

NADP By similarity

Nucleotide binding

177 – 178

NADP By similarity

Nucleotide binding

327 – 328

NADP By similarity

Sites

Active site

147

Acyl-thioester intermediate By similarity

Active site

240

Proton acceptor By similarity

Binding site

108

Phosphate By similarity

Binding site

174

Substrate By similarity

Binding site

200

Substrate By similarity

Binding site

203

Phosphate By similarity

Binding site

233

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O26890 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F0CCFA7D4B3531D3
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FASTA

347

38,031

        10         20         30         40         50         60 
MVNVGVLGAT GMVGQRFIQM LDKHPEFELT TLAASSRSAG KPYGEVANWY LDCEMPESVR 

        70         80         90        100        110        120 
DMEVVETDPS AVGDVDILFS ALPADVARKV EPKFAEKYIV ASNASAMRME PDVPLVIPEV 

       130        140        150        160        170        180 
NPEFLDLIEV QQRRRGWDGF IVTNPNCSTI ALTLTLKPIY DAYTIKRVYV STMQAVSGAG 

       190        200        210        220        230        240 
YNGVPSMAIL DNLVPFIGGE EEKIETETLH LLGELDEGVV KPASFGVSAS CHRVPVVDGH 

       250        260        270        280        290        300 
TEAVFIELDD EFDIDDVREA MDKFRGLPQK LGLHSAPEKP VVVRDEENRP QPRMDRDMDG 

       310        320        330        340 
GMAVTVGRLR EDAAFKNSLR YVLVGHNTVR GAAGASILNA ELINEIL

« Hide

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.

Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85299.1.
PIR	E69206.
RefSeq	NP_275938.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26890.
SMR	O26890. Positions 2-345.
ModBase	Search...
Protein-protein interaction databases
STRING	O26890.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471207.
GenomeReviews	Gene locus MTH_799 in contig AE000666_GR.
KEGG	mth:MTH799.
NMPDR	fig\|187420.1.peg.793.
Phylogenomic databases
eggNOG	arNOG04383.
HOGENOM	HBG518238.
OMA	GAGYNGV.
PhylomeDB	O26890.
ProtClustDB	PRK08664.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH799-MONOMER.
Family and domain databases
HAMAP	MF_02121. ASADH. [Tree]
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR005676. Asp_semi-ald_DH_pep-lack. IPR012080. Asp_semialdehyde_DH. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_dimer_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00133.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00978. Asd_EA. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_METTH

Accession

Primary (citable) accession number: O26890

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents