Reviewed, UniProtKB/Swiss-Prot O26890 (DHAS_METTH)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	MTH_799

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the second step in the common metabolic pathway to synthesize Thr and Met from Asp.
Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Aspartate-semialdehyde dehydrogenase		PRO_0000141398
Sites
	Active site	147	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26890-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F0CCFA7D4B3531D3	FASTA	347	38,031
	10 20 30 40 50 60 MVNVGVLGAT GMVGQRFIQM LDKHPEFELT TLAASSRSAG KPYGEVANWY LDCEMPESVR 70 80 90 100 110 120 DMEVVETDPS AVGDVDILFS ALPADVARKV EPKFAEKYIV ASNASAMRME PDVPLVIPEV 130 140 150 160 170 180 NPEFLDLIEV QQRRRGWDGF IVTNPNCSTI ALTLTLKPIY DAYTIKRVYV STMQAVSGAG 190 200 210 220 230 240 YNGVPSMAIL DNLVPFIGGE EEKIETETLH LLGELDEGVV KPASFGVSAS CHRVPVVDGH 250 260 270 280 290 300 TEAVFIELDD EFDIDDVREA MDKFRGLPQK LGLHSAPEKP VVVRDEENRP QPRMDRDMDG 310 320 330 340 GMAVTVGRLR EDAAFKNSLR YVLVGHNTVR GAAGASILNA ELINEIL

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85299.1.
PIR	E69206.
RefSeq	NP_275938.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471207.
GenomeReviews	Gene locus MTH_799 in contig AE000666_GR.
KEGG	mth:MTH799.
NMPDR	fig|187420.1.peg.793.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O26890.
OMA	O26890. GHTECIS.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH799-MON.
BRENDA	1.2.1.11. 270.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR005676. Asp_semi-ald_DH_pep-lack. IPR012080. Asp_semialdehyde_DH. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR00978. asd_EA. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_METTH

Accession

Primary (citable) accession number: O26890

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)