O26868 (TYSY_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.-

Gene names

Name:	thyA
Ordered Locus Names:	MTH_774

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May catalyze the biosynthesis of dTMP using an unknown cosubstrate By similarity. HAMAP MF_01686
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_01686
Subunit structure	Monomer By similarity. HAMAP MF_01686
Subcellular location	Cytoplasm By similarity HAMAP MF_01686.
Sequence similarities	Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.
Sequence caution	The sequence AAB85277.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 222	221	Putative thymidylate synthase HAMAP MF_01686		PRO_0000141058
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26868 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1A01DE87215FC09B	FASTA	222	25,556
	10 20 30 40 50 60 MAYSIEVNEI ADGWKKLVEK IMHDGREIRD ERGSLTREVM NTVVTIKKPL GKSDDFYHIR 70 80 90 100 110 120 RGSLLNIKVP EGYFWSGEKL EKYSEQFLSG DRKGFVYTYG NRLRAHFGVD QLDEAIRRLK 130 140 150 160 170 180 NCTESRRATM VTWDPPEDTA SDEVPCMILV DFKIRDGRLF TTALWRSHDI YGAWFPNAVG 190 200 210 220 LAYLADHVAA EVGVEVGHIT IHSISAHIYE VNFKEAEEVI KW

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85277.1. Different initiation.
PIR	E69203.
RefSeq	NP_275916.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26868.
ModBase	Search...
Protein-protein interaction databases
STRING	O26868.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471182.
GenomeReviews	Gene locus MTH_774 in contig AE000666_GR.
KEGG	mth:MTH774.
NMPDR	fig|187420.1.peg.771.
Phylogenomic databases
eggNOG	arNOG05586.
HOGENOM	HBG540931.
OMA	GAYPANL.
ProtClustDB	PRK00956.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH774-MONOMER.
Family and domain databases
HAMAP	MF_01686. Thymidy_synth_arch. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR014620. Thymidylate_synthase_arc. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PIRSF	PIRSF036752. TSase_MJ051. 1 hit.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03283. Thy_syn_methano. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_METTH

Accession

Primary (citable) accession number: O26868

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program