O26860 (AROA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MTH_766

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088332

Sequences

	Sequence		Length	Mass (Da)
	O26860 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 395BDDB8C5262AC9	FASTA	419	44,748
	10 20 30 40 50 60 MDLTVEKSGN LEGTVKAPPS KSYTHRAVII AALAEGVSEI RDPLIAEDTL SSLNACRAFG 70 80 90 100 110 120 IRVDEGDAWT VHGSGGELET PDDVIYLGNS GTTLRLMTSV AGLAENYTVL TGDESLRTRP 130 140 150 160 170 180 MQPLLDALRP LGVEALSSRM NGLPPIIVRG GLRGGSTSIR GDVSSQFISS ILIAAPLTEG 190 200 210 220 230 240 VEVMVEGDFI SRPYVDMTVD VMERFSVPVD YSEGTFRVEP AVYRGLDYTV EGDYSSASYL 250 260 270 280 290 300 AGAVAAAGGD VLIENLFRDS RQGDRIILDI ISDMGAEVRR GEDHVRIAST GELSGVSVNL 310 320 330 340 350 360 HDAPDLLPTV AVLGALATGR TEIGGVEHAR YKETDRISTC AAELRRLGVD VTELPDGMII 370 380 390 400 410 EGGASGGTVW SHGDHRLAMA FTLIGLREGI TIRDAEVFSV SFPDFPERMM QIGCRMNLS

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85269.1.
PIR	D69202.
RefSeq	NP_275908.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26860.
ModBase	Search...
Protein-protein interaction databases
STRING	O26860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471174.
GenomeReviews	Gene locus MTH_766 in contig AE000666_GR.
KEGG	mth:MTH766.
NMPDR	fig|187420.1.peg.763.
Phylogenomic databases
eggNOG	arNOG05032.
HOGENOM	HBG646626.
OMA	FADHRIA.
PhylomeDB	O26860.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH766-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_METTH

Accession

Primary (citable) accession number: O26860

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program