Reviewed, UniProtKB/Swiss-Prot O26839 (HEM2_METTH)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	MTH_744

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Zinc By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	porphobilinogen synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Delta-aminolevulinic acid dehydratase		PRO_0000140525
Regions
	Region	123 – 141	19	Zinc-binding By similarity
Sites
	Active site	253	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26839-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 29EDC9060F39A170	FASTA	326	35,841
	10 20 30 40 50 60 MSDNMEFPTK RMRRLRKSPQ IRNILRETRL HPSDLIYPMF VSEKLGRGDV EAIDTMPGQF 70 80 90 100 110 120 RYSVDDAVSE ASRLEDEGLS SVLIFGMPSA KDELASAAHD PHGVVQRTVR RLKEETDLVV 130 140 150 160 170 180 MTDVCLCQYT SHGHCGIVVD GEIVNDETLE VLSRIALSHA EAGADVVAPS DMMDGRVAAI 190 200 210 220 230 240 RRSLDDAGFQ DTLIMSYAVK YASAFYAPFR GAVSSAPAFG DRRSYQMDPA NIDEALIEAE 250 260 270 280 290 300 LDLREGADIL MVKPALAYLD VIGKVRERFS VPLAAYNVSG EYSMLKAAIK SGYLTDEAIY 310 320 ESILSIKRAG ADLIISHFAP DLLGVI

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85248.1.
PIR	E69199.
RefSeq	NP_275887.1.
3D structure databases
HSSP	HSSP built from PDB template 1E51 based on UniProtKB P13716.
ModBase	Search...
Protein-protein interaction databases
STRING	O26839.
Genome annotation databases
GeneID	1471152.
GenomeReviews	Gene locus MTH_744 in contig AE000666_GR.
KEGG	mth:MTH744.
NMPDR	fig|187420.1.peg.742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O26839.
OMA	KFILFGV.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH744-MON.
BRENDA	4.2.1.24. 270.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_METTH

Accession

Primary (citable) accession number: O26839

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)