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O26839

- HEM2_METTH

UniProt

O26839 - HEM2_METTH

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251Zinc; catalyticBy similarity
Metal bindingi127 – 1271Zinc; catalyticBy similarity
Metal bindingi135 – 1351Zinc; catalyticBy similarity
Active sitei200 – 2001Schiff-base intermediate with substrateBy similarity
Binding sitei210 – 2101Substrate 1By similarity
Binding sitei222 – 2221Substrate 1By similarity
Metal bindingi238 – 2381MagnesiumBy similarity
Active sitei253 – 2531Schiff-base intermediate with substrateBy similarity
Binding sitei279 – 2791Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-746-MONOMER.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:MTH_744
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Delta-aminolevulinic acid dehydratasePRO_0000140525Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi187420.MTH744.

Structurei

3D structure databases

ProteinModelPortaliO26839.
SMRiO26839. Positions 10-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
KOiK01698.
OMAiDMILTYF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26839-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDNMEFPTK RMRRLRKSPQ IRNILRETRL HPSDLIYPMF VSEKLGRGDV
60 70 80 90 100
EAIDTMPGQF RYSVDDAVSE ASRLEDEGLS SVLIFGMPSA KDELASAAHD
110 120 130 140 150
PHGVVQRTVR RLKEETDLVV MTDVCLCQYT SHGHCGIVVD GEIVNDETLE
160 170 180 190 200
VLSRIALSHA EAGADVVAPS DMMDGRVAAI RRSLDDAGFQ DTLIMSYAVK
210 220 230 240 250
YASAFYAPFR GAVSSAPAFG DRRSYQMDPA NIDEALIEAE LDLREGADIL
260 270 280 290 300
MVKPALAYLD VIGKVRERFS VPLAAYNVSG EYSMLKAAIK SGYLTDEAIY
310 320
ESILSIKRAG ADLIISHFAP DLLGVI
Length:326
Mass (Da):35,841
Last modified:January 1, 1998 - v1
Checksum:i29EDC9060F39A170
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85248.1.
PIRiE69199.
RefSeqiNP_275887.1. NC_000916.1.
WP_010876383.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85248; AAB85248; MTH_744.
GeneIDi1471152.
KEGGimth:MTH744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85248.1 .
PIRi E69199.
RefSeqi NP_275887.1. NC_000916.1.
WP_010876383.1. NC_000916.1.

3D structure databases

ProteinModelPortali O26839.
SMRi O26839. Positions 10-324.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85248 ; AAB85248 ; MTH_744 .
GeneIDi 1471152.
KEGGi mth:MTH744.

Phylogenomic databases

eggNOGi COG0113.
KOi K01698.
OMAi DMILTYF.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
BioCyci MTHE187420:GJNM-746-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiHEM2_METTH
AccessioniPrimary (citable) accession number: O26839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3