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O26839

- HEM2_METTH

UniProt

O26839 - HEM2_METTH

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi125 – 1251Zinc; catalyticBy similarity
    Metal bindingi127 – 1271Zinc; catalyticBy similarity
    Metal bindingi135 – 1351Zinc; catalyticBy similarity
    Active sitei200 – 2001Schiff-base intermediate with substrateBy similarity
    Binding sitei210 – 2101Substrate 1By similarity
    Binding sitei222 – 2221Substrate 1By similarity
    Metal bindingi238 – 2381MagnesiumBy similarity
    Active sitei253 – 2531Schiff-base intermediate with substrateBy similarity
    Binding sitei279 – 2791Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-746-MONOMER.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:MTH_744
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Delta-aminolevulinic acid dehydratasePRO_0000140525Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi187420.MTH744.

    Structurei

    3D structure databases

    ProteinModelPortaliO26839.
    SMRiO26839. Positions 10-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    KOiK01698.
    OMAiDMILTYF.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O26839-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNMEFPTK RMRRLRKSPQ IRNILRETRL HPSDLIYPMF VSEKLGRGDV    50
    EAIDTMPGQF RYSVDDAVSE ASRLEDEGLS SVLIFGMPSA KDELASAAHD 100
    PHGVVQRTVR RLKEETDLVV MTDVCLCQYT SHGHCGIVVD GEIVNDETLE 150
    VLSRIALSHA EAGADVVAPS DMMDGRVAAI RRSLDDAGFQ DTLIMSYAVK 200
    YASAFYAPFR GAVSSAPAFG DRRSYQMDPA NIDEALIEAE LDLREGADIL 250
    MVKPALAYLD VIGKVRERFS VPLAAYNVSG EYSMLKAAIK SGYLTDEAIY 300
    ESILSIKRAG ADLIISHFAP DLLGVI 326
    Length:326
    Mass (Da):35,841
    Last modified:January 1, 1998 - v1
    Checksum:i29EDC9060F39A170
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85248.1.
    PIRiE69199.
    RefSeqiNP_275887.1. NC_000916.1.
    WP_010876383.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85248; AAB85248; MTH_744.
    GeneIDi1471152.
    KEGGimth:MTH744.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85248.1 .
    PIRi E69199.
    RefSeqi NP_275887.1. NC_000916.1.
    WP_010876383.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O26839.
    SMRi O26839. Positions 10-324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85248 ; AAB85248 ; MTH_744 .
    GeneIDi 1471152.
    KEGGi mth:MTH744.

    Phylogenomic databases

    eggNOGi COG0113.
    KOi K01698.
    OMAi DMILTYF.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    BioCyci MTHE187420:GJNM-746-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiHEM2_METTH
    AccessioniPrimary (citable) accession number: O26839
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3