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Protein

tRNA (guanine(10)-N2)-dimethyltransferase

Gene

trmG10

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N2) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m2G) or N(2)-dimethylguanosine (m22G) (By similarity).By similarity

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(10) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(10) in tRNA.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(10)-N2)-dimethyltransferase (EC:2.1.1.213)
Alternative name(s):
tRNA:G10 dimethyltransferase
Gene namesi
Name:trmG10
Ordered Locus Names:MTH_724
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404821 – 336tRNA (guanine(10)-N2)-dimethyltransferaseAdd BLAST336

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi187420.MTH724.

Structurei

3D structure databases

ProteinModelPortaliO26820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 147THUMPPROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Contains 1 THUMP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00047. Archaea.
COG1041. LUCA.
KOiK07446.
OMAiDIDEQMV.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP_dom.
IPR005885. TrmG10.
[Graphical view]
PfamiPF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01177. TIGR01177. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMMVLLSQE HPELPSAELR SVLMSEGIDF SVIESGSGYE VIDAPRSTWK
60 70 80 90 100
ILKKRLAYAH EISEVIGYAA ADDLDDAAEE IDWSKYVRES FAVRIRKLCG
110 120 130 140 150
DVDSRTLERT IGAIIKEDTG LKVDLEKPWT LIRPVLINDR FILTRRLAVI
160 170 180 190 200
SKEHFNQARP HKRPFFYPGS MSPKLARCMV NLSGVKAGDR ILDPFCGTGG
210 220 230 240 250
ILIEAGLMGV RVVGADIDWR MVEGTRENLQ HYGITDFEVI RSDARDLRLD
260 270 280 290 300
EKVDAIVTDP PYGISASTAG EKSEKLYREF LDSAHSNLAE GGMICMAAPH
310 320 330
YLDLESLIDE RFSIRERYSM RMHRSLTRVI RVIERV
Length:336
Mass (Da):38,020
Last modified:January 1, 1998 - v1
Checksum:iD305B3FFADE1F5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85229.1.
PIRiG69196.
RefSeqiWP_010876363.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85229; AAB85229; MTH_724.
GeneIDi1470685.
KEGGimth:MTH_724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85229.1.
PIRiG69196.
RefSeqiWP_010876363.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO26820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85229; AAB85229; MTH_724.
GeneIDi1470685.
KEGGimth:MTH_724.

Phylogenomic databases

eggNOGiarCOG00047. Archaea.
COG1041. LUCA.
KOiK07446.
OMAiDIDEQMV.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP_dom.
IPR005885. TrmG10.
[Graphical view]
PfamiPF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01177. TIGR01177. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMG10_METTH
AccessioniPrimary (citable) accession number: O26820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.