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O26819 (CIMA_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate By similarity. HAMAP-Rule MF_01028

Catalytic activity

Acetyl-CoA + pyruvate + H2O = CoA + (2R)-2-hydroxy-2-methylbutanedioate. HAMAP-Rule MF_01028

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3. HAMAP-Rule MF_01028

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01028

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Putative (R)-citramalate synthase CimA HAMAP-Rule MF_01028
PRO_0000140453

Sequences

Sequence LengthMass (Da)Tools
O26819 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: FCB4D5CDC2F5A115

FASTA49653,705
        10         20         30         40         50         60 
MQVRVLDTTL RDGEQTPGVS LTPEEKLRIA LKIDALGADI IEAGSAITSE GEREGIRKIT 

        70         80         90        100        110        120 
SEGLRAEICS FARAVREDID AAISCDVDSV HLVVPTSDLH LEHKLRKTRE EVLEQAVDCT 

       130        140        150        160        170        180 
EYAVDHGILV ELSAEDSTRS DMDFLRTIFR EGIEAGAERI CACDTVGILT PERSYEFYRG 

       190        200        210        220        230        240 
LSELGAPLSV HCHNDFGLAV ANSLAGLRAG ASEVHATING IGERAGNAAL EEVVVALKSL 

       250        260        270        280        290        300 
YDVDTSINIE MLYETSRMVA RMTGVYLQPN KAIVGENAFA HESGIHADGV LKKAETYEPI 

       310        320        330        340        350        360 
TPEMVGHGRG FVMGKHIGTH ALRKRLDELG MKVADDKLME IFRRVKTLGD MGKCVTDVDL 

       370        380        390        400        410        420 
QAIAEDVLGV MEDKVVDLQE VTIVSGNRVT PTASVKLRVD DREVLEAGTG VGPVDAAIVA 

       430        440        450        460        470        480 
IKKSLEDFAD ITLEEYHVDA ITGGTDALID VVIKLRHGDR IISARSTQPD IIMASVEAFL 

       490 
SGVNRLLANE KSEGTH 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85228.1.
PIRF69196.
RefSeqNP_275866.1. NC_000916.1.

3D structure databases

ProteinModelPortalO26819.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH723.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85228; AAB85228; MTH_723.
GeneID1470684.
KEGGmth:MTH723.

Phylogenomic databases

eggNOGCOG0119.
KOK09011.
OMAERICACD.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-725-MONOMER.
UniPathwayUPA00047; UER00066.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01028. CimA.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR024890. Citramalate_synthase_CimA.
IPR011830. LEU1_arch.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. SSF110921. 1 hit.
TIGRFAMsTIGR02090. LEU1_arch. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCIMA_METTH
AccessionPrimary (citable) accession number: O26819
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways