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O26806 (GUAAB_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing] subunit B

EC=6.3.5.2
Alternative name(s):
GMP synthetase
Gene names
Name:guaAB
Ordered Locus Names:MTH_710
OrganismMethanobacterium thermoautotrophicum (strain Delta H)
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345

Catalytic activity

ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345

Subunit structure

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.

Sequence similarities

Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Sequence caution

The sequence AAB85215.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGMP biosynthesis
Purine biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processGMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GMP synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345
PRO_0000140245

Regions

Domain2 – 183182GMPS ATP-PPase
Nucleotide binding29 – 357ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O26806 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: F2DCF6ED202CAEC1

FASTA30834,403
        10         20         30         40         50         60 
MLNPSDFIEE AVEEIRSTVG NEKAIIALSG GVDSSVASVL AGRAIGDNLT AVFVDHGLLR 

        70         80         90        100        110        120 
EGEAEYVRKT FSERLNFRYI DASEEFLKEL EGVTDPEEKR KIIGRVFIEV FERVAEEIGA 

       130        140        150        160        170        180 
RYLVQGTIAP DWIESEGQIK SHHNVALPHG LVLEIVEPIR ELYKDEVREI GLELGLPREM 

       190        200        210        220        230        240 
IQRQPFPGPG LAVRIVGEIT REKIEICRRA NAIVEEEVVE SGLHESLWQY FAVLTDTMVT 

       250        260        270        280        290        300 
GVKGDVRDFG YLVVIRMVES LDAMTASVPE LPWDLIKRIS KRITAEIPEV THVALSVSDK 


PPSTIEFA 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Delta H.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85215.1. Different initiation.
PIRH69194.
RefSeqNP_275853.1. NC_000916.1.

3D structure databases

ProteinModelPortalO26806.
SMRO26806. Positions 2-308.
ModBaseSearch...

Protein-protein interaction databases

STRINGO26806.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1470671.
GenomeReviewsGene locus MTH_710 in contig AE000666_GR.
KEGGmth:MTH710.
NMPDRfig|187420.1.peg.708.

Phylogenomic databases

eggNOGarNOG04539.
HOGENOMHBG391775.
OMAQGTIAPD.
PhylomeDBO26806.
ProtClustDBPRK00919.

Enzyme and pathway databases

BioCycMTHE187420:MTH710-MONOMER.

Family and domain databases

HAMAPMF_00345. GMP_synthase_B.
[Tree]
InterProIPR001674. GMP_synth_C.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01951.
PfamPF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00884. GuaA_Cterm. 1 hit.
PROSITEPS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUAAB_METTH
AccessionPrimary (citable) accession number: O26806
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: December 14, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families