O26806 (GUAAB_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	MTH_710

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.
Sequence caution	The sequence AAB85215.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140245
Regions
	Domain	2 – 183	182	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26806 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: F2DCF6ED202CAEC1	FASTA	308	34,403
	10 20 30 40 50 60 MLNPSDFIEE AVEEIRSTVG NEKAIIALSG GVDSSVASVL AGRAIGDNLT AVFVDHGLLR 70 80 90 100 110 120 EGEAEYVRKT FSERLNFRYI DASEEFLKEL EGVTDPEEKR KIIGRVFIEV FERVAEEIGA 130 140 150 160 170 180 RYLVQGTIAP DWIESEGQIK SHHNVALPHG LVLEIVEPIR ELYKDEVREI GLELGLPREM 190 200 210 220 230 240 IQRQPFPGPG LAVRIVGEIT REKIEICRRA NAIVEEEVVE SGLHESLWQY FAVLTDTMVT 250 260 270 280 290 300 GVKGDVRDFG YLVVIRMVES LDAMTASVPE LPWDLIKRIS KRITAEIPEV THVALSVSDK PPSTIEFA

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85215.1. Different initiation.
PIR	H69194.
RefSeq	NP_275853.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26806.
SMR	O26806. Positions 2-308.
ModBase	Search...
Protein-protein interaction databases
STRING	O26806.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470671.
GenomeReviews	Gene locus MTH_710 in contig AE000666_GR.
KEGG	mth:MTH710.
NMPDR	fig|187420.1.peg.708.
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	QGTIAPD.
PhylomeDB	O26806.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH710-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_METTH

Accession

Primary (citable) accession number: O26806

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	December 14, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program