O26805 (GUAAA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	MTH_709

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140225
Regions
	Domain	3 – 186	184	Glutamine amidotransferase type-1
Sites
	Active site	77	1	Nucleophile By similarity
	Active site	164	1	By similarity
	Active site	166	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26805 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B8A288792A7D1F05	FASTA	186	21,130
	10 20 30 40 50 60 MFMILIINNH GQYNHRIHRT LRYLQIPSEL VPNTTPLDEI ISRDPEGLIL GGGPSLERSG 70 80 90 100 110 120 NCVEYIQKLE IPILGICLGH QLIAKAYGGE VATAEAESYA QIELEILDED DIFRGLGPRM 130 140 150 160 170 180 SVWASHKDEV KKLPDEFDVL ATSSICEVEA MKHHDKPVYG IQFHPEVHHT REGHRVFENF YDVCRS

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85214.1.
PIR	F69194.
RefSeq	NP_275852.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26805.
ModBase	Search...
Protein-protein interaction databases
STRING	O26805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470670.
GenomeReviews	Gene locus MTH_709 in contig AE000666_GR.
KEGG	mth:MTH709.
NMPDR	fig|187420.1.peg.707.
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
PhylomeDB	O26805.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH709-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METTH

Accession

Primary (citable) accession number: O26805

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program