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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit E

Gene

gatE

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14999.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit E (EC:6.3.5.-)
Short name:
Glu-ADT subunit E
Gene namesi
Name:gatE
Ordered Locus Names:MTH_707
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400751 – 619Glutamyl-tRNA(Gln) amidotransferase subunit EAdd BLAST619

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.

Protein-protein interaction databases

STRINGi187420.MTH707.

Structurei

Secondary structure

1619
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Beta strandi9 – 17Combined sources9
Beta strandi24 – 26Combined sources3
Beta strandi28 – 31Combined sources4
Beta strandi38 – 44Combined sources7
Turni60 – 62Combined sources3
Helixi64 – 67Combined sources4
Beta strandi68 – 73Combined sources6
Turni75 – 77Combined sources3
Turni80 – 84Combined sources5
Helixi93 – 105Combined sources13
Beta strandi112 – 114Combined sources3
Beta strandi117 – 120Combined sources4
Beta strandi124 – 144Combined sources21
Beta strandi147 – 158Combined sources12
Beta strandi162 – 167Combined sources6
Beta strandi170 – 174Combined sources5
Beta strandi181 – 186Combined sources6
Helixi194 – 208Combined sources15
Beta strandi221 – 227Combined sources7
Beta strandi234 – 237Combined sources4
Helixi242 – 244Combined sources3
Helixi245 – 268Combined sources24
Beta strandi273 – 278Combined sources6
Helixi282 – 284Combined sources3
Helixi290 – 293Combined sources4
Beta strandi295 – 303Combined sources9
Turni307 – 309Combined sources3
Beta strandi310 – 315Combined sources6
Helixi320 – 329Combined sources10
Turni339 – 343Combined sources5
Helixi349 – 358Combined sources10
Beta strandi363 – 372Combined sources10
Helixi374 – 393Combined sources20
Beta strandi398 – 402Combined sources5
Beta strandi404 – 406Combined sources3
Beta strandi408 – 413Combined sources6
Beta strandi420 – 422Combined sources3
Helixi433 – 440Combined sources8
Helixi447 – 456Combined sources10
Helixi462 – 470Combined sources9
Turni474 – 476Combined sources3
Helixi488 – 492Combined sources5
Turni493 – 496Combined sources4
Helixi497 – 501Combined sources5
Turni511 – 513Combined sources3
Helixi514 – 520Combined sources7
Beta strandi523 – 525Combined sources3
Helixi528 – 530Combined sources3
Helixi531 – 537Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D6FX-ray3.15C/D1-619[»]
ProteinModelPortaliO26803.
SMRiO26803.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO26803.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatE subfamily.Curated

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
KOiK03330.
OMAiTSGFQRT.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWEKVGLKM GLEIHQQLDT ESKLFCPCRT ELTDSEPDHD IVRNLRPTQS
60 70 80 90 100
ELGKFDRAAF EEAMRKLHFH YENYHEETCL VEADEEPPHP LNPEALEIAV
110 120 130 140 150
TIALLLNMRV VDEFHTMRKQ VIDGSNTGGF QRTGLVATDG HLETPQGTVK
160 170 180 190 200
IENLCLEEDA ARRIRETGDG VVFRLDRLGI PLVEITTDPS MSDPQQLREV
210 220 230 240 250
AYQIGQILRS TRVKRGLGTI RQDLNISIRD GARVEVKGVQ DLDLIPEIVE
260 270 280 290 300
REVKRQLSLV EIRDTLQERG AVVEDKIFDV SEVFADTESR IISSAESVLA
310 320 330 340 350
VKLRGFDGLI GVEIQPGRRL GTEMADYAKK RGVSGIFHTD ELPAYGITEE
360 370 380 390 400
EVRGLRDAVG ASQGDAVVMV AHERVTAENA LREVIRRAEM AIQGVPEETR
410 420 430 440 450
KALPDGNTQY LRPLPTSSRM YLETDIPLFR IEDDLLEGIR RNLPELPSEK
460 470 480 490 500
KERIMRDYGL SEDLASQLVK RNLVDEFEAL TEFRVDTTVI ASLLAYTLRE
510 520 530 540 550
LRREGHDVDG LGLDELRDAI KLLEVGKISK DALRDIVACM ADEGLAAEDA
560 570 580 590 600
ARKLNLLLLA EDEIESIIQE IVEGNLDMIS ERGMGAMGPL MGQAMGRLRG
610
RADGKVVNRI LREKIQERL
Length:619
Mass (Da):69,533
Last modified:January 1, 1998 - v1
Checksum:iD362944674300A32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85212.1.
PIRiD69194.
RefSeqiWP_010876346.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85212; AAB85212; MTH_707.
GeneIDi1470668.
KEGGimth:MTH_707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85212.1.
PIRiD69194.
RefSeqiWP_010876346.1. NC_000916.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D6FX-ray3.15C/D1-619[»]
ProteinModelPortaliO26803.
SMRiO26803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85212; AAB85212; MTH_707.
GeneIDi1470668.
KEGGimth:MTH_707.

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
KOiK03330.
OMAiTSGFQRT.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14999.

Miscellaneous databases

EvolutionaryTraceiO26803.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATE_METTH
AccessioniPrimary (citable) accession number: O26803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.