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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit E

Gene

gatE

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14999.
MTHE187420:GJNM-709-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit E (EC:6.3.5.-)
Short name:
Glu-ADT subunit E
Gene namesi
Name:gatE
Ordered Locus Names:MTH_707
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 619619Glutamyl-tRNA(Gln) amidotransferase subunit EPRO_0000140075Add
BLAST

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.

Protein-protein interaction databases

STRINGi187420.MTH707.

Structurei

Secondary structure

1
619
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 64Combined sources
Beta strandi9 – 179Combined sources
Beta strandi24 – 263Combined sources
Beta strandi28 – 314Combined sources
Beta strandi38 – 447Combined sources
Turni60 – 623Combined sources
Helixi64 – 674Combined sources
Beta strandi68 – 736Combined sources
Turni75 – 773Combined sources
Turni80 – 845Combined sources
Helixi93 – 10513Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi117 – 1204Combined sources
Beta strandi124 – 14421Combined sources
Beta strandi147 – 15812Combined sources
Beta strandi162 – 1676Combined sources
Beta strandi170 – 1745Combined sources
Beta strandi181 – 1866Combined sources
Helixi194 – 20815Combined sources
Beta strandi221 – 2277Combined sources
Beta strandi234 – 2374Combined sources
Helixi242 – 2443Combined sources
Helixi245 – 26824Combined sources
Beta strandi273 – 2786Combined sources
Helixi282 – 2843Combined sources
Helixi290 – 2934Combined sources
Beta strandi295 – 3039Combined sources
Turni307 – 3093Combined sources
Beta strandi310 – 3156Combined sources
Helixi320 – 32910Combined sources
Turni339 – 3435Combined sources
Helixi349 – 35810Combined sources
Beta strandi363 – 37210Combined sources
Helixi374 – 39320Combined sources
Beta strandi398 – 4025Combined sources
Beta strandi404 – 4063Combined sources
Beta strandi408 – 4136Combined sources
Beta strandi420 – 4223Combined sources
Helixi433 – 4408Combined sources
Helixi447 – 45610Combined sources
Helixi462 – 4709Combined sources
Turni474 – 4763Combined sources
Helixi488 – 4925Combined sources
Turni493 – 4964Combined sources
Helixi497 – 5015Combined sources
Turni511 – 5133Combined sources
Helixi514 – 5207Combined sources
Beta strandi523 – 5253Combined sources
Helixi528 – 5303Combined sources
Helixi531 – 5377Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D6FX-ray3.15C/D1-619[»]
ProteinModelPortaliO26803.
SMRiO26803. Positions 1-538.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO26803.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatE subfamily.Curated

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
KOiK03330.
OMAiTSGFQRT.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWEKVGLKM GLEIHQQLDT ESKLFCPCRT ELTDSEPDHD IVRNLRPTQS
60 70 80 90 100
ELGKFDRAAF EEAMRKLHFH YENYHEETCL VEADEEPPHP LNPEALEIAV
110 120 130 140 150
TIALLLNMRV VDEFHTMRKQ VIDGSNTGGF QRTGLVATDG HLETPQGTVK
160 170 180 190 200
IENLCLEEDA ARRIRETGDG VVFRLDRLGI PLVEITTDPS MSDPQQLREV
210 220 230 240 250
AYQIGQILRS TRVKRGLGTI RQDLNISIRD GARVEVKGVQ DLDLIPEIVE
260 270 280 290 300
REVKRQLSLV EIRDTLQERG AVVEDKIFDV SEVFADTESR IISSAESVLA
310 320 330 340 350
VKLRGFDGLI GVEIQPGRRL GTEMADYAKK RGVSGIFHTD ELPAYGITEE
360 370 380 390 400
EVRGLRDAVG ASQGDAVVMV AHERVTAENA LREVIRRAEM AIQGVPEETR
410 420 430 440 450
KALPDGNTQY LRPLPTSSRM YLETDIPLFR IEDDLLEGIR RNLPELPSEK
460 470 480 490 500
KERIMRDYGL SEDLASQLVK RNLVDEFEAL TEFRVDTTVI ASLLAYTLRE
510 520 530 540 550
LRREGHDVDG LGLDELRDAI KLLEVGKISK DALRDIVACM ADEGLAAEDA
560 570 580 590 600
ARKLNLLLLA EDEIESIIQE IVEGNLDMIS ERGMGAMGPL MGQAMGRLRG
610
RADGKVVNRI LREKIQERL
Length:619
Mass (Da):69,533
Last modified:January 1, 1998 - v1
Checksum:iD362944674300A32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85212.1.
PIRiD69194.
RefSeqiWP_010876346.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85212; AAB85212; MTH_707.
GeneIDi1470668.
KEGGimth:MTH_707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85212.1.
PIRiD69194.
RefSeqiWP_010876346.1. NC_000916.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D6FX-ray3.15C/D1-619[»]
ProteinModelPortaliO26803.
SMRiO26803. Positions 1-538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85212; AAB85212; MTH_707.
GeneIDi1470668.
KEGGimth:MTH_707.

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
KOiK03330.
OMAiTSGFQRT.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14999.
MTHE187420:GJNM-709-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO26803.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATE_METTH
AccessioniPrimary (citable) accession number: O26803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.