Reviewed, UniProtKB/Swiss-Prot O26802 (GATD_METTH)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA(Gln) amidotransferase subunit D
      Short name=Glu-ADT subunit D
    EC=6.3.5.-

Gene names

Name:	gatD
Ordered Locus Names:	MTH_706

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The gatDE system is specific for glutamate and does not act on aspartate. HAMAP MF_00586
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00586
Subunit structure	Heterodimer of gatD and gatE. HAMAP MF_00586
Sequence similarities	Belongs to the asparaginase 1 family. GatD subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparaginase activity Inferred from electronic annotation. Source: InterPro glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 435

435

Glutamyl-tRNA(Gln) amidotransferase subunit D HAMAP MF_00586

PRO_0000140056

Sites

Active site

101

1

By similarity

Active site

177

1

By similarity

Active site

178

1

By similarity

Active site

254

1

By similarity

Secondary structure

1

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435

Helix Strand Turn

Details...

Helix

5 – 12

8

Turn

13 – 15

3

Beta strand

21 – 25

5

Beta strand

30 – 35

6

Beta strand

44 – 50

7

Beta strand

56 – 60

5

Beta strand

68 – 70

3

Beta strand

88 – 90

3

Beta strand

92 – 99

8

Turn

108 – 110

3

Helix

121 – 125

5

Helix

127 – 131

5

Beta strand

134 – 136

3

Helix

145 – 147

3

Helix

150 – 165

16

Beta strand

169 – 174

6

Turn

177 – 179

3

Helix

180 – 190

11

Beta strand

197 – 200

4

Helix

213 – 225

13

Beta strand

230 – 242

13

Beta strand

244 – 248

5

Helix

249 – 251

3

Beta strand

252 – 254

3

Beta strand

256 – 258

3

Beta strand

263 – 268

6

Beta strand

271 – 275

5

Beta strand

304 – 308

5

Helix

315 – 323

9

Beta strand

327 – 334

8

Turn

335 – 337

3

Helix

341 – 343

3

Helix

344 – 352

9

Beta strand

357 – 361

5

Helix

374 – 381

8

Helix

392 – 402

11

Turn

403 – 405

3

Helix

409 – 417

9

Beta strand

420 – 422

3

Helix

430 – 432

3

Sequences

	Sequence		Length	Mass (Da)
	O26802-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 34D8E4205486126F	FASTA	435	47,997
	10 20 30 40 50 60 MSYQGRARKF LESASIDVGD MVLVEKPDVT YEGMVLDRAD DADDRHIVLK LENGYNIGVE 70 80 90 100 110 120 ISDARIELLE KGSEPRIELP PVEAAEDPEL PDVSIISTGG TVASIIDYRT GAVHPAFTAD 130 140 150 160 170 180 DLLRANPELL DIANIRGRAV FNILSENMKP EYWVETARAV YGEIKDGADG VVVAHGTDTM 190 200 210 220 230 240 HYTSAALSFM LRTPVPVVFT GAQRSSDRPS SDASLNIQCS VRAATSEIAE VTVCMHATMD 250 260 270 280 290 300 DLSCHLHRGV KVRKMHTSRR DTFRSMNALP LAEVTPDGIK ILEENYRKRG SDELELSDRV 310 320 330 340 350 360 EERVAFIKSY PGISPDIIKW HLDEGYRGIV IEGTGLGHCP DTLIPVIGEA HDMGVPVAMT 370 380 390 400 410 420 SQCLNGRVNM NVYSTGRRLL QAGVIPCDDM LPEVAYVKMC WVLGQTDDPE MAREMMRENI 430 AGEINERTSI AYFRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.
[2]	"Domain-specific recruitment of amide amino acids for protein synthesis." Tumbula D.L., Becker H.D., Chang W.-Z., Soell D. Nature 407:106-110(2000) [PubMed: 10993083] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000666 Genomic DNA. Translation: AAB85211.1.

PIR

C69194.

RefSeq

NP_275849.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D6F	X-ray	3.15	A/B	1-435	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

O26802.

Genome annotation databases

GeneID

1470667.

GenomeReviews

Gene locus MTH_706 in contig AE000666_GR.

KEGG

mth:MTH706.

NMPDR

fig|187420.1.peg.704.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O26802.

OMA

GVIPCED.

Enzyme and pathway databases

BioCyc

MTHE187420:MTH706-MON.

Family and domain databases

HAMAP

MF_00586.
[Tree]

InterPro

IPR006033. AsnASEI.
IPR006034. Asp/Glutamnse.
IPR011878. GatD_arch.
[Graphical view]

PANTHER

PTHR11707. Asp/Glutamnse. 1 hit.

Pfam

PF00710. Asparaginase. 1 hit.
[Graphical view]

PRINTS

PR00139. ASNGLNASE.

ProDom

PD003221. Asp/Glutamnse. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00519. asnASE_I. 1 hit.
TIGR02153. gatD_arch. 1 hit.

PROSITE

PS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GATD_METTH

Accession

Primary (citable) accession number: O26802

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)