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O26742

- PUR1_METTH

UniProt

O26742 - PUR1_METTH

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei11 – 111For GATase activityBy similarity
    Metal bindingi250 – 2501Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi297 – 2971MagnesiumBy similarity
    Metal bindingi359 – 3591MagnesiumBy similarity
    Metal bindingi360 – 3601MagnesiumBy similarity
    Metal bindingi396 – 3961Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi447 – 4471Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi450 – 4501Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-646-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:MTH_646
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1010By similarityPRO_0000029275
    Chaini11 – 474464AmidophosphoribosyltransferasePRO_0000029276Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi187420.MTH646.

    Structurei

    3D structure databases

    ProteinModelPortaliO26742.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 234224Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    KOiK00764.
    OMAiSGIPYEM.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O26742-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGESEVRDK CGIVGIYSQD KKTGVASQIY YALYALQHRG QESAGISTFN    50
    GNDILTHRGM GLVCDVFNPE KLEELKGNVG IGHVRYSTTG ESRIENSQPF 100
    WSEFQGGKIA IAHNGDIINS MELREELEEE GHNFVSTTDS EVICHLLSRE 150
    YDEKPNMIYS IKRVSEQLVG SYSLVVLLNQ DLYVVRDPVG IKPLAFARKG 200
    STQIVASETV AFDVIGAEHV RDVQPGEILH LNRGKSYWVA NAPNTRRAHC 250
    MFEYVYFARP DSVIDGRNVY RVRLNIGEAL YREHPANADV VVPVPDSSIP 300
    AAIGYSRASG IPYGEGLIKN RYVGRTFIMP TQEERETAVK LKMNPIRSEL 350
    EGKRIVLIDD SIVRGTTSRA LIDIIRDAGA EEIHLRIGCP PIKSPCYYGI 400
    AMATKKELIA STRNVEEIRR IIGVDSLGYL SIESLVECIG IKKGFLCTGC 450
    LDGDYPTPLP SDISEYEAMR SCSR 474
    Length:474
    Mass (Da):52,660
    Last modified:January 1, 1998 - v1
    Checksum:i16BAF93BBF15A0D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85151.1.
    PIRiH69185.
    RefSeqiNP_275788.1. NC_000916.1.
    WP_010876284.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85151; AAB85151; MTH_646.
    GeneIDi1470607.
    KEGGimth:MTH646.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85151.1 .
    PIRi H69185.
    RefSeqi NP_275788.1. NC_000916.1.
    WP_010876284.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O26742.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH646.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85151 ; AAB85151 ; MTH_646 .
    GeneIDi 1470607.
    KEGGi mth:MTH646.

    Phylogenomic databases

    eggNOGi COG0034.
    KOi K00764.
    OMAi SGIPYEM.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci MTHE187420:GJNM-646-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiPUR1_METTH
    AccessioniPrimary (citable) accession number: O26742
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3