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O26742

- PUR1_METTH

UniProt

O26742 - PUR1_METTH

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Protein
Amidophosphoribosyltransferase
Gene
purF, MTH_646
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111For GATase activity By similarity
Metal bindingi250 – 2501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi297 – 2971Magnesium By similarity
Metal bindingi359 – 3591Magnesium By similarity
Metal bindingi360 – 3601Magnesium By similarity
Metal bindingi396 – 3961Iron-sulfur (4Fe-4S) By similarity
Metal bindingi447 – 4471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi450 – 4501Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-646-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:MTH_646
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1010 By similarity
PRO_0000029275
Chaini11 – 474464Amidophosphoribosyltransferase
PRO_0000029276Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH646.

Structurei

3D structure databases

ProteinModelPortaliO26742.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 234224Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
KOiK00764.
OMAiSGIPYEM.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O26742-1 [UniParc]FASTAAdd to Basket

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MLGESEVRDK CGIVGIYSQD KKTGVASQIY YALYALQHRG QESAGISTFN    50
GNDILTHRGM GLVCDVFNPE KLEELKGNVG IGHVRYSTTG ESRIENSQPF 100
WSEFQGGKIA IAHNGDIINS MELREELEEE GHNFVSTTDS EVICHLLSRE 150
YDEKPNMIYS IKRVSEQLVG SYSLVVLLNQ DLYVVRDPVG IKPLAFARKG 200
STQIVASETV AFDVIGAEHV RDVQPGEILH LNRGKSYWVA NAPNTRRAHC 250
MFEYVYFARP DSVIDGRNVY RVRLNIGEAL YREHPANADV VVPVPDSSIP 300
AAIGYSRASG IPYGEGLIKN RYVGRTFIMP TQEERETAVK LKMNPIRSEL 350
EGKRIVLIDD SIVRGTTSRA LIDIIRDAGA EEIHLRIGCP PIKSPCYYGI 400
AMATKKELIA STRNVEEIRR IIGVDSLGYL SIESLVECIG IKKGFLCTGC 450
LDGDYPTPLP SDISEYEAMR SCSR 474
Length:474
Mass (Da):52,660
Last modified:January 1, 1998 - v1
Checksum:i16BAF93BBF15A0D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85151.1.
PIRiH69185.
RefSeqiNP_275788.1. NC_000916.1.
WP_010876284.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85151; AAB85151; MTH_646.
GeneIDi1470607.
KEGGimth:MTH646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85151.1 .
PIRi H69185.
RefSeqi NP_275788.1. NC_000916.1.
WP_010876284.1. NC_000916.1.

3D structure databases

ProteinModelPortali O26742.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH646.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85151 ; AAB85151 ; MTH_646 .
GeneIDi 1470607.
KEGGi mth:MTH646.

Phylogenomic databases

eggNOGi COG0034.
KOi K00764.
OMAi SGIPYEM.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci MTHE187420:GJNM-646-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPUR1_METTH
AccessioniPrimary (citable) accession number: O26742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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