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O26742

- PUR1_METTH

UniProt

O26742 - PUR1_METTH

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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111NucleophileUniRule annotation
Metal bindingi250 – 2501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi297 – 2971MagnesiumUniRule annotation
Metal bindingi359 – 3591MagnesiumUniRule annotation
Metal bindingi360 – 3601MagnesiumUniRule annotation
Metal bindingi396 – 3961Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi447 – 4471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi450 – 4501Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-646-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:MTH_646
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1010By similarityPRO_0000029275
Chaini11 – 474464AmidophosphoribosyltransferasePRO_0000029276Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH646.

Structurei

3D structure databases

ProteinModelPortaliO26742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 234224Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
KOiK00764.
OMAiSGIPYEM.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O26742-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGESEVRDK CGIVGIYSQD KKTGVASQIY YALYALQHRG QESAGISTFN
60 70 80 90 100
GNDILTHRGM GLVCDVFNPE KLEELKGNVG IGHVRYSTTG ESRIENSQPF
110 120 130 140 150
WSEFQGGKIA IAHNGDIINS MELREELEEE GHNFVSTTDS EVICHLLSRE
160 170 180 190 200
YDEKPNMIYS IKRVSEQLVG SYSLVVLLNQ DLYVVRDPVG IKPLAFARKG
210 220 230 240 250
STQIVASETV AFDVIGAEHV RDVQPGEILH LNRGKSYWVA NAPNTRRAHC
260 270 280 290 300
MFEYVYFARP DSVIDGRNVY RVRLNIGEAL YREHPANADV VVPVPDSSIP
310 320 330 340 350
AAIGYSRASG IPYGEGLIKN RYVGRTFIMP TQEERETAVK LKMNPIRSEL
360 370 380 390 400
EGKRIVLIDD SIVRGTTSRA LIDIIRDAGA EEIHLRIGCP PIKSPCYYGI
410 420 430 440 450
AMATKKELIA STRNVEEIRR IIGVDSLGYL SIESLVECIG IKKGFLCTGC
460 470
LDGDYPTPLP SDISEYEAMR SCSR
Length:474
Mass (Da):52,660
Last modified:January 1, 1998 - v1
Checksum:i16BAF93BBF15A0D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85151.1.
PIRiH69185.
RefSeqiNP_275788.1. NC_000916.1.
WP_010876284.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85151; AAB85151; MTH_646.
GeneIDi1470607.
KEGGimth:MTH646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85151.1 .
PIRi H69185.
RefSeqi NP_275788.1. NC_000916.1.
WP_010876284.1. NC_000916.1.

3D structure databases

ProteinModelPortali O26742.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH646.

Protein family/group databases

MEROPSi C44.A02.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85151 ; AAB85151 ; MTH_646 .
GeneIDi 1470607.
KEGGi mth:MTH646.

Phylogenomic databases

eggNOGi COG0034.
KOi K00764.
OMAi SGIPYEM.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci MTHE187420:GJNM-646-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPUR1_METTH
AccessioniPrimary (citable) accession number: O26742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3