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O26742 (PUR1_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:MTH_646
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter ›

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1010 By similarity
PRO_0000029275
Chain11 – 474464Amidophosphoribosyltransferase
PRO_0000029276

Regions

Domain11 – 234224Glutamine amidotransferase type-2

Sites

Active site111For GATase activity By similarity
Metal binding2501Iron-sulfur (4Fe-4S) By similarity
Metal binding2971Magnesium By similarity
Metal binding3591Magnesium By similarity
Metal binding3601Magnesium By similarity
Metal binding3961Iron-sulfur (4Fe-4S) By similarity
Metal binding4471Iron-sulfur (4Fe-4S) By similarity
Metal binding4501Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O26742 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 16BAF93BBF15A0D2

FASTA47452,660
        10         20         30         40         50         60 
MLGESEVRDK CGIVGIYSQD KKTGVASQIY YALYALQHRG QESAGISTFN GNDILTHRGM 

        70         80         90        100        110        120 
GLVCDVFNPE KLEELKGNVG IGHVRYSTTG ESRIENSQPF WSEFQGGKIA IAHNGDIINS 

       130        140        150        160        170        180 
MELREELEEE GHNFVSTTDS EVICHLLSRE YDEKPNMIYS IKRVSEQLVG SYSLVVLLNQ 

       190        200        210        220        230        240 
DLYVVRDPVG IKPLAFARKG STQIVASETV AFDVIGAEHV RDVQPGEILH LNRGKSYWVA 

       250        260        270        280        290        300 
NAPNTRRAHC MFEYVYFARP DSVIDGRNVY RVRLNIGEAL YREHPANADV VVPVPDSSIP 

       310        320        330        340        350        360 
AAIGYSRASG IPYGEGLIKN RYVGRTFIMP TQEERETAVK LKMNPIRSEL EGKRIVLIDD 

       370        380        390        400        410        420 
SIVRGTTSRA LIDIIRDAGA EEIHLRIGCP PIKSPCYYGI AMATKKELIA STRNVEEIRR 

       430        440        450        460        470 
IIGVDSLGYL SIESLVECIG IKKGFLCTGC LDGDYPTPLP SDISEYEAMR SCSR

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000666 Genomic DNA. Translation: AAB85151.1.
PIRH69185.
RefSeqNP_275788.1. NC_000916.1.

3D structure databases

ProteinModelPortalO26742.
ModBaseSearch...

Protein-protein interaction databases

STRING187420.MTH646.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85151; AAB85151; MTH_646.
GeneID1470607.
KEGGmth:MTH646.

Phylogenomic databases

eggNOGCOG0034.
KOK00764.
OMAGIPFELG.
ProtClustDBCLSK797956.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-646-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_METTH
AccessionPrimary (citable) accession number: O26742
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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