Reviewed, UniProtKB/Swiss-Prot O26662 (HMDH_METTH)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	MTH_562

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114462
Sites
	Active site	96	1	Charge relay system By similarity
	Active site	301	1	Charge relay system By similarity
	Active site	391	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26662-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C4AA45A82EF211E4	FASTA	397	42,160
	10 20 30 40 50 60 MSIMDDLMEG RIKLYEIERH VPVDEAVRIR REFIERTCGV KLEHVSNYSI DMERASRRNI 70 80 90 100 110 120 ENPIGVVQIP LGVAGPLRVR GEHADGEYYV PLATSEGALV ASVNRGCSVI TRAGGATVRV 130 140 150 160 170 180 TGDSMTRAPV IRTGSVVEAL QLREWIYENM DALREEAEST TRHGKLVKID PIIVAGSYVY 190 200 210 220 230 240 PRFVYTTGDS MGMNMVTIAT ERALELLTRE TGAHVIALSG NLCTDKKPAA VNLIEGRGKS 250 260 270 280 290 300 ITAEITVPGE MVESVLKTTP EAVVEVNTAK NLIGSAAAGS MGFNAHYANI IGAIFLATGQ 310 320 330 340 350 360 DEAHIVEGSL GVTIAEERKG DLYFAVNLPD VPLATVGGGT GLETASECLD IMGVRGGGRV 370 380 390 HAFAEIVGGA VLAGELSLMG ALAAGHLARA HSELGRG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85068.1.
PIR	D69174.
RefSeq	NP_275705.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Genome annotation databases
GeneID	1470523.
GenomeReviews	Gene locus MTH_562 in contig AE000666_GR.
KEGG	mth:MTH562.
NMPDR	fig|187420.1.peg.560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O26662.
OMA	O26662. CVAESAI.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH562-MON.
BRENDA	1.1.1.34. 270.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 2 hits.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. False negative. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_METTH

Accession

Primary (citable) accession number: O26662

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)