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Protein

CTP synthase

Gene

pyrG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei382 – 3821NucleophileUniRule annotation
Active sitei507 – 5071UniRule annotation
Active sitei509 – 5091UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-419-MONOMER.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
CTP synthetaseUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:MTH_419
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533CTP synthasePRO_0000138263Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH419.

Structurei

3D structure databases

ProteinModelPortaliO26519.
SMRiO26519. Positions 5-532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini303 – 533231Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 257257Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
KOiK01937.
OMAiFDHNITT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHLAKYIVV TGGVVSSIGK GITAASIGRI LRSYGLSVTA IKIDPYLNWD
60 70 80 90 100
SGTLNPYQHG EVFVTDDGME TDLDLGHYER FLDSDLPGEA NITTGKVYMS
110 120 130 140 150
VINKERSGDY LGSCVQIIPH ITDEIKSMIR KIADKSGAEV VLVEVGGTVG
160 170 180 190 200
DIEGQPFLEA LRQLRNEEGH ENVMFVHVTY VPYLRAAGEF KTKPTQHSTK
210 220 230 240 250
ELRSTGINPD MIICRSEMPI DSSLKRKIAH FCDVEEEAVV NAPDASSIYE
260 270 280 290 300
VPLVLDSERV GDYIVRRIEL DVDGEADLSE WAGIVESLMI DEPVVTVGIV
310 320 330 340 350
GKYVELEDSY ISIREALKHA AAHLRVRVDI EWISADDAVN EEDLSRLDSI
360 370 380 390 400
LIPGGFGERG IAGKLEAVRF ALENRVPIFG ICLGMQCMVI EFARLNGMEG
410 420 430 440 450
ANSTEFDPET PYPVIDMMEE QKRIKNMGGT MRLGSYQCRI REGTLAHEAY
460 470 480 490 500
GTELVGERHR HRFELNNEFR EELESKGLII SGTSPDDFLV EMVEIKDHPW
510 520 530
FLGCQFHPEF RSRPNRAHPL FVSFLRAALE RSR
Length:533
Mass (Da):59,564
Last modified:January 1, 1998 - v1
Checksum:iC8633AD8556E8ED9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84925.1.
PIRiE69154.
RefSeqiWP_010876058.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB84925; AAB84925; MTH_419.
GeneIDi1470380.
KEGGimth:MTH419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84925.1.
PIRiE69154.
RefSeqiWP_010876058.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO26519.
SMRiO26519. Positions 5-532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH419.

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB84925; AAB84925; MTH_419.
GeneIDi1470380.
KEGGimth:MTH419.

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
KOiK01937.
OMAiFDHNITT.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciMTHE187420:GJNM-419-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPYRG_METTH
AccessioniPrimary (citable) accession number: O26519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.