Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CTP synthase

Gene

pyrG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161Allosteric inhibitor CTP; alternateUniRule annotation
Binding sitei16 – 161UTP; alternateUniRule annotation
Binding sitei57 – 571L-glutamineUniRule annotation
Metal bindingi74 – 741MagnesiumUniRule annotation
Binding sitei74 – 741ATPUniRule annotation
Metal bindingi144 – 1441MagnesiumUniRule annotation
Binding sitei227 – 2271Allosteric inhibitor CTP; alternateUniRule annotation
Binding sitei227 – 2271UTP; alternateUniRule annotation
Binding sitei355 – 3551L-glutamine; via carbonyl oxygenUniRule annotation
Active sitei382 – 3821Nucleophile; for glutamine hydrolysisUniRule annotation
Binding sitei405 – 4051L-glutamineUniRule annotation
Binding sitei462 – 4621L-glutamine; via amide nitrogenUniRule annotation
Active sitei507 – 5071UniRule annotation
Active sitei509 – 5091UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 226ATPUniRule annotation
Nucleotide bindingi151 – 1533Allosteric inhibitor CTPUniRule annotation
Nucleotide bindingi191 – 1966Allosteric inhibitor CTP; alternateUniRule annotation
Nucleotide bindingi191 – 1966UTP; alternateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-419-MONOMER.
UniPathwayiUPA00159; UER00277.

Protein family/group databases

MEROPSiC26.964.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
Cytidine 5'-triphosphate synthaseUniRule annotation
Cytidine triphosphate synthetaseUniRule annotation
Short name:
CTP synthetaseUniRule annotation
Short name:
CTPSUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:MTH_419
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533CTP synthasePRO_0000138263Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH419.

Structurei

3D structure databases

ProteinModelPortaliO26519.
SMRiO26519. Positions 5-532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini303 – 533231Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 270270Amidoligase domainUniRule annotationAdd
BLAST
Regioni383 – 3864L-glutamine bindingUniRule annotation

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
KOiK01937.
OMAiFDHNITT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHLAKYIVV TGGVVSSIGK GITAASIGRI LRSYGLSVTA IKIDPYLNWD
60 70 80 90 100
SGTLNPYQHG EVFVTDDGME TDLDLGHYER FLDSDLPGEA NITTGKVYMS
110 120 130 140 150
VINKERSGDY LGSCVQIIPH ITDEIKSMIR KIADKSGAEV VLVEVGGTVG
160 170 180 190 200
DIEGQPFLEA LRQLRNEEGH ENVMFVHVTY VPYLRAAGEF KTKPTQHSTK
210 220 230 240 250
ELRSTGINPD MIICRSEMPI DSSLKRKIAH FCDVEEEAVV NAPDASSIYE
260 270 280 290 300
VPLVLDSERV GDYIVRRIEL DVDGEADLSE WAGIVESLMI DEPVVTVGIV
310 320 330 340 350
GKYVELEDSY ISIREALKHA AAHLRVRVDI EWISADDAVN EEDLSRLDSI
360 370 380 390 400
LIPGGFGERG IAGKLEAVRF ALENRVPIFG ICLGMQCMVI EFARLNGMEG
410 420 430 440 450
ANSTEFDPET PYPVIDMMEE QKRIKNMGGT MRLGSYQCRI REGTLAHEAY
460 470 480 490 500
GTELVGERHR HRFELNNEFR EELESKGLII SGTSPDDFLV EMVEIKDHPW
510 520 530
FLGCQFHPEF RSRPNRAHPL FVSFLRAALE RSR
Length:533
Mass (Da):59,564
Last modified:January 1, 1998 - v1
Checksum:iC8633AD8556E8ED9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84925.1.
PIRiE69154.
RefSeqiWP_010876058.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB84925; AAB84925; MTH_419.
GeneIDi1470380.
KEGGimth:MTH_419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84925.1.
PIRiE69154.
RefSeqiWP_010876058.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO26519.
SMRiO26519. Positions 5-532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH419.

Protein family/group databases

MEROPSiC26.964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB84925; AAB84925; MTH_419.
GeneIDi1470380.
KEGGimth:MTH_419.

Phylogenomic databases

eggNOGiarCOG00063. Archaea.
COG0504. LUCA.
KOiK01937.
OMAiFDHNITT.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciMTHE187420:GJNM-419-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPYRG_METTH
AccessioniPrimary (citable) accession number: O26519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.