O26330 (GSA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	MTH_228

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP MF_00375
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP MF_00375
Subcellular location	Cytoplasm Potential HAMAP MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP MF_00375		PRO_0000120486
Amino acid modifications
	Modified residue	259	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26330 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2BF17CDC8074C4B2	FASTA	420	45,727
	10 20 30 40 50 60 MKSEELFRRA SNVLPGGVSS PVRRFDPHPF FAAGGSGCLL ESVDGESYID YCLAYGPLIL 70 80 90 100 110 120 GHAHPRVVEA VNEQIKRGTT YGVPTEGEIE LAEAIIERVP CAEMVRFTNS GTEATMAAVR 130 140 150 160 170 180 LARAFTGRDR IVKFEGSYHG AHDYVLVRPG SGAATAPDSP GIPVDTVRNT LTVPFNHEEA 190 200 210 220 230 240 MAELIEGAGE DIACILVEPV MGNIGCIEPE NGYLQFLRDI TRENDIILIF DEVITGFRLA 250 260 270 280 290 300 PGGAQEYYRV EPDLVTLGKI VGGGFPMGAL AGRREIMENI SPAGNVYQAG TFNGNPVSVT 310 320 330 340 350 360 AGRETLRLLD GRMYSDLERK GSTLRAGLRD LLSDLDLEYQ VTGPASMFQL YFTGEEVRNY 370 380 390 400 410 420 GDAKKSDTVL FMEYFHGLLE RGVFIPPSQF ECCFISAAHE SEHIEATLEA AEEVLSGLKN

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB84734.1.
PIR	C69128.
RefSeq	NP_275371.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26330.
ModBase	Search...
Protein-protein interaction databases
STRING	O26330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470189.
GenomeReviews	Gene locus MTH_228 in contig AE000666_GR.
KEGG	mth:MTH228.
NMPDR	fig|187420.1.peg.226.
Phylogenomic databases
eggNOG	arNOG04972.
HOGENOM	HBG725944.
OMA	RVPCAEM.
PhylomeDB	O26330.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH228-MONOMER.
Family and domain databases
HAMAP	MF_00375. HemL_aminotrans_3. [Tree]
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K01845.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. HemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_METTH

Accession

Primary (citable) accession number: O26330

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program