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O26290 (MDH_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113489

Regions

Nucleotide binding7 – 137NAD By similarity
Nucleotide binding120 – 1223NAD By similarity

Sites

Active site1771Proton acceptor By similarity
Binding site841Substrate By similarity
Binding site901Substrate By similarity
Binding site971NAD By similarity
Binding site1221Substrate By similarity
Binding site1531Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O26290 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: AA9C2E5B8827F9C9

FASTA32535,933
        10         20         30         40         50         60 
MKVSIIGSTG RVGRATALCL AEEEAVKTLH LISRKESLEQ NLGEVLDMSD ALAAKGVSVK 

        70         80         90        100        110        120 
LENSADIENV YGSRIVVITA GVPRTADMDR DDLAFKNGRI VADYARQIAR FAPDSIILVV 

       130        140        150        160        170        180 
TNPVDVMTYV ALRYSGFHPS RVFGLGNHLD SLRLKNYMAR HFNVHVSEVH TRVIGQHGPY 

       190        200        210        220        230        240 
MVPLISSTSI GGIPIEHYAR RDYFSGYKKF DLKKTIDKVI HAGSNIISRK GATEYGPAFA 

       250        260        270        280        290        300 
ISNIVTTILN DERRILTVST LMEGEIDGIR DVCLGVPVKL GKNGIEGVVP VLMDRDEREA 

       310        320 
FREAANHVRD STRRVMEFLD EELPL 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB84694.1.
PIRE69118.
RefSeqNP_275331.1. NC_000916.1.

3D structure databases

ProteinModelPortalO26290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB84694; AAB84694; MTH_188.
GeneID1470149.
KEGGmth:MTH188.

Phylogenomic databases

eggNOGCOG0039.
KOK00024.
OMAYGQNDIC.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14545.
MTHE187420:GJNM-188-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_METTH
AccessionPrimary (citable) accession number: O26290
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families