O26285 (ARGB_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	MTH_183

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_A
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_A.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Probable acetylglutamate kinase HAMAP MF_00082_A		PRO_0000112698
Regions
	Region	60 – 61	2	Substrate binding By similarity
Sites
	Binding site	82	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Site	25	1	Transition state stabilizer By similarity
	Site	251	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26285 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 79BE60FED8CB8B77	FASTA	293	31,700
	10 20 30 40 50 60 MKTVNILVEA LPYIKKFHRK KIMIKYGGHA MIDEAAMDST ARDTVLLKYV GMEPVVVHGG 70 80 90 100 110 120 GPEISRAMNK MGKEPKFIEG LRVTDEETME IVKMVLVGKI NTSIVSKICY HGGRAIGLSG 130 140 150 160 170 180 KDSNLLLARK RAPHVVRDDE TGERREIDLG LVGEIESVDP GIINMLTDNN YIPVISPIGV 190 200 210 220 230 240 DRDANTLNLN ADTVAGEVAA GIGAEKLIVL TDVPGILEDP SDPDTLIRRI SVDELSDLVK 250 260 270 280 290 SGIVEGGMLP KTLTCIQAIN DGVSSAHIID GRVEHSLLLE IFTKKGIGTM ITE

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB84689.1.
PIR	F69111.
RefSeq	NP_275326.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26285.
SMR	O26285. Positions 3-292.
ModBase	Search...
Protein-protein interaction databases
STRING	O26285.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470144.
GenomeReviews	Gene locus MTH_183 in contig AE000666_GR.
KEGG	mth:MTH183.
NMPDR	fig|187420.1.peg.181.
Phylogenomic databases
eggNOG	arNOG04941.
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	O26285.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH183-MONOMER.
Family and domain databases
HAMAP	MF_00082_A. ArgB_A. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_METTH

Accession

Primary (citable) accession number: O26285

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program