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Protein

Nicotinamide-nucleotide adenylyltransferase

Gene

MTH_150

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + nicotinamide ribonucleotide = diphosphate + NAD+.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinamide-nucleotide adenylyltransferase (MTH_150)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-150-MONOMER.
BRENDAi2.7.7.1. 3256.
SABIO-RKO26253.
UniPathwayiUPA00253; UER00600.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide-nucleotide adenylyltransferase (EC:2.7.7.1)
Alternative name(s):
NAD(+) diphosphorylase
NAD(+) pyrophosphorylase
NMN adenylyltransferase
Gene namesi
Ordered Locus Names:MTH_150
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Nicotinamide-nucleotide adenylyltransferasePRO_0000134995Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi187420.MTH150.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi14 – 2310Combined sources
Turni24 – 263Combined sources
Beta strandi28 – 347Combined sources
Beta strandi41 – 455Combined sources
Helixi49 – 6214Combined sources
Helixi67 – 693Combined sources
Beta strandi70 – 745Combined sources
Helixi81 – 9111Combined sources
Beta strandi96 – 994Combined sources
Helixi103 – 1119Combined sources
Beta strandi115 – 1173Combined sources
Turni124 – 1263Combined sources
Helixi129 – 13810Combined sources
Helixi143 – 1453Combined sources
Helixi148 – 1569Combined sources
Helixi159 – 1668Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EJ2X-ray1.90A1-178[»]
1HYBX-ray2.00A1-178[»]
1M8FX-ray2.40A1-178[»]
1M8GX-ray2.00A1-178[»]
1M8JX-ray2.40A1-178[»]
1M8KX-ray3.00A/B/C1-178[»]
4YP5X-ray2.21A/B/C1-178[»]
4YP6X-ray1.90A/B/C1-178[»]
4YP7X-ray2.30A/B/C1-178[»]
ProteinModelPortaliO26253.
SMRiO26253. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO26253.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00972. Archaea.
COG1056. LUCA.
KOiK00952.
OMAiNSVWVSH.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00243. NMN_adenylyltr.
InterProiIPR004821. Cyt_trans-like.
IPR006418. NMN_Atrans_arc.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01527. arch_NMN_Atrans. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

O26253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLLVGRMQ PFHRGHLQVI KSILEEVDEL IICIGSAQLS HSIRDPFTAG
60 70 80 90 100
ERVMMLTKAL SENGIPASRY YIIPVQDIEC NALWVGHIKM LTPPFDRVYS
110 120 130 140 150
GNPLVQRLFS EDGYEVTAPP LFYRDRYSGT EVRRRMLDDG DWRSLLPESV
160 170
VEVIDEINGV ERIKHLAKKE VSELGGIS
Length:178
Mass (Da):20,207
Last modified:December 1, 2000 - v2
Checksum:iA1B4F41BA3FF98AE
GO

Sequence cautioni

The sequence AAB84656.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84656.1. Different initiation.
PIRiA69067.
RefSeqiWP_048060754.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB84656; AAB84656; MTH_150.
GeneIDi1470111.
KEGGimth:MTH_150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84656.1. Different initiation.
PIRiA69067.
RefSeqiWP_048060754.1. NC_000916.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EJ2X-ray1.90A1-178[»]
1HYBX-ray2.00A1-178[»]
1M8FX-ray2.40A1-178[»]
1M8GX-ray2.00A1-178[»]
1M8JX-ray2.40A1-178[»]
1M8KX-ray3.00A/B/C1-178[»]
4YP5X-ray2.21A/B/C1-178[»]
4YP6X-ray1.90A/B/C1-178[»]
4YP7X-ray2.30A/B/C1-178[»]
ProteinModelPortaliO26253.
SMRiO26253. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB84656; AAB84656; MTH_150.
GeneIDi1470111.
KEGGimth:MTH_150.

Phylogenomic databases

eggNOGiarCOG00972. Archaea.
COG1056. LUCA.
KOiK00952.
OMAiNSVWVSH.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00600.
BioCyciMTHE187420:GJNM-150-MONOMER.
BRENDAi2.7.7.1. 3256.
SABIO-RKO26253.

Miscellaneous databases

EvolutionaryTraceiO26253.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00243. NMN_adenylyltr.
InterProiIPR004821. Cyt_trans-like.
IPR006418. NMN_Atrans_arc.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01527. arch_NMN_Atrans. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

Entry informationi

Entry nameiNADM_METTH
AccessioniPrimary (citable) accession number: O26253
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.