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Protein

Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)

Gene

cbiT

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.1 Publication

Catalytic activityi

Cobalt-precorrin-6B + S-adenosyl-L-methionine = cobalt-precorrin-7 + S-adenosyl-L-homocysteine + CO2.UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 8 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route).UniRule annotation
Proteins known to be involved in the 10 steps of the subpathway in this organism are:
  1. Sirohydrochlorin cobaltochelatase (cbiX)
  2. no protein annotated in this organism
  3. Probable cobalt-factor III C(17)-methyltransferase (cbiH)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. Cobalt-precorrin-5B C(1)-methyltransferase (cbiD)
  7. Cobalt-precorrin-6A reductase (cbiJ)
  8. Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating) (cbiT)
  9. Cobalt-precorrin-8 methylmutase (cbiC)
  10. Cobyrinate a,c-diamide synthase (cbiA)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171S-adenosyl-L-methionineUniRule annotation1 Publication
Binding sitei62 – 621S-adenosyl-L-methionineUniRule annotation1 Publication
Binding sitei91 – 911S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-146-MONOMER.
BRENDAi2.1.1.289. 3256.
UniPathwayiUPA00148; UER00229.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)UniRule annotation (EC:2.1.1.196UniRule annotation)
Gene namesi
Name:cbiTUniRule annotation
Ordered Locus Names:MTH_146
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)PRO_0000134941Add
BLAST

Proteomic databases

PRIDEiO26249.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi187420.MTH146.

Structurei

Secondary structure

1
192
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Helixi19 – 2911Combined sources
Beta strandi36 – 416Combined sources
Helixi46 – 527Combined sources
Beta strandi55 – 639Combined sources
Helixi65 – 7713Combined sources
Beta strandi84 – 896Combined sources
Helixi91 – 955Combined sources
Beta strandi101 – 1077Combined sources
Helixi113 – 12210Combined sources
Beta strandi124 – 13411Combined sources
Helixi137 – 14913Combined sources
Beta strandi155 – 16713Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi179 – 1835Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F38X-ray2.40A/B/C/D1-192[»]
1KXZX-ray2.70A/B/C/D/E/F/G/H1-192[»]
1L3BX-ray2.65A/B/C/D/E/F/G/H1-192[»]
1L3CX-ray2.31A/B/C/D1-192[»]
1L3IX-ray1.95A/B/C/D/E/F1-192[»]
ProteinModelPortaliO26249.
SMRiO26249. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO26249.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 455S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00977. Archaea.
COG2242. LUCA.
KOiK02191.
OMAiKFVYAID.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00786. CbiT.
InterProiIPR023475. CbiT.
IPR014008. Cbl_synth_MTase_CbiT.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02469. CbiT. 1 hit.

Sequencei

Sequence statusi: Complete.

O26249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPDDEFIKN PSVPGPTAME VRCLIMCLAE PGKNDVAVDV GCGTGGVTLE
60 70 80 90 100
LAGRVRRVYA IDRNPEAIST TEMNLQRHGL GDNVTLMEGD APEALCKIPD
110 120 130 140 150
IDIAVVGGSG GELQEILRII KDKLKPGGRI IVTAILLETK FEAMECLRDL
160 170 180 190
GFDVNITELN IARGRALDRG TMMVSRNPVA LIYTGVSHEN KD
Length:192
Mass (Da):20,714
Last modified:January 1, 1998 - v1
Checksum:i7BFA35D8BD3C3982
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84652.1.
PIRiD69061.
RefSeqiWP_010875785.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB84652; AAB84652; MTH_146.
GeneIDi1470107.
KEGGimth:MTH_146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB84652.1.
PIRiD69061.
RefSeqiWP_010875785.1. NC_000916.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F38X-ray2.40A/B/C/D1-192[»]
1KXZX-ray2.70A/B/C/D/E/F/G/H1-192[»]
1L3BX-ray2.65A/B/C/D/E/F/G/H1-192[»]
1L3CX-ray2.31A/B/C/D1-192[»]
1L3IX-ray1.95A/B/C/D/E/F1-192[»]
ProteinModelPortaliO26249.
SMRiO26249. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH146.

Proteomic databases

PRIDEiO26249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB84652; AAB84652; MTH_146.
GeneIDi1470107.
KEGGimth:MTH_146.

Phylogenomic databases

eggNOGiarCOG00977. Archaea.
COG2242. LUCA.
KOiK02191.
OMAiKFVYAID.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00229.
BioCyciMTHE187420:GJNM-146-MONOMER.
BRENDAi2.1.1.289. 3256.

Miscellaneous databases

EvolutionaryTraceiO26249.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00786. CbiT.
InterProiIPR023475. CbiT.
IPR014008. Cbl_synth_MTase_CbiT.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02469. CbiT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase."
    Keller J.P., Smith P.M., Benach J., Christendat D., deTitta G.T., Hunt J.F.
    Structure 10:1475-1487(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiCBIT_METTH
AccessioniPrimary (citable) accession number: O26249
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a precorrin-8w decarboxylase.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.