Reviewed, UniProtKB/Swiss-Prot O26156 (IDSA_METTH)

Names and origin

Protein names

Recommended name:
    Bifunctional short chain isoprenyl diphosphate synthase
Including the following 2 domains:
    1- Recommended name:
            Farnesyl pyrophosphate synthetase
                Short name=FPP synthetase
              EC=2.5.1.1
        Alternative name(s):
            Dimethylallyltranstransferase
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10

Gene names

Name:	idsA
Ordered Locus Names:	MTH_50

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.
Cofactor	Magnesium.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Bifunctional short chain isoprenyl diphosphate synthase		PRO_0000123971

Sequences

	Sequence		Length	Mass (Da)
	O26156-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: BCF3A285A207916E	FASTA	325	35,485
	10 20 30 40 50 60 MMEVMDILRK YSEMADERIR ESISDITPET LLRASEHLIT AGGKKIRPSL ALLSSEAVGG 70 80 90 100 110 120 DPGDAAGVAA AIELIHTFSL IHDDIMDDDE IRRGEPAVHV LWGEPMAILA GDVLFSKAFE 130 140 150 160 170 180 AVIRNGDSEM VKEALAVVVD SCVKICEGQA LDMGFEERLD VTEEEYMEMI YKKTAALIAA 190 200 210 220 230 240 ATKAGAIMGG GSPQEIAALE DYGRCIGLAF QIHDDYLDVV SDEESLGKPV GSDIAEGKMT 250 260 270 280 290 300 LMVVKALERA SEKDRERLIS ILGSGDEKLV AEAIEIFERY GATEYAHAVA LDHVRMAKER 310 320 LEVLEESDAR EALAMIADFV LEREH

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB84557.1.
PIR	G69165.
RefSeq	NP_275193.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1470012.
GenomeReviews	Gene locus MTH_50 in contig AE000666_GR.
KEGG	mth:MTH50.
NMPDR	fig|187420.1.peg.48.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O26156.
OMA	O26156. SKACVEV.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH50-MON.
BRENDA	2.5.1.1. 270. 2.5.1.10. 270.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDSA_METTH

Accession

Primary (citable) accession number: O26156

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)