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Protein

Enolase

Gene

eno

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147SubstrateUniRule annotation1
Binding sitei157SubstrateUniRule annotation1
Active sitei200Proton donorUniRule annotation1
Metal bindingi236MagnesiumUniRule annotation1
Metal bindingi281MagnesiumUniRule annotation1
Binding sitei281SubstrateUniRule annotation1
Metal bindingi308MagnesiumUniRule annotation1
Binding sitei308SubstrateUniRule annotation1
Active sitei333Proton acceptorUniRule annotation1
Binding sitei333Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei384SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14533
MTHE187420:G1G16-43-MONOMER
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:MTH_43
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340281 – 416EnolaseAdd BLAST416

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH43

Structurei

3D structure databases

ProteinModelPortaliO26149
SMRiO26149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 363Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169 Archaea
COG0148 LUCA
KOiK01689
OMAiQEFLVVP
OrthoDBiPOG093Z03D9

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

O26149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESIIEDVRV RKILDSRGNP TVEVDVITWN GFGRAAAPSG ASTGSREVAA
60 70 80 90 100
FPSGGVDEII TEVEDIISSE LIGMDAVDLQ DIDLVLKEID GTENLSSLGG
110 120 130 140 150
NTVVAVSMAT AKAAASSYNM PLYRFLGGNL ATSIPYPLGN MINGGAHAGK
160 170 180 190 200
NAPDIQEFLV VPVGAEDITE AVFANAAVHK RIRELIQKKD PSFAGGKGDE
210 220 230 240 250
GGWVPSLSNG DALEIQATAC EEVTDELGVE VRPSLDLAAS EFWDPEIEKY
260 270 280 290 300
VYRQENVQKD TGEQIEFVKE IIETYDMYYV EDPLHEGDLE GFAELTSLVG
310 320 330 340 350
DRCMICGDDI FVTNREILRE GIEMGAANAI IIKPNQIGTL TDTYLTVKLA
360 370 380 390 400
LENRYTPVVS HRSGETTDDT IAHLAVAFGA PLIKTGAIGG ERIAKLNELI
410
RIQEEIPYSR MADLPF
Length:416
Mass (Da):44,941
Last modified:January 1, 1998 - v1
Checksum:i9B2298E46E98B570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB84550.1
PIRiA69156
RefSeqiWP_010875683.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB84550; AAB84550; MTH_43
GeneIDi1470005
KEGGimth:MTH_43
PATRICifig|187420.15.peg.41

Similar proteinsi

Entry informationi

Entry nameiENO_METTH
AccessioniPrimary (citable) accession number: O26149
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health