O26147 (RPON_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit N
EC=2.7.7.6

Alternative name(s):
RPB10

Gene names

Name:	rpoN
Ordered Locus Names:	MTH_40

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	55 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00250
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00250
Sequence similarities	Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 55

55

DNA-directed RNA polymerase subunit N HAMAP MF_00250

PRO_0000121353

Sites

Metal binding

6

1

Zinc

Metal binding

9

1

Zinc

Metal binding

43

1

Zinc

Metal binding

44

1

Zinc

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

55

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Helix

14 – 26

13

Helix

30 – 37

8

Helix

42 – 48

7

Turn

49 – 51

3

Sequences

	Sequence		Length	Mass (Da)
	O26147 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A858F89CB5409C95	FASTA	55	6,434
	10 20 30 40 50 MIPVRCLSCG KPVSAYFNEY QRRVADGEDP KDVLDDLGLK RYCCRRMLIS HVETW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.
[2]	"Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacteriumthermoautotrophicum." Mackereth C.D., Arrowsmith C.H., Edwards A.M., McIntosh L.P. Proc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000) [PubMed: 10841539] [Abstract] Cited for: STRUCTURE BY NMR. Strain: Delta H.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000666 Genomic DNA. Translation: AAB84548.1.

PIR

A69152.

RefSeq

NP_275184.1. NC_000916.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EF4	NMR	-	A	1-55	[»]

ProteinModelPortal

O26147.

SMR

O26147. Positions 1-55.

ModBase

Search...

Protein-protein interaction databases

STRING

O26147.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1470002.

GenomeReviews

Gene locus MTH_40 in contig AE000666_GR.

KEGG

mth:MTH40.

NMPDR

fig|187420.1.peg.39.

Phylogenomic databases

eggNOG

arNOG12678.

HOGENOM

HBG391971.

OMA

KYCCRRM.

PhylomeDB

O26147.

ProtClustDB

PRK04016.

Enzyme and pathway databases

BioCyc

MTHE187420:MTH40-MONOMER.

Family and domain databases

HAMAP

MF_00250. RNApol_arch_N.
[Tree]

InterPro

IPR012287. Homeodomain-rel.
IPR000268. RNA_pol_su_N/RPB10.
IPR023580. RNA_pol_su_RPB10.
IPR020789. RNA_pol_suN_Zn-BS.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

KO

K03058.

PANTHER

PTHR23413:SF2. RNA_pol_N/8_sub. 1 hit.

Pfam

PF01194. RNA_pol_N. 1 hit.
[Graphical view]

PIRSF

PIRSF005653. RNA_pol_N/8_sub. 1 hit.

ProDom

PD006539. RNA_pol_N/8_sub. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF46924. RNA_pol_N/8_sub. 1 hit.

PROSITE

PS01112. RNA_POL_N_8KD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPON_METTH

Accession

Primary (citable) accession number: O26147

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program