O26144 (RPOD_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit D
EC=2.7.7.6

Gene names

Name:	rpoD
Ordered Locus Names:	MTH_37

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00320
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00320
Sequence similarities	Belongs to the archaeal rpoD/eukaryotic RPB3 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	DNA-directed RNA polymerase subunit D HAMAP MF_00320		PRO_0000132757

Sequences

	Sequence		Length	Mass (Da)
	O26144 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7740A65424B6AFEB	FASTA	264	29,395
	10 20 30 40 50 60 MDIALKEKTD TEMVFVVSGV TVPFINAIRR ICMMEVPKLA IEYVNMYRND AKMFDEVLAH 70 80 90 100 110 120 RLGLVPLAAD PEFAESLKMP EECDCEEYCS ECSVSLTLRK KGPGVVYSGD LVSETPAVKP 130 140 150 160 170 180 VYPDIPLVKL GDDDELELEA VAQLGVGREH AKWEPTTACA YKYYPRIEFS EDCDECLECI 190 200 210 220 230 240 EACPRDVLGE ESGKPVVVDL ENCSMCKSCV RACDKRAIDV GYEEGKFIFR IETDGSVDPK 250 260 DVLLKACDIL RDKAEQVITF CEGG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB84545.1.
PIR	G69147.
RefSeq	NP_275181.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26144.
ModBase	Search...
Protein-protein interaction databases
STRING	O26144.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1469999.
GenomeReviews	Gene locus MTH_37 in contig AE000666_GR.
KEGG	mth:MTH37.
NMPDR	fig|187420.1.peg.36.
Phylogenomic databases
eggNOG	arNOG06868.
HOGENOM	HBG539019.
OMA	KWQAGVA.
PhylomeDB	O26144.
ProtClustDB	PRK00783.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH37-MONOMER.
Family and domain databases
HAMAP	MF_00320. RNApol_arch_D. [Tree]
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS. IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR022842. DNA-dir_RNA_pol_RpoD_arc. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
KO	K03047.
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
SUPFAM	SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. Uncertain. PS51379. 4FE4S_FER_2. 2 hits. Uncertain. PS00446. RNA_POL_D_30KD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOD_METTH

Accession

Primary (citable) accession number: O26144

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program