O26140 (TRUB_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA pseudouridine synthase B
EC=5.4.99.25

Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase

Gene names

Name:	truB
Ordered Locus Names:	MTH_32

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP MF_01081
Catalytic activity	tRNA uridine(55) = tRNA pseudouridine(55). HAMAP MF_01081
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 2 subfamily. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Probable tRNA pseudouridine synthase B HAMAP MF_01081		PRO_0000121963
Regions
	Domain	239 – 314	76	PUA
Sites
	Active site	72	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O26140 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C94AEE8DF3F1987E	FASTA	324	35,732
	10 20 30 40 50 60 MQVSLMANFI ELKEATTNPD YGCPPAERDI EAHISMGVVN LDKPSGPTSH QVDSWVRDML 70 80 90 100 110 120 HVEKVGHGGT LDPKVTGVLP LGIDRATRVM QLLLEAPKEY VCLMRVHREV DEERIREVLG 130 140 150 160 170 180 EFQGKIFQIP PLKSAVKRDL RVRTIYRVDI LEVDGQDVLF RIACEAGTYV RKYCHDVGEA 190 200 210 220 230 240 LGAGAHMAEL RRTAVGPFTE EGLVTLHDLK DAYQFWVEDG DETFLRECIL PMEFAVGHLP 250 260 270 280 290 300 RVVILDSAVD AICHGADLAR GGIAGLDDNI AWGDTVAIMT LKGELVGVGE ASMSALDIAA 310 320 ADGGLVIETR KVFMEPGTYP RMWR

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB84541.1.
PIR	A69141.
RefSeq	NP_275177.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O26140.
SMR	O26140. Positions 12-323.
ModBase	Search...
Protein-protein interaction databases
STRING	O26140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1469994.
GenomeReviews	Gene locus MTH_32 in contig AE000666_GR.
KEGG	mth:MTH32.
NMPDR	fig|187420.1.peg.32.
Phylogenomic databases
eggNOG	arNOG04654.
HOGENOM	HBG397258.
OMA	IYQRPPL.
PhylomeDB	O26140.
ProtClustDB	PRK04270.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH32-MONOMER.
Family and domain databases
HAMAP	MF_01081. TruB_arch. [Tree]
InterPro	IPR012960. Dyskerin-like. IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR004802. Pseudouridine_synthase-related. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
KO	K03177.
PANTHER	PTHR23127. PTHR23127. 1 hit.
Pfam	PF08068. DKCLD. 1 hit. PF01472. PUA. 1 hit. PF01509. TruB_N. 1 hit. [Graphical view]
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR00425. CBF5. 1 hit.
PROSITE	PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRUB_METTH

Accession

Primary (citable) accession number: O26140

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program