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Protein

Transcription-repair-coupling factor

Gene

mfd

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi512 – 519ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1541-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription-repair-coupling factorUniRule annotation (EC:3.6.4.-UniRule annotation)
Short name:
TRCFUniRule annotation
Gene namesi
Name:mfdUniRule annotation
Ordered Locus Names:HP_1541
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001021681 – 999Transcription-repair-coupling factorAdd BLAST999

Proteomic databases

PaxDbiO26066

Interactioni

Protein-protein interaction databases

DIPiDIP-3189N
IntActiO26066, 8 interactors
MINTiO26066
STRINGi85962.HP1541

Structurei

3D structure databases

ProteinModelPortaliO26066
SMRiO26066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini499 – 656Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini677 – 833Helicase C-terminalUniRule annotationAdd BLAST157

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi609 – 612DEEH box4

Sequence similaritiesi

In the N-terminal section; belongs to the UvrB family.UniRule annotation
In the C-terminal section; belongs to the helicase family. RecG subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JA2 Bacteria
COG1197 LUCA
KOiK03723
OMAiHLPNANT

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.90.1150.50, 1 hit
HAMAPiMF_00969 TRCF, 1 hit
InterProiView protein in InterPro
IPR036101 CarD-like/TRCF_dom_sf
IPR003711 CarD-like/TRCF_domain
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR004576 Mfd
IPR027417 P-loop_NTPase
IPR037235 TRCF-like_C
IPR005118 TRCF_C
PfamiView protein in Pfam
PF02559 CarD_CdnL_TRCF, 1 hit
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF03461 TRCF, 1 hit
SMARTiView protein in SMART
SM01058 CarD_TRCF, 1 hit
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00982 TRCF, 1 hit
SUPFAMiSSF141259 SSF141259, 1 hit
SSF143517 SSF143517, 1 hit
SSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00580 mfd, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

O26066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQSSLYRAL NKGFDYQILA CKDFKESELA KEVISYFKPN TKAILFPEFR
60 70 80 90 100
AKKNDDLRSF FEEFLQLLGG LREFYQALEN KQETIIIAPI SALLHPLPKK
110 120 130 140 150
ELLESFKITL LEKYNLKDLK DKLFYYGYEI LDLVEVEGEA SFRGDIVDIY
160 170 180 190 200
APNSKAYRLS FFDTECESIK EFDPITQMSL KEDLLEIEIP PTLFSLDESS
210 220 230 240 250
YKDLKTKVEQ SPLNSFSKDL TSFGLWFLGE KAQDLLIVYK SIISPRALEE
260 270 280 290 300
IQELASLNEL DCERFKFLKV LENAQGYEDL EIHAHALEGF IALHSNHKIT
310 320 330 340 350
LLAPNKTILD NAISALDAGN MECVIAPFVL NFKTPDGIFI SLNSFERKKK
360 370 380 390 400
RQKSKLALNE LNPGEWVVHD DYGVGVFSQL VQHSVLGSKR DFLEIAYLGE
410 420 430 440 450
DKLLLPVENL HLIARYVAQS DSVPAKDRLG KGSFLKLKAK VRTKLLEIAS
460 470 480 490 500
KIIELAAERN LILGKKMDVH LAELEVFKSH AGFEYTSDQE KAIAEISKDL
510 520 530 540 550
SSHRVMDRLL SGDVGFGKTE VAMHAIFCAF LNGFQSALVV PTTLLAHQHF
560 570 580 590 600
ETLRARFENF GVKVARLDRY ASEKNKLLKA VELGQVDALI GTHAILGAKF
610 620 630 640 650
KNLGLVVVDE EHKFGVKQKE ALKELSKSVH FLSMSATPIP RTLNMALSQI
660 670 680 690 700
KGISSLKTPP TDRKPSRTFL KEKNDELLKE IIYRELRRNG QIFYIHNHIA
710 720 730 740 750
SILKVKTKLE DLIPKLKIAI LHSQINANES EEIMLEFAKG NYQVLLCTSI
760 770 780 790 800
VESGIHLPNA NTIIIDNAQN FGLADLHQLR GRVGRGKKEG FCYFLIEDQK
810 820 830 840 850
SLNEQALKRL LALEKNSYLG SGESVAYHDL EIRGGGNLLG QDQSGHIKNI
860 870 880 890 900
GYALYTRMLE DAIYELSGGK KRLEKSVEIQ LGVSAFLNPE LIASDSLRLD
910 920 930 940 950
LYRRLSLCEN TDEVGQIHEE IEDRFGKIDD LSAQFLQIIT LKILANQLGI
960 970 980 990
IKLSNFNQNI TITYSDEKKE SLKAPSKDDN DILETLLKHL RAQISLKRR
Length:999
Mass (Da):113,090
Last modified:January 1, 1998 - v1
Checksum:iEE550EFB2A2946E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD08581.1
PIRiE64712
RefSeqiNP_208332.1, NC_000915.1
WP_000616334.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD08581; AAD08581; HP_1541
GeneIDi899750
KEGGiheo:C694_07985
hpy:HP1541
PATRICifig|85962.47.peg.1657

Similar proteinsi

Entry informationi

Entry nameiMFD_HELPY
AccessioniPrimary (citable) accession number: O26066
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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