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Protein

Thymidylate synthase ThyX

Gene

thyX

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate.

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.

Cofactori

FADNote: Binds 1 FAD per subunit.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15768.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase ThyX (EC:2.1.1.148)
Short name:
TS
Short name:
TSase
Gene namesi
Name:thyX
Ordered Locus Names:HP_1533
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841S → A: Abolishes complementation when expressed in E.coli. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Thymidylate synthase ThyXPRO_0000175566Add
BLAST

Proteomic databases

PaxDbiO26061.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiO26061. 12 interactions.
MINTiMINT-160429.
STRINGi85962.HP1533.

Structurei

Secondary structure

1
208
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi12 – 2211Combined sources
Helixi25 – 273Combined sources
Beta strandi30 – 323Combined sources
Helixi33 – 4311Combined sources
Helixi46 – 549Combined sources
Beta strandi56 – 6510Combined sources
Helixi66 – 727Combined sources
Beta strandi77 – 826Combined sources
Helixi85 – 884Combined sources
Helixi89 – 924Combined sources
Helixi103 – 1097Combined sources
Turni110 – 1123Combined sources
Helixi119 – 13719Combined sources
Helixi143 – 1464Combined sources
Helixi147 – 1493Combined sources
Beta strandi154 – 16310Combined sources
Helixi164 – 17411Combined sources
Helixi181 – 19313Combined sources
Turni196 – 1983Combined sources
Helixi199 – 2013Combined sources
Helixi203 – 2053Combined sources
Turni206 – 2083Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AH5X-ray2.50A/B/C/D/E/F1-208[»]
ProteinModelPortaliO26061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO26061.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 208208ThyXAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 8411ThyX motifAdd
BLAST

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.Curated

Phylogenomic databases

eggNOGiENOG41087GE. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiCWQSFDK.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O26061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVICKHYTP LDIASQAIRT CWQSFEYSDD GGCKDKELIH RVGNIFRHSS
60 70 80 90 100
TLEHLYYNFE IKGLSRGALQ ELSRHRIASL SVKSSRYTLR ELKEVESFLP
110 120 130 140 150
LNETNLERAK EFLVFVDNEK VNAMSVLALE NLRILLSEHN IKNDLAKYAM
160 170 180 190 200
PESYKTHLAY SINARSLQNF LTLRSSNKAL KEMQDLAKAL FDALPGEHQY

LFEDCLKH
Length:208
Mass (Da):24,071
Last modified:July 11, 2001 - v2
Checksum:i88FF23ED4B26283C
GO

Sequence cautioni

The sequence AAD08571 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08571.1. Different initiation.
PIRiE64711.
RefSeqiNP_208323.1. NC_000915.1.
WP_000451918.1. NC_018939.1.
WP_010875637.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD08571; AAD08571; HP_1533.
GeneIDi899757.
KEGGiheo:C694_07940.
hpy:HP1533.
PATRICi20594487. VBIHelPyl33062_1611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08571.1. Different initiation.
PIRiE64711.
RefSeqiNP_208323.1. NC_000915.1.
WP_000451918.1. NC_018939.1.
WP_010875637.1. NC_000915.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AH5X-ray2.50A/B/C/D/E/F1-208[»]
ProteinModelPortaliO26061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO26061. 12 interactions.
MINTiMINT-160429.
STRINGi85962.HP1533.

Proteomic databases

PaxDbiO26061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08571; AAD08571; HP_1533.
GeneIDi899757.
KEGGiheo:C694_07940.
hpy:HP1533.
PATRICi20594487. VBIHelPyl33062_1611.

Phylogenomic databases

eggNOGiENOG41087GE. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiCWQSFDK.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15768.

Miscellaneous databases

EvolutionaryTraceiO26061.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHYX_HELPY
AccessioniPrimary (citable) accession number: O26061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 11, 2001
Last modified: September 7, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

ThyX activity is mechanistically distinct from ThyA activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.