Skip Header

Contribute Send feedback
Read comments (?) or add your own

O26061 (THYX_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase ThyX
Short name=TS
Short name=TSase
EC=2.1.1.148
Gene names
Name:thyX
Ordered Locus Names:HP_1533
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate. HAMAP-Rule MF_01408

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+. HAMAP-Rule MF_01408

Cofactor

Binds 1 FAD per subunit.

Subunit structure

Homotetramer.

Miscellaneous

ThyX activity is mechanistically distinct from ThyA activity. HAMAP-Rule MF_01408

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Contains 1 thyX (flavin-dependent thymidylate synthase) domain.

Sequence caution

The sequence AAD08571.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 208208Thymidylate synthase ThyX HAMAP-Rule MF_01408
PRO_0000175566

Regions

Domain1 – 208208ThyX
Motif74 – 8411ThyX motif HAMAP-Rule MF_01408

Experimental info

Mutagenesis841S → A: Abolishes complementation when expressed in E.coli. Ref.2

Secondary structure

1 ...................................... 208
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O26061 [UniParc].

Last modified July 11, 2001. Version 2.
Checksum: 88FF23ED4B26283C

FASTA20824,071
        10         20         30         40         50         60 
MEVICKHYTP LDIASQAIRT CWQSFEYSDD GGCKDKELIH RVGNIFRHSS TLEHLYYNFE 

        70         80         90        100        110        120 
IKGLSRGALQ ELSRHRIASL SVKSSRYTLR ELKEVESFLP LNETNLERAK EFLVFVDNEK 

       130        140        150        160        170        180 
VNAMSVLALE NLRILLSEHN IKNDLAKYAM PESYKTHLAY SINARSLQNF LTLRSSNKAL 

       190        200 
KEMQDLAKAL FDALPGEHQY LFEDCLKH

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000511 Genomic DNA. Translation: AAD08571.1. Different initiation.
PIRE64711.
RefSeqNP_208323.1. NC_000915.1.
YP_006935458.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AH5X-ray2.50A/B/C/D/E/F1-208[»]
ProteinModelPortalO26061.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-160429.
STRING85962.HP1533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08571; AAD08571; HP_1533.
GeneID13870751.
899757.
KEGGheo:C694_07940.
hpy:HP1533.
PATRIC20594487. VBIHelPyl33062_1611.

Phylogenomic databases

eggNOGCOG1351.
KOK03465.
OMAYKTELTW.
ProtClustDBPRK00847.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15768.

Family and domain databases

HAMAPMF_01408. ThyX.
InterProIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMSSF69796. Thy1. 1 hit.
TIGRFAMsTIGR02170. thyX. 1 hit.
PROSITEPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO26061.

Entry information

Entry nameTHYX_HELPY
AccessionPrimary (citable) accession number: O26061
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: July 11, 2001
Last modified: May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents