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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei144UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei150UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei152UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei340Meso-diaminopimelateUniRule annotation1
Binding sitei415Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei419Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 80ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1494-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:HP_1494
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019021 – 447UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseAdd BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Proteomic databases

PaxDbiO26027
PRIDEiO26027

Interactioni

Protein-protein interaction databases

IntActiO26027, 1 interactor
STRINGi85962.HP1494

Structurei

3D structure databases

ProteinModelPortaliO26027
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 118UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni364 – 367Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi364 – 367Meso-diaminopimelate recognition motif4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEN Bacteria
COG0769 LUCA
KOiK01928
OMAiCFMEVSS

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR018109 Folylpolyglutamate_synth_CS
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit

Sequencei

Sequence statusi: Complete.

O26027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKKTLTYQ NHTYSFLSDN THEVLENPKE ILFVKTPLNE KYSHLIAEKN
60 70 80 90 100
LAILDFNELK NYFDFKIKIV GITGTNGKTT TASLMYSLLL DLNKKTALLG
110 120 130 140 150
TRGFFINNER IKEKGLTTPT LLELYSDLEE AVRLKCEYFI MEVSSHAIVQ
160 170 180 190 200
KRIAGLDFAL KILTNITSDH LDFHQSIENY RDAKNSFFKD EGLKVINRDE
210 220 230 240 250
TNALFNPVNA HTYALDKKAH LNVQAFSLNP SISASLCYQQ DLRDPNFKEI
260 270 280 290 300
ALMHSPLLGR YNLYNILAGV LGVKLLTQLP LETIVPLLEN FYGVKGRLEI
310 320 330 340 350
VHSKPLVVVD FAHTIDGMQQ VFESFKNQKI TALFGAGGDR DKTKRPEMGA
360 370 380 390 400
IASYYAHKII LTSDNPRSEN EEDIIKDILK GINDSSKVIV EKDRKKAILN
410 420 430 440
ALENLKDDEV LLILGKGDEN IQIFKDKTIF FSDQEVVKSY YQHLKQG
Length:447
Mass (Da):50,858
Last modified:January 1, 1998 - v1
Checksum:i296349E8FE90AD34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD08535.1
PIRiF64706
RefSeqiNP_208285.1, NC_000915.1
WP_000768790.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD08535; AAD08535; HP_1494
GeneIDi899798
KEGGiheo:C694_07740
hpy:HP1494
PATRICifig|85962.47.peg.1606

Similar proteinsi

Entry informationi

Entry nameiMURE_HELPY
AccessioniPrimary (citable) accession number: O26027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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