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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei38 – 381S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei58 – 581S-adenosyl-L-methionineUniRule annotation
Binding sitei86 – 861S-adenosyl-L-methionineUniRule annotation
Binding sitei101 – 1011S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
  2. RNA binding Source: UniProtKB-KW
  3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPY:HP1431-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:HP_1431
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Ribosomal RNA small subunit methyltransferase APRO_0000101540Add
BLAST

Proteomic databases

PRIDEiO25972.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP1431.

Structurei

3D structure databases

ProteinModelPortaliO25972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0030.
KOiK02528.
OMAiYYIATRL.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25972-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVVAKKSLGQ HFLTDESFLD RIVNALPPLN PLKLVEIGVG LGDLTLKLLD
60 70 80 90 100
RYPLKTYEID SHLCEKMRSK LKAQKKPFKL ELVEKDALFL KEEEPYFLIS
110 120 130 140 150
NLPYYIATRL VLNAFKDPKC RGLLVMTQKE VALKFCAKDS QNALSVLAHT
160 170 180 190 200
IGNATLLFDV PPSAFSPPPK VFSSVFEVIK EPLKEKALAS LAQAPFFEEA
210 220 230 240 250
LQKGFEMLED FLKACFSSPR KTLSNNLKKS VSYREKLDKV LDFLALENQP
260 270
TSVRASEIKD YLKLLNYLLK G
Length:271
Mass (Da):30,644
Last modified:January 1, 1998 - v1
Checksum:i870D11C671A7DB91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08470.1.
PIRiG64698.
RefSeqiNP_208222.1. NC_000915.1.
YP_006935352.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08470; AAD08470; HP_1431.
GeneIDi13870641.
899864.
KEGGiheo:C694_07400.
hpy:HP1431.
PATRICi20594261. VBIHelPyl33062_1500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08470.1.
PIRiG64698.
RefSeqiNP_208222.1. NC_000915.1.
YP_006935352.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP1431.

Proteomic databases

PRIDEiO25972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08470; AAD08470; HP_1431.
GeneIDi13870641.
899864.
KEGGiheo:C694_07400.
hpy:HP1431.
PATRICi20594261. VBIHelPyl33062_1500.

Phylogenomic databases

eggNOGiCOG0030.
KOiK02528.
OMAiYYIATRL.
OrthoDBiEOG66F08Z.

Enzyme and pathway databases

BioCyciHPY:HP1431-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiRSMA_HELPY
AccessioniPrimary (citable) accession number: O25972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.