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O25972 (RSMA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:HP_1431
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_0000101540

Sites

Binding site111S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site131S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site381S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site581S-adenosyl-L-methionine By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1011S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
O25972 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 870D11C671A7DB91

FASTA27130,644
        10         20         30         40         50         60 
MVVAKKSLGQ HFLTDESFLD RIVNALPPLN PLKLVEIGVG LGDLTLKLLD RYPLKTYEID 

        70         80         90        100        110        120 
SHLCEKMRSK LKAQKKPFKL ELVEKDALFL KEEEPYFLIS NLPYYIATRL VLNAFKDPKC 

       130        140        150        160        170        180 
RGLLVMTQKE VALKFCAKDS QNALSVLAHT IGNATLLFDV PPSAFSPPPK VFSSVFEVIK 

       190        200        210        220        230        240 
EPLKEKALAS LAQAPFFEEA LQKGFEMLED FLKACFSSPR KTLSNNLKKS VSYREKLDKV 

       250        260        270 
LDFLALENQP TSVRASEIKD YLKLLNYLLK G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08470.1.
PIRG64698.
RefSeqNP_208222.1. NC_000915.1.
YP_006935352.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25972.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP1431.

Proteomic databases

PRIDEO25972.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08470; AAD08470; HP_1431.
GeneID899864.
KEGGheo:C694_07400.
hpy:HP1431.
PATRIC20594261. VBIHelPyl33062_1500.

Phylogenomic databases

eggNOGCOG0030.
KOK02528.
OMASSLCEKM.
OrthoDBEOG66F08Z.

Enzyme and pathway databases

BioCycHPY:HP1431-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_HELPY
AccessionPrimary (citable) accession number: O25972
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names