Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O25944 (NADK_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:HP_1394
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284NAD kinase HAMAP-Rule MF_00361
PRO_0000120623

Regions

Nucleotide binding70 – 712NAD By similarity
Nucleotide binding139 – 1402NAD By similarity
Nucleotide binding180 – 1856NAD By similarity

Sites

Active site701Proton acceptor By similarity
Binding site1671NAD By similarity
Binding site1691NAD By similarity
Binding site1771NAD; via carbonyl oxygen By similarity
Binding site2361NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O25944 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 92050696C7E655F8

FASTA28431,632
        10         20         30         40         50         60 
MKDSLQTIGV FVRPTHYQNP LFEKLEQAKE WVLKLLEDEG FESFMIDSLD GAKDARLIEK 

        70         80         90        100        110        120 
ADAFLCLGGD GTILGALRMT HSYNKPCFGV RIGNLGFLSA VELNGLKDFL QDFKQDRIKL 

       130        140        150        160        170        180 
EEHLALEGRI GKTSFYAINE IVIAKKKALG VLDIKAYAGH TPFNTYKGDG LIIATPLGST 

       190        200        210        220        230        240 
AYNLSAHGPI VHALSQSYIL TPLCDFSLTQ RPLVLGAEFC LNFCAHEDAL VVIDGQATYD 

       250        260        270        280 
LKANQPLYIQ KSPTTTKLLQ KNSRDYFKVL KEKLLWGESP SKKR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08434.1.
PIRB64694.
RefSeqNP_208185.1. NC_000915.1.
YP_006935318.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25944.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO25944. 4 interactions.
MINTMINT-4074458.
STRING85962.HP1394.

Proteomic databases

PRIDEO25944.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08434; AAD08434; HP_1394.
GeneID899902.
KEGGheo:C694_07200.
hpy:HP1394.
PATRIC20594175. VBIHelPyl33062_1458.

Phylogenomic databases

eggNOGCOG0061.
KOK00858.
OMAGAEFCLN.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycHPY:HP1394-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_HELPY
AccessionPrimary (citable) accession number: O25944
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names