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O25927

- LPXA_HELPY

UniProt

O25927 - LPXA_HELPY

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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid A biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciHPY:HP1375-MONOMER.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:HP_1375
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferasePRO_0000188052Add
BLAST

Proteomic databases

PRIDEiO25927.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi85962.HP1375.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 163Combined sources
Beta strandi48 – 525Combined sources
Beta strandi63 – 653Combined sources
Beta strandi79 – 824Combined sources
Turni99 – 1024Combined sources
Beta strandi103 – 1075Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi181 – 1844Combined sources
Beta strandi189 – 1935Combined sources
Helixi195 – 2017Combined sources
Helixi204 – 21714Combined sources
Beta strandi220 – 2223Combined sources
Helixi224 – 23411Combined sources
Helixi239 – 25012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J2ZX-ray2.10A1-270[»]
ProteinModelPortaliO25927.
SMRiO25927. Positions 2-260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25927.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 724Substrate binding

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1043.
KOiK00677.
OMAiDHIKLND.
OrthoDBiEOG6F81P1.

Family and domain databases

Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25927-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKIAKTAII SPKAEINKGV EIGEFCVIGD GVKLDEGVKL HNNVTLQGHT
60 70 80 90 100
FVGKNTEIFP FAVLGTQPQD LKYKGEYSEL IIGEDNLIRE FCMINPGTEG
110 120 130 140 150
GIKKTLIGDK NLLMAYVHVA HDCVIGSHCI LANGVTLAGH IEIGDYVNIG
160 170 180 190 200
GLTAIHQFVR IAKGCMIAGK SALGKDVPPY CTVEGNRAFI RGLNRHRMRQ
210 220 230 240 250
LLESKDIDFI YALYKRLFRP IPSLRESAKL ELEEHANNPF VKEICSFILE
260 270
SSRGVAYKSS EYSSEEKQEE
Length:270
Mass (Da):29,855
Last modified:January 1, 1998 - v1
Checksum:i3668DF2ED248BA4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08418.1.
PIRiG64691.
RefSeqiNP_208166.1. NC_000915.1.
YP_006935297.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08418; AAD08418; HP_1375.
GeneIDi13870584.
900308.
KEGGiheo:C694_07095.
hpy:HP1375.
PATRICi20594139. VBIHelPyl33062_1440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08418.1 .
PIRi G64691.
RefSeqi NP_208166.1. NC_000915.1.
YP_006935297.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J2Z X-ray 2.10 A 1-270 [» ]
ProteinModelPortali O25927.
SMRi O25927. Positions 2-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85962.HP1375.

Proteomic databases

PRIDEi O25927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08418 ; AAD08418 ; HP_1375 .
GeneIDi 13870584.
900308.
KEGGi heo:C694_07095.
hpy:HP1375.
PATRICi 20594139. VBIHelPyl33062_1440.

Phylogenomic databases

eggNOGi COG1043.
KOi K00677.
OMAi DHIKLND.
OrthoDBi EOG6F81P1.

Enzyme and pathway databases

UniPathwayi UPA00359 ; UER00477 .
BioCyci HPY:HP1375-MONOMER.

Miscellaneous databases

EvolutionaryTracei O25927.

Family and domain databases

Gene3Di 1.20.1180.10. 1 hit.
HAMAPi MF_00387. LpxA.
InterProi IPR029098. Acetyltransf_C.
IPR001451. Hexapep_transf.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view ]
PIRSFi PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMi SSF51161. SSF51161. 1 hit.
TIGRFAMsi TIGR01852. lipid_A_lpxA. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Crystal structure of UDP-N-acetylglucosamine acyltransferase from Helicobacter pylori."
    Lee B.I., Suh S.W.
    Proteins 53:772-774(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH R-3-HYDROXY-ACYL CARRIER PROTEIN, SUBUNIT.

Entry informationi

Entry nameiLPXA_HELPY
AccessioniPrimary (citable) accession number: O25927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3