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O25927

- LPXA_HELPY

UniProt

O25927 - LPXA_HELPY

Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

    Catalytic activityi

    (R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei157 – 1571SubstrateBy similarity

    GO - Molecular functioni

    1. acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid A biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciHPY:HP1375-MONOMER.
    UniPathwayiUPA00359; UER00477.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
    Short name:
    UDP-N-acetylglucosamine acyltransferaseUniRule annotation
    Gene namesi
    Name:lpxAUniRule annotation
    Ordered Locus Names:HP_1375
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferasePRO_0000188052Add
    BLAST

    Proteomic databases

    PRIDEiO25927.

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi85962.HP1375.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi14 – 163
    Beta strandi48 – 525
    Beta strandi63 – 653
    Beta strandi79 – 824
    Turni99 – 1024
    Beta strandi103 – 1075
    Beta strandi175 – 1773
    Beta strandi181 – 1844
    Beta strandi189 – 1935
    Helixi195 – 2017
    Helixi204 – 21714
    Beta strandi220 – 2223
    Helixi224 – 23411
    Helixi239 – 25012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J2ZX-ray2.10A1-270[»]
    ProteinModelPortaliO25927.
    SMRiO25927. Positions 2-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO25927.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 724Substrate binding

    Sequence similaritiesi

    Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1043.
    KOiK00677.
    OMAiDHIKLND.
    OrthoDBiEOG6F81P1.

    Family and domain databases

    Gene3Di1.20.1180.10. 1 hit.
    HAMAPiMF_00387. LpxA.
    InterProiIPR029098. Acetyltransf_C.
    IPR001451. Hexapep_transf.
    IPR010137. Lipid_A_LpxA.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF13720. Acetyltransf_11. 1 hit.
    PF00132. Hexapep. 3 hits.
    [Graphical view]
    PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O25927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIAKTAII SPKAEINKGV EIGEFCVIGD GVKLDEGVKL HNNVTLQGHT    50
    FVGKNTEIFP FAVLGTQPQD LKYKGEYSEL IIGEDNLIRE FCMINPGTEG 100
    GIKKTLIGDK NLLMAYVHVA HDCVIGSHCI LANGVTLAGH IEIGDYVNIG 150
    GLTAIHQFVR IAKGCMIAGK SALGKDVPPY CTVEGNRAFI RGLNRHRMRQ 200
    LLESKDIDFI YALYKRLFRP IPSLRESAKL ELEEHANNPF VKEICSFILE 250
    SSRGVAYKSS EYSSEEKQEE 270
    Length:270
    Mass (Da):29,855
    Last modified:January 1, 1998 - v1
    Checksum:i3668DF2ED248BA4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08418.1.
    PIRiG64691.
    RefSeqiNP_208166.1. NC_000915.1.
    YP_006935297.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08418; AAD08418; HP_1375.
    GeneIDi13870584.
    900308.
    KEGGiheo:C694_07095.
    hpy:HP1375.
    PATRICi20594139. VBIHelPyl33062_1440.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08418.1 .
    PIRi G64691.
    RefSeqi NP_208166.1. NC_000915.1.
    YP_006935297.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J2Z X-ray 2.10 A 1-270 [» ]
    ProteinModelPortali O25927.
    SMRi O25927. Positions 2-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 85962.HP1375.

    Proteomic databases

    PRIDEi O25927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08418 ; AAD08418 ; HP_1375 .
    GeneIDi 13870584.
    900308.
    KEGGi heo:C694_07095.
    hpy:HP1375.
    PATRICi 20594139. VBIHelPyl33062_1440.

    Phylogenomic databases

    eggNOGi COG1043.
    KOi K00677.
    OMAi DHIKLND.
    OrthoDBi EOG6F81P1.

    Enzyme and pathway databases

    UniPathwayi UPA00359 ; UER00477 .
    BioCyci HPY:HP1375-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O25927.

    Family and domain databases

    Gene3Di 1.20.1180.10. 1 hit.
    HAMAPi MF_00387. LpxA.
    InterProi IPR029098. Acetyltransf_C.
    IPR001451. Hexapep_transf.
    IPR010137. Lipid_A_LpxA.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF13720. Acetyltransf_11. 1 hit.
    PF00132. Hexapep. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
    SUPFAMi SSF51161. SSF51161. 1 hit.
    TIGRFAMsi TIGR01852. lipid_A_lpxA. 1 hit.
    PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "Crystal structure of UDP-N-acetylglucosamine acyltransferase from Helicobacter pylori."
      Lee B.I., Suh S.W.
      Proteins 53:772-774(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH R-3-HYDROXY-ACYL CARRIER PROTEIN, SUBUNIT.

    Entry informationi

    Entry nameiLPXA_HELPY
    AccessioniPrimary (citable) accession number: O25927
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3