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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei157SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciHPY:HP1375-MONOMER.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:HP_1375
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001880521 – 270Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST270

Proteomic databases

PaxDbiO25927.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi85962.HP1375.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Beta strandi48 – 52Combined sources5
Beta strandi63 – 65Combined sources3
Beta strandi79 – 82Combined sources4
Turni99 – 102Combined sources4
Beta strandi103 – 107Combined sources5
Beta strandi175 – 177Combined sources3
Beta strandi181 – 184Combined sources4
Beta strandi189 – 193Combined sources5
Helixi195 – 201Combined sources7
Helixi204 – 217Combined sources14
Beta strandi220 – 222Combined sources3
Helixi224 – 234Combined sources11
Helixi239 – 250Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2ZX-ray2.10A1-270[»]
ProteinModelPortaliO25927.
SMRiO25927.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25927.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 72Substrate binding4

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
KOiK00677.
OMAiMAYCHLG.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIAKTAII SPKAEINKGV EIGEFCVIGD GVKLDEGVKL HNNVTLQGHT
60 70 80 90 100
FVGKNTEIFP FAVLGTQPQD LKYKGEYSEL IIGEDNLIRE FCMINPGTEG
110 120 130 140 150
GIKKTLIGDK NLLMAYVHVA HDCVIGSHCI LANGVTLAGH IEIGDYVNIG
160 170 180 190 200
GLTAIHQFVR IAKGCMIAGK SALGKDVPPY CTVEGNRAFI RGLNRHRMRQ
210 220 230 240 250
LLESKDIDFI YALYKRLFRP IPSLRESAKL ELEEHANNPF VKEICSFILE
260 270
SSRGVAYKSS EYSSEEKQEE
Length:270
Mass (Da):29,855
Last modified:January 1, 1998 - v1
Checksum:i3668DF2ED248BA4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08418.1.
PIRiG64691.
RefSeqiNP_208166.1. NC_000915.1.
WP_000034151.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08418; AAD08418; HP_1375.
GeneIDi900308.
KEGGiheo:C694_07095.
hpy:HP1375.
PATRICi20594139. VBIHelPyl33062_1440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08418.1.
PIRiG64691.
RefSeqiNP_208166.1. NC_000915.1.
WP_000034151.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2ZX-ray2.10A1-270[»]
ProteinModelPortaliO25927.
SMRiO25927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP1375.

Proteomic databases

PaxDbiO25927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08418; AAD08418; HP_1375.
GeneIDi900308.
KEGGiheo:C694_07095.
hpy:HP1375.
PATRICi20594139. VBIHelPyl33062_1440.

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
KOiK00677.
OMAiMAYCHLG.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.
BioCyciHPY:HP1375-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO25927.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 2 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPXA_HELPY
AccessioniPrimary (citable) accession number: O25927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.