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O25916

- DNAB_HELPY

UniProt

O25916 - DNAB_HELPY

Protein

Replicative DNA helicase

Gene

dnaB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi203 – 2108ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro
    4. identical protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHPY:HP1362-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicative DNA helicase (EC:3.6.4.12)
    Gene namesi
    Name:dnaB
    Ordered Locus Names:HP_1362
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Replicative DNA helicasePRO_0000102023Add
    BLAST

    Proteomic databases

    PRIDEiO25916.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-7529552,EBI-7529552

    Protein-protein interaction databases

    DIPiDIP-3388N.
    IntActiO25916. 4 interactions.
    MINTiMINT-181617.
    STRINGi85962.HP1362.

    Structurei

    Secondary structure

    1
    488
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2321
    Helixi25 – 273
    Helixi28 – 325
    Helixi37 – 393
    Helixi43 – 5715
    Helixi64 – 696
    Beta strandi73 – 753
    Helixi79 – 868
    Helixi95 – 12026
    Helixi154 – 17118
    Turni172 – 1743
    Helixi184 – 1907
    Beta strandi198 – 2036
    Helixi209 – 22214
    Beta strandi226 – 2349
    Helixi236 – 24813
    Helixi252 – 2576
    Helixi262 – 27716
    Beta strandi280 – 2834
    Helixi290 – 30314
    Beta strandi307 – 31610
    Helixi321 – 3266
    Helixi331 – 34515
    Beta strandi349 – 3546
    Helixi357 – 3615
    Beta strandi362 – 3643
    Helixi369 – 3713
    Beta strandi372 – 3743
    Beta strandi383 – 3897
    Helixi391 – 40717
    Helixi408 – 4103
    Helixi412 – 43120
    Beta strandi434 – 44512
    Beta strandi451 – 4588
    Helixi459 – 4613
    Turni469 – 4713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GXVX-ray2.20A/B1-121[»]
    C101-122[»]
    D98-123[»]
    4A1FX-ray2.50A/B152-488[»]
    ProteinModelPortaliO25916.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO25916.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 471300SF4 helicasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0305.
    KOiK02314.
    OMAiSAMNLIT.
    OrthoDBiEOG6T4RW5.

    Family and domain databases

    Gene3Di1.10.860.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48024. SSF48024. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00665. DnaB. 1 hit.
    PROSITEiPS51199. SF4_HELICASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O25916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE    50
    IALKLHEEDC PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE 100
    IKNASIKRKL FGLANTIREQ ALESAQKSSD ILGAVEREVY ALLNGSTIEG 150
    FRNIKEVLES AMDLITENQR KGSLEVTGIP TGFVQLDNYT SGFNKGSLVI 200
    IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA LRALSDLTSI 250
    NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL 300
    KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII 350
    ALVQLNRSLE NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED 400
    NKIDKLKKEG KIEEAQELYL KVNEERRIHK QNGSIEEAEI IVAKNRNGAT 450
    GTVYTRFNAP FTRYEDMPID SHLEEGQETK VDYDIVTT 488
    Length:488
    Mass (Da):55,726
    Last modified:January 1, 1998 - v1
    Checksum:i6FF84C2DE4ED606F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08402.1.
    PIRiB64690.
    RefSeqiNP_208154.1. NC_000915.1.
    YP_006935284.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08402; AAD08402; HP_1362.
    GeneIDi13870571.
    900325.
    KEGGiheo:C694_07030.
    hpy:HP1362.
    PATRICi20594113. VBIHelPyl33062_1427.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD08402.1 .
    PIRi B64690.
    RefSeqi NP_208154.1. NC_000915.1.
    YP_006935284.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3GXV X-ray 2.20 A/B 1-121 [» ]
    C 101-122 [» ]
    D 98-123 [» ]
    4A1F X-ray 2.50 A/B 152-488 [» ]
    ProteinModelPortali O25916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3388N.
    IntActi O25916. 4 interactions.
    MINTi MINT-181617.
    STRINGi 85962.HP1362.

    Proteomic databases

    PRIDEi O25916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08402 ; AAD08402 ; HP_1362 .
    GeneIDi 13870571.
    900325.
    KEGGi heo:C694_07030.
    hpy:HP1362.
    PATRICi 20594113. VBIHelPyl33062_1427.

    Phylogenomic databases

    eggNOGi COG0305.
    KOi K02314.
    OMAi SAMNLIT.
    OrthoDBi EOG6T4RW5.

    Enzyme and pathway databases

    BioCyci HPY:HP1362-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O25916.

    Family and domain databases

    Gene3Di 1.10.860.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48024. SSF48024. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00665. DnaB. 1 hit.
    PROSITEi PS51199. SF4_HELICASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiDNAB_HELPY
    AccessioniPrimary (citable) accession number: O25916
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3