Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Replicative DNA helicase

Gene

dnaB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi203 – 210ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1362-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Gene namesi
Name:dnaB
Ordered Locus Names:HP_1362
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020231 – 488Replicative DNA helicaseAdd BLAST488

Proteomic databases

PaxDbiO25916.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7529552,EBI-7529552

Protein-protein interaction databases

DIPiDIP-3388N.
IntActiO25916. 4 interactors.
MINTiMINT-181617.
STRINGi85962.HP1362.

Structurei

Secondary structure

1488
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 23Combined sources21
Helixi25 – 27Combined sources3
Helixi28 – 32Combined sources5
Helixi37 – 39Combined sources3
Helixi43 – 57Combined sources15
Helixi64 – 69Combined sources6
Beta strandi73 – 75Combined sources3
Helixi79 – 86Combined sources8
Helixi95 – 120Combined sources26
Helixi154 – 171Combined sources18
Turni172 – 174Combined sources3
Helixi184 – 190Combined sources7
Beta strandi198 – 203Combined sources6
Helixi209 – 222Combined sources14
Beta strandi226 – 234Combined sources9
Helixi236 – 248Combined sources13
Helixi252 – 257Combined sources6
Helixi262 – 277Combined sources16
Beta strandi280 – 283Combined sources4
Helixi290 – 303Combined sources14
Beta strandi307 – 316Combined sources10
Helixi321 – 326Combined sources6
Helixi331 – 345Combined sources15
Beta strandi349 – 354Combined sources6
Helixi357 – 361Combined sources5
Beta strandi362 – 364Combined sources3
Helixi369 – 371Combined sources3
Beta strandi372 – 374Combined sources3
Beta strandi383 – 389Combined sources7
Helixi391 – 407Combined sources17
Helixi408 – 410Combined sources3
Helixi412 – 431Combined sources20
Beta strandi434 – 445Combined sources12
Beta strandi451 – 458Combined sources8
Helixi459 – 461Combined sources3
Turni469 – 471Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GXVX-ray2.20A/B1-121[»]
C101-122[»]
D98-123[»]
4A1FX-ray2.50A/B152-488[»]
4ZC0X-ray6.70A/B/C/D1-488[»]
ProteinModelPortaliO25916.
SMRiO25916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 471SF4 helicasePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CDU. Bacteria.
COG0305. LUCA.
KOiK02314.
OMAiQNMERIV.

Family and domain databases

CDDicd00984. DnaB_C. 1 hit.
Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE
60 70 80 90 100
IALKLHEEDC PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE
110 120 130 140 150
IKNASIKRKL FGLANTIREQ ALESAQKSSD ILGAVEREVY ALLNGSTIEG
160 170 180 190 200
FRNIKEVLES AMDLITENQR KGSLEVTGIP TGFVQLDNYT SGFNKGSLVI
210 220 230 240 250
IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA LRALSDLTSI
260 270 280 290 300
NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL
310 320 330 340 350
KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII
360 370 380 390 400
ALVQLNRSLE NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED
410 420 430 440 450
NKIDKLKKEG KIEEAQELYL KVNEERRIHK QNGSIEEAEI IVAKNRNGAT
460 470 480
GTVYTRFNAP FTRYEDMPID SHLEEGQETK VDYDIVTT
Length:488
Mass (Da):55,726
Last modified:January 1, 1998 - v1
Checksum:i6FF84C2DE4ED606F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1.
PIRiB64690.
RefSeqiNP_208154.1. NC_000915.1.
WP_000349722.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08402; AAD08402; HP_1362.
GeneIDi900325.
KEGGiheo:C694_07030.
hpy:HP1362.
PATRICi20594113. VBIHelPyl33062_1427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1.
PIRiB64690.
RefSeqiNP_208154.1. NC_000915.1.
WP_000349722.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GXVX-ray2.20A/B1-121[»]
C101-122[»]
D98-123[»]
4A1FX-ray2.50A/B152-488[»]
4ZC0X-ray6.70A/B/C/D1-488[»]
ProteinModelPortaliO25916.
SMRiO25916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3388N.
IntActiO25916. 4 interactors.
MINTiMINT-181617.
STRINGi85962.HP1362.

Proteomic databases

PaxDbiO25916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08402; AAD08402; HP_1362.
GeneIDi900325.
KEGGiheo:C694_07030.
hpy:HP1362.
PATRICi20594113. VBIHelPyl33062_1427.

Phylogenomic databases

eggNOGiENOG4105CDU. Bacteria.
COG0305. LUCA.
KOiK02314.
OMAiQNMERIV.

Enzyme and pathway databases

BioCyciHPY:HP1362-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO25916.

Family and domain databases

CDDicd00984. DnaB_C. 1 hit.
Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAB_HELPY
AccessioniPrimary (citable) accession number: O25916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.