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O25916

- DNAB_HELPY

UniProt

O25916 - DNAB_HELPY

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Protein
Replicative DNA helicase
Gene
dnaB, HP_1362
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi203 – 2108ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. identical protein binding Source: IntAct

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1362-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Gene namesi
Name:dnaB
Ordered Locus Names:HP_1362
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Replicative DNA helicase
PRO_0000102023Add
BLAST

Proteomic databases

PRIDEiO25916.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7529552,EBI-7529552

Protein-protein interaction databases

DIPiDIP-3388N.
IntActiO25916. 4 interactions.
MINTiMINT-181617.
STRINGi85962.HP1362.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2321
Helixi25 – 273
Helixi28 – 325
Helixi37 – 393
Helixi43 – 5715
Helixi64 – 696
Beta strandi73 – 753
Helixi79 – 868
Helixi95 – 12026
Helixi154 – 17118
Turni172 – 1743
Helixi184 – 1907
Beta strandi198 – 2036
Helixi209 – 22214
Beta strandi226 – 2349
Helixi236 – 24813
Helixi252 – 2576
Helixi262 – 27716
Beta strandi280 – 2834
Helixi290 – 30314
Beta strandi307 – 31610
Helixi321 – 3266
Helixi331 – 34515
Beta strandi349 – 3546
Helixi357 – 3615
Beta strandi362 – 3643
Helixi369 – 3713
Beta strandi372 – 3743
Beta strandi383 – 3897
Helixi391 – 40717
Helixi408 – 4103
Helixi412 – 43120
Beta strandi434 – 44512
Beta strandi451 – 4588
Helixi459 – 4613
Turni469 – 4713

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GXVX-ray2.20A/B1-121[»]
C101-122[»]
D98-123[»]
4A1FX-ray2.50A/B152-488[»]
ProteinModelPortaliO25916.

Miscellaneous databases

EvolutionaryTraceiO25916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 471300SF4 helicase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0305.
KOiK02314.
OMAiSAMNLIT.
OrthoDBiEOG6T4RW5.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25916-1 [UniParc]FASTAAdd to Basket

« Hide

MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE    50
IALKLHEEDC PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE 100
IKNASIKRKL FGLANTIREQ ALESAQKSSD ILGAVEREVY ALLNGSTIEG 150
FRNIKEVLES AMDLITENQR KGSLEVTGIP TGFVQLDNYT SGFNKGSLVI 200
IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA LRALSDLTSI 250
NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL 300
KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII 350
ALVQLNRSLE NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED 400
NKIDKLKKEG KIEEAQELYL KVNEERRIHK QNGSIEEAEI IVAKNRNGAT 450
GTVYTRFNAP FTRYEDMPID SHLEEGQETK VDYDIVTT 488
Length:488
Mass (Da):55,726
Last modified:January 1, 1998 - v1
Checksum:i6FF84C2DE4ED606F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1.
PIRiB64690.
RefSeqiNP_208154.1. NC_000915.1.
YP_006935284.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08402; AAD08402; HP_1362.
GeneIDi13870571.
900325.
KEGGiheo:C694_07030.
hpy:HP1362.
PATRICi20594113. VBIHelPyl33062_1427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1 .
PIRi B64690.
RefSeqi NP_208154.1. NC_000915.1.
YP_006935284.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GXV X-ray 2.20 A/B 1-121 [» ]
C 101-122 [» ]
D 98-123 [» ]
4A1F X-ray 2.50 A/B 152-488 [» ]
ProteinModelPortali O25916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3388N.
IntActi O25916. 4 interactions.
MINTi MINT-181617.
STRINGi 85962.HP1362.

Proteomic databases

PRIDEi O25916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08402 ; AAD08402 ; HP_1362 .
GeneIDi 13870571.
900325.
KEGGi heo:C694_07030.
hpy:HP1362.
PATRICi 20594113. VBIHelPyl33062_1427.

Phylogenomic databases

eggNOGi COG0305.
KOi K02314.
OMAi SAMNLIT.
OrthoDBi EOG6T4RW5.

Enzyme and pathway databases

BioCyci HPY:HP1362-MONOMER.

Miscellaneous databases

EvolutionaryTracei O25916.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
PROSITEi PS51199. SF4_HELICASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiDNAB_HELPY
AccessioniPrimary (citable) accession number: O25916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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