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O25916

- DNAB_HELPY

UniProt

O25916 - DNAB_HELPY

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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi203 – 2108ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. identical protein binding Source: IntAct

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1362-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Gene namesi
Name:dnaB
Ordered Locus Names:HP_1362
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Replicative DNA helicasePRO_0000102023Add
BLAST

Proteomic databases

PRIDEiO25916.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7529552,EBI-7529552

Protein-protein interaction databases

DIPiDIP-3388N.
IntActiO25916. 4 interactions.
MINTiMINT-181617.
STRINGi85962.HP1362.

Structurei

Secondary structure

1
488
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2321Combined sources
Helixi25 – 273Combined sources
Helixi28 – 325Combined sources
Helixi37 – 393Combined sources
Helixi43 – 5715Combined sources
Helixi64 – 696Combined sources
Beta strandi73 – 753Combined sources
Helixi79 – 868Combined sources
Helixi95 – 12026Combined sources
Helixi154 – 17118Combined sources
Turni172 – 1743Combined sources
Helixi184 – 1907Combined sources
Beta strandi198 – 2036Combined sources
Helixi209 – 22214Combined sources
Beta strandi226 – 2349Combined sources
Helixi236 – 24813Combined sources
Helixi252 – 2576Combined sources
Helixi262 – 27716Combined sources
Beta strandi280 – 2834Combined sources
Helixi290 – 30314Combined sources
Beta strandi307 – 31610Combined sources
Helixi321 – 3266Combined sources
Helixi331 – 34515Combined sources
Beta strandi349 – 3546Combined sources
Helixi357 – 3615Combined sources
Beta strandi362 – 3643Combined sources
Helixi369 – 3713Combined sources
Beta strandi372 – 3743Combined sources
Beta strandi383 – 3897Combined sources
Helixi391 – 40717Combined sources
Helixi408 – 4103Combined sources
Helixi412 – 43120Combined sources
Beta strandi434 – 44512Combined sources
Beta strandi451 – 4588Combined sources
Helixi459 – 4613Combined sources
Turni469 – 4713Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GXVX-ray2.20A/B1-121[»]
C101-122[»]
D98-123[»]
4A1FX-ray2.50A/B152-488[»]
ProteinModelPortaliO25916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 471300SF4 helicasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0305.
KOiK02314.
OMAiSAMNLIT.
OrthoDBiEOG6T4RW5.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25916-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE
60 70 80 90 100
IALKLHEEDC PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE
110 120 130 140 150
IKNASIKRKL FGLANTIREQ ALESAQKSSD ILGAVEREVY ALLNGSTIEG
160 170 180 190 200
FRNIKEVLES AMDLITENQR KGSLEVTGIP TGFVQLDNYT SGFNKGSLVI
210 220 230 240 250
IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA LRALSDLTSI
260 270 280 290 300
NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL
310 320 330 340 350
KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII
360 370 380 390 400
ALVQLNRSLE NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED
410 420 430 440 450
NKIDKLKKEG KIEEAQELYL KVNEERRIHK QNGSIEEAEI IVAKNRNGAT
460 470 480
GTVYTRFNAP FTRYEDMPID SHLEEGQETK VDYDIVTT
Length:488
Mass (Da):55,726
Last modified:January 1, 1998 - v1
Checksum:i6FF84C2DE4ED606F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1.
PIRiB64690.
RefSeqiNP_208154.1. NC_000915.1.
YP_006935284.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08402; AAD08402; HP_1362.
GeneIDi13870571.
900325.
KEGGiheo:C694_07030.
hpy:HP1362.
PATRICi20594113. VBIHelPyl33062_1427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08402.1 .
PIRi B64690.
RefSeqi NP_208154.1. NC_000915.1.
YP_006935284.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GXV X-ray 2.20 A/B 1-121 [» ]
C 101-122 [» ]
D 98-123 [» ]
4A1F X-ray 2.50 A/B 152-488 [» ]
ProteinModelPortali O25916.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3388N.
IntActi O25916. 4 interactions.
MINTi MINT-181617.
STRINGi 85962.HP1362.

Proteomic databases

PRIDEi O25916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08402 ; AAD08402 ; HP_1362 .
GeneIDi 13870571.
900325.
KEGGi heo:C694_07030.
hpy:HP1362.
PATRICi 20594113. VBIHelPyl33062_1427.

Phylogenomic databases

eggNOGi COG0305.
KOi K02314.
OMAi SAMNLIT.
OrthoDBi EOG6T4RW5.

Enzyme and pathway databases

BioCyci HPY:HP1362-MONOMER.

Miscellaneous databases

EvolutionaryTracei O25916.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
PROSITEi PS51199. SF4_HELICASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiDNAB_HELPY
AccessioniPrimary (citable) accession number: O25916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3