Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911SubstrateBy similarity
Binding sitei148 – 1481Substrate1 Publication
Binding sitei158 – 1581Substrate1 Publication
Binding sitei188 – 1881Substrate1 Publication
Binding sitei209 – 2091Substrate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciHPY:HP1355-MONOMER.
BRENDAi2.4.2.19. 2604.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:HP_1355
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]PRO_0000155943Add
BLAST

Proteomic databases

PaxDbiO25909.
PRIDEiO25909.

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers.1 Publication

Protein-protein interaction databases

DIPiDIP-3329N.
IntActiO25909. 3 interactions.
MINTiMINT-182259.
STRINGi85962.HP1355.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512Combined sources
Helixi21 – 244Combined sources
Beta strandi30 – 3910Combined sources
Helixi46 – 5510Combined sources
Beta strandi59 – 635Combined sources
Beta strandi75 – 828Combined sources
Helixi83 – 11432Combined sources
Beta strandi117 – 1226Combined sources
Helixi132 – 1409Combined sources
Turni141 – 1433Combined sources
Turni151 – 1533Combined sources
Beta strandi155 – 1573Combined sources
Helixi159 – 1624Combined sources
Helixi168 – 1758Combined sources
Helixi176 – 1783Combined sources
Beta strandi185 – 1917Combined sources
Helixi192 – 20110Combined sources
Beta strandi204 – 2096Combined sources
Helixi213 – 22614Combined sources
Beta strandi231 – 2377Combined sources
Turni240 – 2423Combined sources
Helixi243 – 2475Combined sources
Turni248 – 2503Combined sources
Beta strandi252 – 2554Combined sources
Helixi258 – 2614Combined sources
Beta strandi268 – 2714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B7NX-ray2.30A/B/C1-273[»]
2B7PX-ray2.51A/B/C1-273[»]
2B7QX-ray3.31A/B/C1-273[»]
ProteinModelPortaliO25909.
SMRiO25909. Positions 1-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25909.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1263Substrate binding
Regioni235 – 2373Substrate binding
Regioni256 – 2583Substrate binding

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
KOiK00767.
OMAiHQATFID.
OrthoDBiEOG6KT2XH.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

O25909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIRTFLERA LKEDLGHGDL FERVLEKDFK ATAFVRAKQE GVFSGEKYAL
60 70 80 90 100
ELLEMTGIEC VQTIKDKERF KPKDALMEIR GDFSMLLKVE RTLLNLLQHS
110 120 130 140 150
SGIATLTSRF VEALNSHKVR LLDTRKTRPL LRIFEKYSVL NGGASNHRLG
160 170 180 190 200
LDDALMLKDT HLRHVKDLKS FLTHARKNLP FTAKIEIECE SFEEAKNAMN
210 220 230 240 250
AGADIVMCDN LSVLETKEIA AYRDAHYPFV LLEASGNISL ESINAYAKSG
260 270
VDAISVGALI HQATFIDMHM KMA
Length:273
Mass (Da):30,803
Last modified:January 1, 1998 - v1
Checksum:i69CF0F73B2553CD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08397.1.
PIRiC64689.
RefSeqiNP_208147.1. NC_000915.1.
WP_000405980.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08397; AAD08397; HP_1355.
GeneIDi900348.
KEGGiheo:C694_06995.
hpy:HP1355.
PATRICi20594097. VBIHelPyl33062_1419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08397.1.
PIRiC64689.
RefSeqiNP_208147.1. NC_000915.1.
WP_000405980.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B7NX-ray2.30A/B/C1-273[»]
2B7PX-ray2.51A/B/C1-273[»]
2B7QX-ray3.31A/B/C1-273[»]
ProteinModelPortaliO25909.
SMRiO25909. Positions 1-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3329N.
IntActiO25909. 3 interactions.
MINTiMINT-182259.
STRINGi85962.HP1355.

Proteomic databases

PaxDbiO25909.
PRIDEiO25909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08397; AAD08397; HP_1355.
GeneIDi900348.
KEGGiheo:C694_06995.
hpy:HP1355.
PATRICi20594097. VBIHelPyl33062_1419.

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
KOiK00767.
OMAiHQATFID.
OrthoDBiEOG6KT2XH.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.
BioCyciHPY:HP1355-MONOMER.
BRENDAi2.4.2.19. 2604.

Miscellaneous databases

EvolutionaryTraceiO25909.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Crystal structure of quinolinic acid phosphoribosyltransferase from Helicobacter pylori."
    Kim M.-K., Im Y.J., Lee J.H., Eom S.H.
    Proteins 63:252-255(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, SUBUNIT.

Entry informationi

Entry nameiNADC_HELPY
AccessioniPrimary (citable) accession number: O25909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.