ID   NIKR_HELPY              Reviewed;         148 AA.
AC   O25896;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   27-MAR-2024, entry version 147.
DE   RecName: Full=Putative nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN   OrderedLocusNames=HP_1338;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- FUNCTION: Transcriptional regulator. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00476};
CC       Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC   -!- INTERACTION:
CC       O25896; O25896: HP_1338; NbExp=4; IntAct=EBI-528005, EBI-528005;
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC       {ECO:0000255|HAMAP-Rule:MF_00476}.
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DR   EMBL; AE000511; AAD08380.1; -; Genomic_DNA.
DR   PIR; B64687; B64687.
DR   RefSeq; NP_208130.1; NC_000915.1.
DR   RefSeq; WP_000380787.1; NC_018939.1.
DR   PDB; 2CA9; X-ray; 2.05 A; A/B=1-148.
DR   PDB; 2CAD; X-ray; 2.30 A; A/B=1-148.
DR   PDB; 2CAJ; X-ray; 2.35 A; A/B=1-148.
DR   PDB; 2WVB; X-ray; 1.90 A; A/B=1-148.
DR   PDB; 2WVC; X-ray; 2.10 A; A/B=1-148.
DR   PDB; 2WVD; X-ray; 2.65 A; A/B/C/D=1-148.
DR   PDB; 2WVE; X-ray; 2.30 A; A/B=1-148.
DR   PDB; 2WVF; X-ray; 1.60 A; A/B=1-148.
DR   PDB; 3LGH; X-ray; 2.37 A; A/B/C/D=1-148.
DR   PDB; 3PHT; X-ray; 2.04 A; A/B=1-148.
DR   PDB; 3QSI; X-ray; 3.08 A; A/B/C/D/E/F/G/H/I/J=61-148.
DR   PDB; 6MRJ; X-ray; 2.80 A; A/B/C/D=1-148.
DR   PDBsum; 2CA9; -.
DR   PDBsum; 2CAD; -.
DR   PDBsum; 2CAJ; -.
DR   PDBsum; 2WVB; -.
DR   PDBsum; 2WVC; -.
DR   PDBsum; 2WVD; -.
DR   PDBsum; 2WVE; -.
DR   PDBsum; 2WVF; -.
DR   PDBsum; 3LGH; -.
DR   PDBsum; 3PHT; -.
DR   PDBsum; 3QSI; -.
DR   PDBsum; 6MRJ; -.
DR   AlphaFoldDB; O25896; -.
DR   BMRB; O25896; -.
DR   SMR; O25896; -.
DR   DIP; DIP-3711N; -.
DR   IntAct; O25896; 3.
DR   MINT; O25896; -.
DR   STRING; 85962.HP_1338; -.
DR   PaxDb; 85962-C694_06905; -.
DR   EnsemblBacteria; AAD08380; AAD08380; HP_1338.
DR   KEGG; hpy:HP_1338; -.
DR   PATRIC; fig|85962.47.peg.1433; -.
DR   eggNOG; COG0864; Bacteria.
DR   InParanoid; O25896; -.
DR   OrthoDB; 9806294at2; -.
DR   PhylomeDB; O25896; -.
DR   EvolutionaryTrace; O25896; -.
DR   Proteomes; UP000000429; Chromosome.
DR   CollecTF; EXPREG_00000670; -.
DR   GO; GO:0032993; C:protein-DNA complex; IDA:CollecTF.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0001217; F:DNA-binding transcription repressor activity; IMP:CollecTF.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:CollecTF.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IEP:CollecTF.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR   CDD; cd22231; RHH_NikR_HicB-like; 1.
DR   Gene3D; 3.30.70.1150; ACT-like. Chain A, domain 2; 1.
DR   Gene3D; 1.10.1220.10; Met repressor-like; 1.
DR   HAMAP; MF_00476; NikR; 1.
DR   InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR   InterPro; IPR045865; ACT-like_dom_sf.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR002145; CopG.
DR   InterPro; IPR022988; Ni_resp_reg_NikR.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR   PANTHER; PTHR34719; NICKEL-RESPONSIVE REGULATOR; 1.
DR   PANTHER; PTHR34719:SF2; NICKEL-RESPONSIVE REGULATOR; 1.
DR   Pfam; PF08753; NikR_C; 1.
DR   Pfam; PF01402; RHH_1; 1.
DR   SUPFAM; SSF55021; ACT-like; 1.
DR   SUPFAM; SSF47598; Ribbon-helix-helix; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Metal-binding; Nickel; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..148
FT                   /note="Putative nickel-responsive regulator"
FT                   /id="PRO_0000139291"
FT   BINDING         88
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         99
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         101
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         107
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   STRAND          10..18
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   HELIX           19..32
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   HELIX           37..50
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   HELIX           51..56
FT                   /evidence="ECO:0007829|PDB:2CA9"
FT   STRAND          64..73
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   STRAND          74..78
FT                   /evidence="ECO:0007829|PDB:3QSI"
FT   HELIX           79..89
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   STRAND          91..101
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   STRAND          103..115
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   HELIX           117..128
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   STRAND          133..141
FT                   /evidence="ECO:0007829|PDB:2WVF"
FT   HELIX           143..145
FT                   /evidence="ECO:0007829|PDB:2WVF"
SQ   SEQUENCE   148 AA;  17147 MW;  9C8A2F803E7822B8 CRC64;
     MDTPNKDDSI IRFSVSLQQN LLDELDNRII KNGYSSRSEL VRDMIREKLV EDNWAEDNPN
     DESKIAVLVV IYDHHQRELN QRMIDIQHAS GTHVLCTTHI HMDEHNCLET IILQGNSFEI
     QRLQLEIGGL RGVKFAKLTK ASSFEYNE
//