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O25883

- FUMC_HELPY

UniProt

O25883 - FUMC_HELPY

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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible addition of water to fumarate to give L-malate.By similarity

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donor/acceptorBy similarity
Active sitei317 – 3171By similarity
Binding sitei318 – 3181SubstrateUniRule annotation
Sitei330 – 3301Important for catalytic activityBy similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fumarate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciHPY:HP1325-MONOMER.
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:HP_1325
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Fumarate hydratase class IIPRO_0000161280Add
BLAST

Proteomic databases

PRIDEiO25883.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3320N.
IntActiO25883. 4 interactions.
MINTiMINT-167949.
STRINGi85962.HP1325.

Structurei

3D structure databases

ProteinModelPortaliO25883.
SMRiO25883. Positions 4-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 993Substrate bindingUniRule annotation
Regioni128 – 1314B siteUniRule annotation
Regioni138 – 1403Substrate bindingUniRule annotation
Regioni186 – 1872Substrate bindingUniRule annotation
Regioni323 – 3253Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0114.
KOiK01679.
OMAiMESFNIH.
OrthoDBiEOG6V1M4M.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25883-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFRIEHDTM GEIKVNDSQY WGAQTQRSLE NFKIGTEKMP KELIGAFAKL
60 70 80 90 100
KRSLAVVNHK LGKLSLEKSQ AIIKACDCIL KGELCGEFPL AIWQTGSGTQ
110 120 130 140 150
TNMNLNEVIA NKATEILGGN FREKKLIHPN DDVNMSQSSN DTFPTAMHIV
160 170 180 190 200
SVLEITHRLL PSLENLLKTF KEKSQQFKEI VKIGRTHLQD ATPLTLGQEF
210 220 230 240 250
SGYASMLEHS KQQILESLEH LRELAIGGTA VGTGLNAHKE LSEKVAEELS
260 270 280 290 300
QFSGVKFVSA PNKFHALTSH DAIAYAHGAF KALAANLMKI ANDIRWLASG
310 320 330 340 350
PRCGLGELNI PENEPGSSIM PGKVNPTQCE AMTMVAVQVM GNDTAIGIAA
360 370 380 390 400
SQGNFELNVF KPVIIYNFLQ SLRLLSDSME SFNIHCASGI EPNREKIDYY
410 420 430 440 450
LHHSLMLVTA LNPHVGYENA AKIAKNAHKK GISLKESALE LKLLSAEDFD
460
KFVVPEKMIG PKA
Length:463
Mass (Da):50,975
Last modified:January 1, 1998 - v1
Checksum:iD5798ADBC0EA0260
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08368.1.
PIRiE64685.
RefSeqiNP_208117.1. NC_000915.1.
YP_006935246.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08368; AAD08368; HP_1325.
GeneIDi13870533.
898748.
KEGGiheo:C694_06840.
hpy:HP1325.
PATRICi20594031. VBIHelPyl33062_1386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD08368.1 .
PIRi E64685.
RefSeqi NP_208117.1. NC_000915.1.
YP_006935246.1. NC_018939.1.

3D structure databases

ProteinModelPortali O25883.
SMRi O25883. Positions 4-459.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3320N.
IntActi O25883. 4 interactions.
MINTi MINT-167949.
STRINGi 85962.HP1325.

Proteomic databases

PRIDEi O25883.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08368 ; AAD08368 ; HP_1325 .
GeneIDi 13870533.
898748.
KEGGi heo:C694_06840.
hpy:HP1325.
PATRICi 20594031. VBIHelPyl33062_1386.

Phylogenomic databases

eggNOGi COG0114.
KOi K01679.
OMAi MESFNIH.
OrthoDBi EOG6V1M4M.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .
BioCyci HPY:HP1325-MONOMER.

Family and domain databases

Gene3Di 1.10.275.10. 1 hit.
HAMAPi MF_00743. FumaraseC.
InterProi IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view ]
PANTHERi PTHR11444. PTHR11444. 1 hit.
Pfami PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view ]
PRINTSi PR00149. FUMRATELYASE.
SUPFAMi SSF48557. SSF48557. 1 hit.
TIGRFAMsi TIGR00979. fumC_II. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiFUMC_HELPY
AccessioniPrimary (citable) accession number: O25883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).By similarity

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3