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O25868 (TRPG_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anthranilate synthase component 2

Short name=AS
Short name=ASII
EC=4.1.3.27
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene names
Name:trpG
Ordered Locus Names:HP_1281
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia By similarity.

Catalytic activity

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.

Subunit structure

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE) By similarity.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194Anthranilate synthase component 2
PRO_0000056884

Regions

Domain2 – 194193Glutamine amidotransferase type-1
Region57 – 593Glutamine binding By similarity
Region134 – 1352Glutamine binding By similarity

Sites

Active site841For GATase activity By similarity
Active site1701For GATase activity By similarity
Active site1721For GATase activity By similarity
Binding site841Glutamine By similarity
Binding site881Glutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
O25868 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A03FD6E279345659

FASTA19421,438
        10         20         30         40         50         60 
MKIFFIDNFD SFSYNLVYEL ECLGYEVAVY QNDIDPSYLM DLMNEESKTP LLFISPGPGN 

        70         80         90        100        110        120 
PNSSGNLLKI IAMAKKKFPI LGICLGLQAL AQSYGAKIIR SKEIVHGKAT AIALKKHAVF 

       130        140        150        160        170        180 
KGLGESMVVG RYHSLMASGL PKNLEVIAEH DNIPMAIVNE EDKILAYQFH PESIMTLQGR 

       190 
ALLEQSVGFL EGLL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08325.1.
PIRA64680.
RefSeqNP_208073.1. NC_000915.1.
YP_006935202.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO25868. 1 interaction.
STRING85962.HP1281.

Proteomic databases

PRIDEO25868.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08325; AAD08325; HP_1281.
GeneID898814.
KEGGheo:C694_06620.
hpy:HP1281.
PATRIC20593939. VBIHelPyl33062_1340.

Phylogenomic databases

eggNOGCOG0512.
KOK01658.
OMAHGKLGAM.
OrthoDBEOG6M9F11.

Enzyme and pathway databases

BioCycHPY:HP1281-MONOMER.
UniPathwayUPA00035; UER00040.

Family and domain databases

Gene3D3.40.50.880. 1 hit.
InterProIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMSSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_HELPY
AccessionPrimary (citable) accession number: O25868
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names