O25868 (TRPG_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component II
EC=4.1.3.27

Alternative name(s):
Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	HP_1281

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Anthranilate synthase component II		PRO_0000056884
Regions
	Domain	2 – 194	193	Glutamine amidotransferase type-1
Sites
	Active site	84	1	By similarity
	Active site	170	1	By similarity
	Active site	172	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25868 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A03FD6E279345659	FASTA	194	21,438
	10 20 30 40 50 60 MKIFFIDNFD SFSYNLVYEL ECLGYEVAVY QNDIDPSYLM DLMNEESKTP LLFISPGPGN 70 80 90 100 110 120 PNSSGNLLKI IAMAKKKFPI LGICLGLQAL AQSYGAKIIR SKEIVHGKAT AIALKKHAVF 130 140 150 160 170 180 KGLGESMVVG RYHSLMASGL PKNLEVIAEH DNIPMAIVNE EDKILAYQFH PESIMTLQGR 190 ALLEQSVGFL EGLL

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08325.1.
PIR	A64680.
RefSeq	NP_208073.1. NC_000915.1. YP_006935202.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25868.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP1281.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08325; AAD08325; HP_1281.
GeneID	13870489. 898814.
KEGG	heo:C694_06620. hpy:HP1281.
PATRIC	20593939. VBIHelPyl33062_1340.
Phylogenomic databases
eggNOG	COG0512.
KO	K01658.
OMA	DNEAMFK.
ProtClustDB	PRK05670.
Enzyme and pathway databases
UniPathway	UPA00035; UER00040.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG/PapA. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. trpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_HELPY

Accession

Primary (citable) accession number: O25868

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program