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Protein

NADH-quinone oxidoreductase subunit I

Gene

nuoI

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi85Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi88Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi92Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi121Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi124Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi127Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi131Iron-sulfur 1 (4Fe-4S)UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciHPY:HP1268-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit IUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit IUniRule annotation
NDH-1 subunit IUniRule annotation
Gene namesi
Name:nuoIUniRule annotation
Ordered Locus Names:HP_1268
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002457131 – 220NADH-quinone oxidoreductase subunit IAdd BLAST220

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiO25858.

Interactioni

Subunit structurei

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

Protein-protein interaction databases

IntActiO25858. 2 interactors.
STRINGi85962.HP1268.

Structurei

3D structure databases

ProteinModelPortaliO25858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 1024Fe-4S ferredoxin-type 1UniRule annotationAdd BLAST32
Domaini112 – 1414Fe-4S ferredoxin-type 2UniRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.UniRule annotation
Contains 2 4Fe-4S ferredoxin-type domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105P3U. Bacteria.
COG1143. LUCA.
KOiK00338.
OMAiRYRAVHN.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009051. Helical_ferredxn.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKQEYKQLP KRAEVHSATE QFKDTVKTSL GLDLFKGLGL TIKEFFSPSV
60 70 80 90 100
TIHYPMEQLP LSPRYRAVHN LQRLLDSGSE RCIGCGLCEK ICTSNCIRII
110 120 130 140 150
THKGEDNRKK IDSYTINLGR CIYCGLCAEV CPELAIVMGN RFENASTQRS
160 170 180 190 200
QYGSKSEFLT SEQDAKNCSH AEFLGFGAVS PNYNERMQAT PLDYVQEPSK
210 220
EESQEETPTN PESNKGDENV
Length:220
Mass (Da):24,715
Last modified:January 1, 1998 - v1
Checksum:i13E5931E015E27CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08312.1.
PIRiD64678.
RefSeqiNP_208060.1. NC_000915.1.
WP_001118555.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08312; AAD08312; HP_1268.
GeneIDi898843.
KEGGiheo:C694_06555.
hpy:HP1268.
PATRICi20593913. VBIHelPyl33062_1327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08312.1.
PIRiD64678.
RefSeqiNP_208060.1. NC_000915.1.
WP_001118555.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25858.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO25858. 2 interactors.
STRINGi85962.HP1268.

Proteomic databases

PaxDbiO25858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08312; AAD08312; HP_1268.
GeneIDi898843.
KEGGiheo:C694_06555.
hpy:HP1268.
PATRICi20593913. VBIHelPyl33062_1327.

Phylogenomic databases

eggNOGiENOG4105P3U. Bacteria.
COG1143. LUCA.
KOiK00338.
OMAiRYRAVHN.

Enzyme and pathway databases

BioCyciHPY:HP1268-MONOMER.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009051. Helical_ferredxn.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOI_HELPY
AccessioniPrimary (citable) accession number: O25858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.